Mcl 1 sc 819 and noxa sc 56169

Whole cell lysates for co-immunoprecipitation or for total protein evaluation were prepared with RIPA buffer supplemented with phosphatase and protease inhibitors and 1% PMSF as previously described.^{16 (link),24 (link)} Antibodies to BCL-x_L (#2764S), BIM (#2933BC), PUMA (#4976) and BCL-2 (#4223S) were purchased from Cell Signaling Technology (Danvers, MA, USA); MCL-1 (sc-819) and NOXA (sc-56169) from Santa Cruz Biotechnology (Dallas, TX, USA) and GAPDH (GTX41027) from GeneTex Inc. (Irvine, CA, USA). Antibodies (used for co-immunoprecipitates) to MCL-1(559027) and BCL-2 were purchased from BD-Pharmingen (BD Biosciences, San Jose, CA, USA). The antibody used for the BCL-x_L IP was generated in the Boise Laboratory (Atlanta, GA, USA).^{50 (link)}