Accu check aviva blood glucose monitor

Animals were starved for 4 h prior to administration of 1 g β-D (+)-glucose/kg bodyweight via oral gavage (n = 25–27) [49 (link)]. Glucose was determined in blood obtained from a small incision in the mouse tail and pressed on a glucometer strip at 0, 15, 30, 60, 90 and 120 min post-gavage. Blood glucose levels were measured using the Accu-Check® Aviva blood glucose monitor (Roche) [48 (link)]. Plasma insulin was measured using an ELISA at 0 and 15 min post-gavage in 4–5 mice per genotype.

ipGTT was performed following a 6 h fast, mice were administered with glucose (1 g/kg) via intraperitoneally (ip) injection. Blood samples were taken from the tail vein at 0, 15, 30, 60 and 120 minutes and measured using an Accu check Aviva blood glucose monitor (Roche Diagnostics, Burgess Hill, UK). For the ipITT, a dose of 0.75 U/kg of regular human insulin was administered intraperitoneally in 4-hour fasted mice. Blood glucose concentrations were measured using the same glucometer as above. Area under the curve (AUC) for the ipGTT and ipITT were measured starting at baseline (0 min) values.