H3k4ac

The antibodies of HDAC1, pSTAT6, p300, pp300, STAT3, pSTAT3, H3K4ac, RNA polymerase II, shRNA kits of p300 and STAT3 were from Santa Cruz Biotech (Santa Cruz, CA). The fluorochrome-labeled antibodies of Foxp3, CD4, IL-10, CD19 and IgE were from BD Biosciences (Franklin Lakes, NJ). The biotinylated IgE antibody was from Abcam (Cambridge, MA). Magnetic cell sorting kits were from Miltenyi Biotech (San Diego, CA). The house dust mite vaccine was from Wowu Biotech (Hangzhou, China). Clostridium butyricum was from Shenzhen Kexing Biotech (Shenzhen, China). The Der p 1 protein was from Dr. Zhijiang Liu (Shenzhen University, China). PCI-32765 was purchased from Chem Blink (Shanghai, China). Reagents for real time RT-PCR and Western blotting were from Invitrogen (Carlsbad, CA). Protein G, ChIP kit and butyrate sodium were from Sigma Aldrich (St. Louis., MO).

The p300 inhibitor garcinol, OVA-specific IgE ELISA kit and total IgE ELISA kit were purchased from the Biomart (Beijing, China). The ELISA kits of IL-4, IL-5, IL-13 were purchased from R&D Systems (Shanghai, China). The antibodies of p300, p300, STAT6, pSTAT6, TIM4, H3K4ac, RNA polymerase II, NCOA1, and the RNAi kits of STAT6 and NCOA1 were purchased from Santa Cruz Biotech (Shanghai, China). The immune cell isolation kits were purchased from Miltenyi Biotech (Shanghai, China). The antibodies of CD45.2 and TIM4 were purchased from BD Biosciences (Shanghai, China). The reagents for RT-qPCR and Western blotting were purchased from Invitrogen (Shanghai, China). The ChIP kit, ovalbumin and cholera toxin were purchased from Sigma Aldrich (Shanghai, China). The endotoxin levels in all reagents were detected using the Limulus assay (Limulus amebocyte lysate QCL 1000, Bio Whittaker, Walkersville, MD, USA). The reagents used in this study contained <0.2U endotoxin/10 μg reagents.