N hydroxysulfosuccinimide sulfo nhs

Manufactured by Thermo Fisher Scientific

Sourced in United States

N-hydroxysulfosuccinimide (Sulfo-NHS) is a water-soluble compound that can be used to modify proteins and other molecules. It is commonly used in bioconjugation reactions to introduce reactive groups onto target molecules.

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Lab products found in correlation

1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Thermo Fisher Scientific (21 mentions) Bovine serum albumin (bsa), by Merck Group (7 mentions) Phosphate buffered saline (pbs), by Corning (4 mentions) Polystyrene nanoparticles, by Bangs Laboratories (3 mentions) N ethyl n dimethylaminopropyl carbodiimide edc, by Thermo Fisher Scientific (3 mentions) Bovine serum albumin (bsa), by Sangon (2 mentions) Calmodulin, by Merck Group (2 mentions) Anti calmodulin igg, by Thermo Fisher Scientific (2 mentions) Triton x 100, by Merck Group (2 mentions) Penicillin, by Sartorius (2 mentions) Streptomycin, by Sartorius (2 mentions) Mycoplasma detection kit, by Sartorius (2 mentions) Monoclonal anti cea antibodies t86 66, by Merck Group (2 mentions) Poly l lactic acid mw 2 000 da, by Polysciences (2 mentions) Silver nitrate, by Merck Group (2 mentions) O succinyl 3 hydroxy methyl nicotine nic, by LGC (2 mentions) Lactel 50 50 poly lactic co glycolic acid plga, by Durect (2 mentions) 2 n morpholino ethanesulfonic acid mes buffer, by Merck Group (2 mentions) Oleic acid, by Merck Group (2 mentions) 1 octadecene, by Merck Group (2 mentions)

Close spelling variants of this product

Sulfo-NHS (59 citations) N-hydroxysulfosuccinimide (20 citations) N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS) (9 citations) Sulfo-N-Hydroxysulfosuccinimide (8 citations) N-hydroxysulfosuccinimide (NHS) (3 citations)

46 protocols using n hydroxysulfosuccinimide sulfo nhs

Cytotoxicity Assay Reagent Protocol

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Superoxide dismutase (bovine erythrocyte) (SOD), catalase (bovine liver) (C-3155, aqueous solution), cytochrome c (horse heart), and MEM (M3024, without phenol red) were purchased from Sigma–Aldrich (St. Louis, MO). MEM (with phenol red, 10370021) and trypsin-EDTA were obtained from Life Technologies (Carlsbad, CA) and fetal bovine serum was from Biowest (Nuaillé, France). Propidium iodide (PI) was purchased from Dojindo (Kumamoto, Japan). DEVD-NucView488 (NV) was obtained from Biotium (Hayward, CA). 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) was purchased from Nacalai Tesque (Kyoto, Japan) and N-hydroxysulfosuccinimide (sulfo-NHS) was from Thermo Fisher Scientific (Rockford, IL).

Tamura M., Kunihiro S., Hamashima Y., Yoshioka Y., Tone S, & Kameda K. (2015). An improved superoxide-generating nanodevice for oxidative stress studies in cultured cells. Biotechnology Reports, 6, 45-50.

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Virus-like Particle Immobilization on Polystyrene Microspheres

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Carboxy polystyrene (PS) microspheres were purchased from Unibead Scientific Co. Ltd. (Tianjin, China). N-hydroxysulfosuccinimide (Sulfo-NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) were purchased from Thermo. Phosphate buffer solution (PBS) (pH 7.4), trihydroxymethyl aminomethane-hydrochloric acid (Tris-HCl) and 2-(N-morpholino) ethanesulfonic acid hydrate (MES) buffer (0.05 mol, pH 6.0) were purchased from Leagene Biotechnology (Beijing, China). Dengue virus (DENV), tick-borne encephalitis virus-like particles (TBEV), Japanese encephalitis virus (JEV), and coxsackie virus B3 (CVB3) were obtained from the Fourth Military Medical University. Bovine serum albumin (BSA) and fetal bovine serum (FBS) were purchased from Sangon Biotechnology (Shanghai, China).

Liu X., Chen X., Dong Y., Zhang C., Qu X., Lei Y., Jiang Z, & Wei X. (2023). Multiple virus sorting based on aptamer-modified microspheres in a TSAW device. Microsystems & Nanoengineering, 9, 64.

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Multifunctional Nanoparticle Biosensing

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N-(4-Aminobutyl)-N-ethylisoluminal (ABEI), sodium sulfide nonahydrate (SDS), potassium persulfate (KPS), N, N’-Methylenebisacrylamide (bis), acrylic acid (AA), allylamine (ALA) and N-isopropylacrylamide (NIPAM) were purchased from Sigma Aldrich. The biotinylated mouse anti-human ErbB2/HER2 (Recombinant Monoclonal Human IgG₁ Clone Hu5), mouse anti-human CD63 (Clone mem-259), and biotinylated mouse anti-human CD63 (Clone NVG-2) was obtained from R&D systems, Sino Biological, and BioLegend, respectively. Horseradish peroxidase (HRP)-conjugated secondary antibody was from Cell Signaling Technology; and the streptavidin-HRP-conjugate was attained from Invitrogen. All other chemicals, including 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and N-hydroxysulfosuccinimide (Sulfo-NHS), were purchased from ThermoFisher Scientific.

Jiang Q., Liu Y., Wang L., Adkins G.B, & Zhong W. (2019). Rapid Enrichment and Detection of Extracellular Vesicles Enabled by CuS-Enclosed Microgels. Analytical chemistry, 91(24), 15951-15958.

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Multiplex Immunoassay Bioconjugation Protocol

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tert-butyl 11-azatricyclo [6.2.1.02,7] undeca-2,4,6,9-tetraene-11-carboxylate (ChemScene), BODIPY-TZ-NHS ester (MW 613.24; WuXi AppTec), biotin-PEG-SVA (MW 3400; Laysan Bio), NH₂-PEG-COOH (MW 3400; Laysan Bio), Streptavidin (ProSpec), biotin (Sigma-Aldrich), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC; Pierce), N-hydroxysulfosuccinimide (Sulfo-NHS; Thermo Fisher Scientific), aliphatic amine latex beads (2% w/v, 0.4 μm; Thermo Fisher Scientific), Phosphate Buffered Saline Tablets (PBS; MP Biomedicals), 0.2M carbonate buffers (Alfa Aesar), ELISA MAX Standard Set Human IL-6 (BioLegend), TMB reagents (BioLegend), Amicon 100K cellulose centrifugal filter Unit (Thermo Fisher Scientific), Zeba 7K MWCO Spin Desalting Columns (Thermo Fisher Scientific), HyClone Iscove’s Modified Dulbecco’s Medium (IMDM; GE Healthcare Bio-Sciences), Fetal Bovine Serum (FBS; GE Healthcare Bio-Sciences), trifluoroacetic acid (Sigma-Aldrich), Off-clot human Serum (ZenBio), and Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F12; GE Healthcare Bio-Sciences), 1% penicillin/streptomycin (Thermo Fisher Scientific), LPS (E. coli K12, 1 μg/mL; Invitrogen).

Liu J., Abdullah M.A., Yang L, & Wang J. (2019). Fast Affinity Induced Reaction Sensor (FAIRS) Based on Fluorogenic Click Reaction for Quick Detection of Protein Biomarkers. Analytical chemistry, 92(1), 647-653.

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Immunohistochemical Analysis of Alzheimer's Disease

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The SPIONs (superparamagnetic iron oxide nanoparticles) were from Chemicell GmbH, Berlin, Germany (Type: SiMAG-TCL, Lot # 0808/07, 50 nm nominal hydrodynamic diameter). LD55 was synthesized as reported previously [43 (link)]. Thioflavin-S and resveratrol were obtained from Sigma-Aldrich Corp. (St. Louis, MO, USA). Mouse anti-Tau, clone tau-5, N-hydroxysulfosuccinimide (Sulfo-NHS) and 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) were obtained from Thermo Fisher Scientific (Fremont, CA, USA). Rabbit (polyclonal) anti-Aβ and Rabbit (polyclonal) anti-AβPP antibodies were obtained from Invitrogen Corp. (Camarillo, CA, USA). Rabbit anti-Iba-1 was obtained from Wako (Richmond, VA). The secondary antibody, rhodamine-conjugated donkey anti-rabbit IgG, for fluorescence microscopy, and normal donkey serum used in immunohistochemistry were purchased from JacksonImmuno Research (West Grove, PA, USA). Vectashield DAPI stain was purchased from Vector Laboratories (Burlingame, CA, USA). Paraffin embedding and sectioning were performed by TriCore Reference Laboratories (Albuquerque, NM, USA). Immunohistochemistry was performed by TriCore Reference Laboratories (Albuquerque, NM, USA) using the Ventana BenchMark XT IHC/ISH Staining Module.

Solberg N.O., Chamberlin R., Vigil J.R., Deck L.M., Heidrich J.E., Brown D.C., Brady C.I., Vander Jagt T.A., Garwood M., Bisoffi M., Severns V., Vander Jagt D.L, & Sillerud L.O. (2014). Optical and SPION-Enhanced MR Imaging Shows that trans-Stilbene Inhibitors of NF-κB Concomitantly Lower Alzheimer’s Disease Plaque Formation and Microglial Activation in AβPP/PS-1 Transgenic Mouse Brain. Journal of Alzheimer's disease : JAD, 40(1), 191-212.

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Polystyrene Nanoparticle Functionalization

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Polystyrene nanoparticles were purchased from Bangs Laboratories. Phosphate-buffered saline (PBS) was purchased from Corning and filtered with 0.22 μm filters (Millex). Human plasma IgM and human colostrum IgA were purchased from Athens Research and Technology. Anti-Calmodulin (IgG) was purchased from Invitrogen. Calmodulin and bovine serum albumin (BSA) were purchased from Sigma-Aldrich. N-ethyl-N’-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were purchased from Thermo Fisher Scientific. Dithiolalkanearomatic PEG6-COOH was purchased from Sensopath Technologies. Other chemicals were from Sigma-Aldrich. DI water with resistivity of 18.2 MΩ/cm was filtrated with 0.22 μm filter and used in all experiments.

Zhang P., Ma G., Dong W., Wan Z., Wang S, & Tao N. (2020). Plasmonic Scattering Imaging of Single Proteins and Binding Kinetics. Nature methods, 17(10), 1010-1017.

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Labeling Alginates with Fluorescent Dyes

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Alginates (LF10/60, LF 20/40) were labelled with either Alexa Fluor® 350 hydrazide (AF350) or Alexa Fluor® 555 hydrazide (AF555) (Thermo Fisher, Waltham, MA, USA), using aqueous carbodiimide chemistry. For both, a 1% solution of alginate was prepared in 0.1 M MES buffer (Sigma Aldrich) to which 1-ethyl-(dimethylaminopropyl) carbodiimide (EDC) (Sigma Aldrich), N-hydroxysulfosuccinimide (Sulfo-NHS) (Thermo Fisher) and the fluorescent label were added and stirred in the dark for 18 hours at room temperature. Unconjugated label was removed by ultrafiltration against deionized water. The purified alginate-AF350 and alginate-AF555 conjugates were then filtered (0.22 km, Millipore), lyophilized, and stored at 4 °C until use.

Madrigal J.L., Sharma S.N., Campbell K.T., Stilhano R.S., Gijsbers R, & Silva E.A. (2018). Microgels produced using microfiuidic on-chip polymer blending for controlled released of VEGF encoding lentivectors. Acta biomaterialia, 69, 265-276.

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Lipase-Catalyzed Biodiesel Production

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Lipase from Thermomyces lanuginosus (Lot# L0777, a liquid preparation with 72.75 ± 0.45 U/mg of protein determined by Ref. [16 (link)]), 4-morpholineethanesulfonic acid (MES), n-hexane, methanol, analytical or GC grade FAME (methyl undecanoate, methyl palmitate, methyl linoleate, methyl linolenate, methyl oleate, and methyl stearate) as analysis calibration standards were purchased from Sigma-Aldrich. 1-ethyl-3-(3-dimethylaminopropyl) carbodiimidehydrochloride (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were purchased from Thermo Fisher Scientific. Soybean oil (saponification value of 195.0 ± 0.1 mgKOH/g, acid value of 0.1794 ± 0.0009 mgKOH/g, average molecular weight of 864.1 ± 0.5 g/mol) was purchased from a local market (Taiyuan, China). All other chemicals were guaranteed or analytic grade and were used directly. Water used throughout this work was from a Milli-Q ultrapure water system.

Li J., Zhang J., Shen S., Zhang B, & Yu W.W. (2020). Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis. Materials Today. Communications, 24, 101197.

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Antibody conjugation to magnetic beads

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Nanomagnetic beads (750 nm) (Aorun Micronano New Material Technology Company, Shanghai, China) were washed with morpholine ethanesulfonic acid (Sigma Aldrich, St. Louis, MO, United States) three times at a volume ratio of 1:10 to ensure monodispersion, then 24 μL 1- (3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC-HCl) (200 mM) (Thermo Fisher Scientific Inc., Rockford, United States) and 240 μL N-hydroxysulfosuccinimide (Sulfo-NHS) (200 mM) (Thermo Fisher Scientific Inc.) were added to 1 mg magnetic beads. The magnetic beads were mixed with 40 μg monoclonal antibody for 30 min at 37°C, then 1 mL 1% (w/v) BSA (Sigma Aldrich, St. Louis, MO, United States) blocking buffer was added at 37°C for 2 h and stored at 4°C.

Gao J., Zhang L., Xue L., Cai W., Qin Z., Yang J., Liang Y., Wang L., Chen M., Ye Q., Li Y., Wang J., Wu S., Wu Q, & Zhang J. (2021). Development of a High-Efficiency Immunomagnetic Enrichment Method for Detection of Human Norovirus via PAMAM Dendrimer/SA-Biotin Mediated Cascade-Amplification. Frontiers in Microbiology, 12, 673872.

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Fabrication and Characterization of CEA-Targeted Fluorescent Nanoparticles

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The following analytical-grade chemicals were purchased from commercial sources and were used without further purification: L-glutamic acid (E), L-phenylalanine (F), indiocyaninegreen (ICG), human serum albumin (HSA), bovine plasma fibrinogen, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), Matrigel, Triton-x-100 and monoclonal anti-CEA antibodies (T86-66) from Sigma (Rehovot, Israel); Poly(L-lactic acid) MW 2,000 Da from Polysciences (Warrington, PA, USA); N-hydroxysulfosuccinimide (Sulfo-NHS) and 2-morpholino ethanesulfonic acid (MES, pH 6) from Thermo Fisher Scientific (Rockford, IL, USA); Phosphate Buffered Saline (PBS), Minimum Essential Medium (MEM) eagle, McCoy’s 5A medium and Dulbecco’s modification of eagle’s medium (DMEM), Fetal Bovine Serum (FBS), glutamine, penicillin, streptomycin and mycoplasma detection kit from Biological Industries (Bet Haemek, Israel); cell cytotoxicity assay kit (LDH) from Roche (Switzerland); LS174t, SW480 and HT29 cell lines from American Type Culture Collection (ATCC); donkey anti-rabbit IgG from Jackson ImmunoResearch Laboratories (West Grove, PA, USA); water was purified by passing deionized water through an Elgastat Spectrum reverse osmosis system (Elga Ltd., High Wycombe, UK).

Kolitz-Domb M., Grinberg I., Corem-Salkmon E, & Margel S. (2014). Engineering of near infrared fluorescent proteinoid-poly(L-lactic acid) particles for in vivo colon cancer detection. Journal of Nanobiotechnology, 12, 30.

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