Nmr suite professional 7

Manufactured by Chenomx

Sourced in Canada

The Chenomx NMR Suite Professional 7.7 is a software package designed for the analysis and interpretation of nuclear magnetic resonance (NMR) spectroscopy data. The software provides a set of tools and algorithms for the identification and quantification of metabolites in complex biological samples.

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Lab products found in correlation

Nmr suite professional, by Chenomx (4 mentions)

4 protocols using nmr suite professional 7

Quantitative Metabolite Profiling by NMR

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Proton NMR spectra were processed using Chenomx NMR Suite Professional 7.7 software (Chenomx Inc., Edmonton, Canada): phasing and baseline correction were performed, and the pH was calibrated using the resonances from imidazole. The spectra were referenced to the DSS methyl peak at 0.00 ppm. The same peak was also used as a chemical shape indicator, that is as an internal standard for quantitation.
Metabolites were quantified in the 1D ¹H-NMR spectra of breast extracts using the Profiler module of Chenomx NMR Suite Professional software with an inbuilt 1D spectral library. Quantitation was based on comparing the area of selected metabolite peaks with the area under the DSS methyl peak, which corresponded to a known concentration of 0.5 mmol/L in all samples. The resulting metabolite concentration table (56 metabolites × 15 samples) was exported to Excel where sample identifiers were added.

Cônsolo N.R., Samuelsson L.M., Barbosa L.C., Monaretto T., Moraes T.B., Buarque V.L., Higuera-Padilla A.R., Colnago L.A., Silva S.L., Reis M.M., Fonseca A.C., Araújo C.S., Leite B.G., Roque F.A, & Araújo L.F. (2020). Characterization of chicken muscle disorders through metabolomics, pathway analysis, and water relaxometry: a pilot study. Poultry Science, 99(11), 6247-6257.

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Quantitative Metabolic Profiling using 1D 1H-NMR

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1D ¹H-NMR spectra were processed using Chenomx NMR Suite Professional 7.7 software (Chenomx Inc., Edmonton, Canada): phasing and baseline correction was performed and the pH calibrated using the resonances of imidazole. Spectra were referenced to the DSS methyl peak at 0.00 ppm for quantification of all metabolites. The same peak was also used as a chemical shape indicator (i.e., as an internal standard for quantitation).
Thirty-one metabolites were quantified in the 1D ¹H-NMR spectra of muscle extracts using the Profiler module on the Chenomx NMR Suite Professional software with an in-built 1D spectral library. Quantitation was by comparing the area of selected metabolite peaks with the area under the DSS methyl peak, which corresponded to a known concentration of 0.5 mM in each sample. The resulting metabolite concentration table was exported to Excel where sample identifiers were added.

Cônsolo N.R., da Silva J., Buarque V.L., Higuera-Padilla A., Barbosa L.C., Zawadzki A., Colnago L.A., Saran Netto A., Gerrard D.E, & Silva S.L. (2020). Selection for Growth and Precocity Alters Muscle Metabolism in Nellore Cattle. Metabolites, 10(2), 58.

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Quantitative Metabolite Profiling of Muscle Extracts

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Spectra were processed using the Chenomx NMR Suite Professional 7.7 software (Chenomx Inc., Edmonton, AB, Canada): phasing and baseline correction were performed, and the pH was calibrated using the resonances from imidazole. Spectra were referenced to DSS methyl peaks at 0.00 ppm. The same peak was also used as a chemical shape indicator, i.e., as an internal standard for quantitation. Thirty-nine metabolites were quantified in the ¹D ¹H NMR spectra of LT muscle extracts using the Profiler module of the Chenomx NMR Suite Professional software with an in-built 1D spectral library. Quantitation was based on comparing the area of selected metabolite peaks with the area under the DSS methyl peak, which corresponded to a known concentration of 0.5 mM in all samples. The resulting metabolite concentration tables (37 metabolites × 24 samples) were exported to Excel, where sample identifiers were added.

Gómez J.F., Cônsolo N.R., Antonelo D.S., Beline M., Gagaoua M., Higuera-Padilla A., Colnago L.A., Gerrard D.E, & Silva S.L. (2022). Impact of Cattle Feeding Strategy on the Beef Metabolome. Metabolites, 12(7), 640.

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Quantitative 1D NMR Metabolite Analysis

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The 1D 1 H NMR spectra were processed using Chenomx NMR Suite Professional 7.7 software (Chenomx Inc., Edmonton, Canada). Phasing and baseline correction were performed, and the pH was calibrated using the resonances from imidazole. The spectra were referenced to the DSS methyl peak at 0.00 ppm, which was used as an internal standard for quantitation.
Thirty-one metabolites were quantified in the 1D 1 H NMR spectra of meat extracts using the Profiler module on the Chenomx NMR Suite Professional software with an in-built one-dimensional spectral library. Quantitation was based on comparing the area of selected metabolite peaks with the area under the DSS methyl peak, which corresponds to a known concentration of 0.5 mM in each sample. The resulting metabolite concentration table (31 metabolites × 15 samples per each tenderness class) was exported to Microsoft Excel, where sample identifiers were added.

Antonelo D.S., Gómez J.F.M., Cônsolo N.R.B., Beline M., Colnago L.A., Schilling W., Zhang X., Suman S.P., Gerrard D.E., Balieiro J.C.d.C., & Silva S.d.L.e. (2020). Metabolites and Metabolic Pathways Correlated With Beef Tenderness. Meat and Muscle Biology, 4(1).

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