Each urine sample (100 μL) was mixed with 1 µL of acetic acid and 1 μL of ascorbic acid [16 (link),17 (link)] and incubated at room temperature for 10 min. After tetramethylammonium hydroxide (TMAH) digestion was performed at 90 °C [18 (link),19 (link)], 1.87 mL of water and 1.5 μL of acetic acid were added and centrifuged for 15 min at 4000× g. The supernatant was injected into HPLC-MS/MS (Quattro LC, Micromass, Waters Corporation, Milford, MA, USA) with a 250 mm × 2 mm IonPac AS-21 anion exchange column (Dionex, Sunnyvale, CA, USA). An isocratic mobile phase of 20 mM aqueous methylamine was used at a flow rate of 300 µL/min. Iodide was monitored by the mass transition of m/z 127→m/z 127 for I-. The cone voltage and the collision energy were 40 V and 22 V, respectively. The limit of quantitation for urinary iodide was 5 ng/mL. and the detection frequency was 100%, with the range of 6.2 ng/mL to 1664 ng/mL. Duplicate and matrix spike samples were included in each batch of 50–75 samples analyzed. Iodide was not detected in procedural blanks. Reference urine samples from the Centers for Disease Control and Prevention of known iodide concentration (from EQUIP, Ensuring the Quality of Iodine Procedures) were also analyzed with each batch of samples. Laboratory personnel were masked to all other study data.
Quattro lc micromass
Manufactured by Waters Corporation
Sourced in United States
The QUATTRO-LC (Micromass) is a high-performance liquid chromatography-mass spectrometry (LC-MS) system designed for sensitive and accurate sample analysis. It features a triple quadrupole mass spectrometer that provides precise quantitative and qualitative data for a wide range of applications.
Automatically generated - may contain errors
Lab products found in correlation
Alliance hplc system model 2695, by Waters Corporation (4 mentions)
Xselect csh phenyl hexyl, by Waters Corporation (2 mentions)
Xselect csh phenyl hexyl column, by Waters Corporation (2 mentions)
Cholic acid, by Merck Group (1 mentions)
Chenodeoxycholic acid cdca, by Merck Group (1 mentions)
Xselect csh c18 column, by Waters Corporation (1 mentions)
Xselect csh c18, by Waters Corporation (1 mentions)
2695 hplc, by Waters Corporation (1 mentions)
Zorbax eclipse plus c18, by Agilent Technologies (1 mentions)
Acquity hplc system, by Waters Corporation (1 mentions)
Masslynx software, by Waters Corporation (1 mentions)
Xtt cell proliferation kit, by Roche (1 mentions)
Doxorubicin hydrochloride dox, by Merck Group (1 mentions)
9 protocols using quattro lc micromass
1
Quantitative Urinary Iodide Analysis
2
Quantification of Endogenous Bile Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chenodeoxycholic Acid (CDCA), Cholic Acid (CA), Tauro-CDCA (T-CDCA), TCA and other endogenous BAs were purchased from Sigma-Aldrich (St. Louis, MO). All the studied BAs were identified and quantified by high-pressure liquid chromatography-electrospray-mass spectrometry/mass spectrometry (HPLC-ES-MS/MS) by optimized methods [40] (link) suitable for use in pure standard solution, plasma and liver samples after appropriate clean-up preanalytical procedures. Liquid chromatography analysis was performed using an Alliance HPLC system model 2695 from Waters combined with a triple quadruple mass spectrometer QUATTRO-LC (Micromass; Waters) using an electrospray interface. BAs were separated by elution gradient mode with a mobile phase composed of a mixture ammonium acetate buffer 15 mM, pH 8.0 (Solvent A) and acetonitrile:methanol = 75:25 v/v (Solvent B). Chromatograms were acquired using the mass spectrometer in multiple reactions monitoring mode. Biliary BAs were measured with the Total Bile Acid Assay (Dyazime, Dresden, Deutschland), according to the manufacturer's instructions.
3
Serum Bile Acids Quantification by HPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum BAs were identified and quantified by high-pressure liquid chromatography-electrospray-mass spectrometry/mass spectrometry (HPLC-ES-MS/MS) by optimized methods [41 (link)] suitable for use in pure standard solution and serum samples after appropriate clean-up preanalytical procedures. Liquid chromatography analysis was performed using an Alliance HPLC system model 2695 from Waters combined with a triple quadruple mass spectrometer QUATTRO-LC (Micromass; Waters) using an electrospray interface. The analytical column was a Waters XSelect CSH Phenyl-hexyl column, 5 µm, 150 × 2.1 mm, protected by a self-guard column Waters XSelect CSH Phenyl-hexyl 5 µm, 10 × 2.1 mm. BAs were separated by elution gradient mode with a mobile phase composed of a mixture ammonium acetate buffer 15 mM, pH 8.0 (Solvent A) and acetonitrile:methanol = 75:25 v/v (Solvent B). Chromatograms were acquired using the mass spectrometer in multiple reactions monitoring mode.
4
Quantitative Cecal Bile Acid Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cecal bile acid levels were identified and quantified by high‐pressure liquid chromatography‐electrospray‐mass spectrometry/mass spectrometry (HPLC‐ES‐MS/MS) by recent published method suitable for use in pure standard solution, intestinal content, and stool samples after appropriate pre‐analytical procedures. Liquid chromatography analysis was performed using an Alliance HPLC system model 2695 from Waters combined with a triple quadruple mass spectrometer QUATTRO‐LC (Micromass; Waters) using an electrospray interface. The analytical column was a Waters XSelect CSH C18 column, 5 µm, 150 × 2.1 mm, protected by a self‐guard column Waters XSelect CSH C18 5 µm, 10 × 2.1 mm. BAs were separated by elution gradient mode with a mobile phase composed of a mixture ammonium acetate buffer 15 mM, pH 8.0 (Solvent A) and methanol (Solvent B). Chromatograms were acquired using the mass spectrometer in multiple reaction monitoring mode. Briefly, aliquots of cecal sample homogenate (0.3 g) were extracted with 0.9 ml of isopropanol. The mixture was stirred for 30 min at 37°C, then centrifuged at 800 g for 5 min. The supernatant was then diluted 1:10 (v/v) with 40% isopropanol in 15 mM ammonium acetate at pH 8.00, filtered, transferred to an autosampler vial, and 5 μl injected into the HPLC‐ESI‐MS system.
5
Quantification of Biliary Bile Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Biliary bile acids were measured with a colorimetric kit (Diazyme, Poway, CA) according to manufacturer’s instructions. Serum and hepatic BAs were identified and quantified by high-pressure liquid chromatography-electrospray-mass spectrometry/mass spectrometry (HPLC-ES-MS/MS) by optimized methods41 (link) suitable for use in pure standard solution, plasma and liver samples after appropriate clean-up preanalytical procedures. Liquid chromatography analysis was performed using an Alliance HPLC system model 2695 from Waters combined with a triple quadruple mass spectrometer QUATTRO-LC (Micromass; Waters) using an electrospray interface. The analytical column was a Waters XSelect CSH Phenyl-hexyl column, 5 µm, 150 × 2.1 mm, protected by a self-guard column Waters XSelect CSH Phenyl- hexyl 5 µm, 10 × 2.1 mm. BAs were separated by elution gradient mode with a mobile phase composed of a mixture ammonium acetate buffer 15 mM, pH 8.0 (Solvent A) and acetonitrile:methanol = 75:25 v/v (Solvent B). Chromatograms were acquired using the mass spectrometer in multiple reaction monitoring mode.
6
Characterization of Sapindus saponaria Extract
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The hydroalcoholic extract (ETHOSS) from the pericarps of Sapindus saponaria L. was prepared according to Shinobu-Mesquita et al. [16 (link)]. Briefly, fruits of S. saponaria (400 g) were homogenized, extracted with 9:1 (v/v) mixture of ethanol: H2O, and concentrated under low pressure in a rotary evaporator at 40 °C. After the removal of the solvent, the crude extract was frozen in liquid nitrogen, lyophilized using Alpha 1-2 Martin Christ lyophilizer (Osterode am Harz, Niedersachsen, Germany), and stored frozen in a closed plastic bottle until use. For use, the extract was dissolved in ultrapure water.
Electrospray ionization mass spectrometry (ESI-MS) analysis was carried out using a model mass spectrometer (MICROMASS QUATTRO LC) (Waters Corporation Mildfort, MA, USA). The samples were diluted in methanol (chromatographic grade) and directly injected (10 μL), using nitrogen as the nebulizing and dissolving gas. The capillary tension and cone puller values were optimized for each sample. Masslynx 3.3 (Micro Mass) (Waters Corporation, Milford, MA, USA) was used for operating the equipment and data processing.
Electrospray ionization mass spectrometry (ESI-MS) analysis was carried out using a model mass spectrometer (MICROMASS QUATTRO LC) (Waters Corporation Mildfort, MA, USA). The samples were diluted in methanol (chromatographic grade) and directly injected (10 μL), using nitrogen as the nebulizing and dissolving gas. The capillary tension and cone puller values were optimized for each sample. Masslynx 3.3 (Micro Mass) (Waters Corporation, Milford, MA, USA) was used for operating the equipment and data processing.
7
Analytical Protocol for Environmental Pollutants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The analysis of all samples was conducted using an LC-MS-MS system consisting of a Waters 2695 HPLC additionally equipped with a Waters 2487 UV/Vis detector. The instrumental parameters for the methods are detailed shown in Table S3 (supplementary information). A Waters Micromass Quattro LC served as the quadrupole MS, and its method parameters can be found in Table S4 (supplementary information). The chromatographic setup included a C18 reversed-phase column (Reprosil-Pur 120 C18-AQ, 50 mm × 2 mm, 3 µm) with a pre-column cartridge (10 mm × 2 mm) from Dr. Maisch, along with a ZORBAX Eclipse Plus C18 (250 mm × 4.6 mm, 5 µm) from Agilent. For a single substance analysis, the UV/Vis detector method (ME2 which is modified from method ME1) was used with the measuring range spanned from 190 to 700 nm, with DCF, IBP, BPA, and EE2 detected at wavelengths of 275, 220, 195, and 200 nm, respectively. Method EE3 was developed using the MS detector to separate the sample substance mixture and analyzing the byproducts of parent pollutants (focussing on DCF, BPA). The separating column was set up in an adjustable column oven, and gradient methods were employed, altering the eluent composition over the runtime. Water and acetonitrile (ACN), with the addition of 0.1 % formic acid, were used as eluents.
8
Quantitative Endocannabinoid Analysis by LC-MS
9
Stilbene Compounds Cytotoxicity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dimethyl sulfoxide (DMSO, CAS 67-68-5), cytochalasin B (CytB, CAS 14930-96-2), doxorubicin hydrochloride (DOX, CAS 25316-40-9) and reagents for cell culture and micronucleus tests were purchased from Sigma-Aldrich (St. Louis, USA). Cell Proliferation Kit (XTT) was purchased from Roche (Mannheim, Germany). All solvents were anhydrous and all reactions were performed under an inert atmosphere, unless aqueous. All round-bottom flasks were kept dried in an oven under 100 °C. Flash chromatography used to purify the compounds was done using a Teledyne Isco CombiFlash and LC-MS were obtained from Waters Micromass Quattro LC. The purity of all stilbenes tested was above 95%.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!