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Bovine serum albumin bsa

Manufactured by Vector Laboratories
Sourced in United States

Bovine serum albumin (BSA) is a widely used protein derived from bovine serum. It serves as a stabilizing agent, blocking reagent, and protein standard in various laboratory applications.

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3 protocols using bovine serum albumin bsa

1

Immunohistochemical Analysis of CD4+ T Cells

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IHC was performed according to the manufacturer's protocol. To retrieve antigens in the skin tissues, the deparaffinized sections were boiled in 10 mM sodium citrate buffer for 30 min. Endogenous peroxidase was subsequently blocked by incubation in 3% H2O2 for 30 min and slides were blocked with 5% normal goat serum (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) in phosphate-buffered saline (PBS) containing 5% fetal bovine serum (Corning, Inc., Corning, NY, USA), 2% bovine serum albumin (BSA; Vector Laboratories, Burlingame, CA, USA) and 0.1% Triton X-100 for 1 h. Following washing in PBS three times, the sections were incubated overnight with an antibody against biotin anti-mouse CD4 (cat. no. 100403; BioLegend, Inc., San Diego, CA, USA), diluted 1:100 in normal goat serum, in a humidified chamber. After washing with PBS, the biotinylated goat anti-mouse IgG (cat. no. BA-9200; diluted 1:200 in TBST; Vector Laboratories) was applied for 2 h at room temperature. The slides were subsequently incubated with an Avidin/Biotinylated Enzyme Complex kit (Vector Laboratories) for 1 h, followed by the substrate chromogen (3,3-diaminobenzidine; Dako, Glostrup, Denmark), and counterstained by hematoxylin staining. CD4 staining was visualized using LAS (magnification, ×200).
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2

Immunofluorescence Staining of Liver Macrophages

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Liver macrophages were isolated for immunofluorescence staining of IL-1 and IL-10 by seeding them into 6-well plates and incubating with different treatments. After incubation, the cells were fixed with cold methanol: acetone (1∶1) for 10 min on ice. After washing the cells with PBS (pH 7.2) and blocking in solution containing 1% immunohistochemical-grade bovine serum albumin (BSA; Vector Laboratories, Burlingame, CA, U.S.A) the cells were incubated overnight with an IL-1 primary antibody (Santa Cruz biotechnology, Dallas, USA) and IL-10 primary antibody (Abcam, Cambridge, USA) at 4°C. After three washes with blocking solution, the sections were incubated for 30 min at room temperature with secondary antibodies (Antgene, Wuhan, China) and viewed by confocal laser microscopy.
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3

Immunostaining and Western Blot Analysis

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BAK, dimethyl sulfoxide (DMSO), and Triton X-100, collagenase I and Lucifer Yellow dye were purchased from Sigma Aldrich (St. Louis, MO); Protein A/G PLUS-Agarose Immunoprecipitation Reagent was from Santa Cruz Biotechnology (Santa Cruz, CA); PVDF Western Blotting Membrane was from Roche (Basel, Switzerland); pentobarbital sodium was from Abbott Laboratories (North Chicago, IL); Enhanced chemiluminescence (ECL) kit was obtained from GE Healthcare UK (Chalfont, UK); Mounting medium with 4, 6-diamidino-2-phenylindole (DAPI) and bovine serum albumin (BSA) were from Vector Laboratories (Burlingame CA); Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Life Technologies (Carlsbad, CA); mouse-anti-rabbit ZO-1 antibody, Alexa488-conjugated donkey-anti-mouse IgG, and Alexa555-conjugated donkey-anti-goat IgG were from Life Technologies (Carlsbad, CA); goat polyclonal antibody for Cx43 and P-Cx43, Horseradish peroxidase (HRP)-conjugated donkey anti- goat IgG were from Santa Cruz Biotechnology (Santa Cruz, CA); mouse anti-rabbit β-actin antibody from Sigma Aldrich (St. Louis, MO); Horseradish peroxidase (HRP)-conjugated goat anti- mouse IgG from Merck (Darmstadt, Germany).
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