Strata x 33 μm polymeric reversed phase 30 mg solid phase extraction cartridge

O-(Pyridin-3-yl-methyl)hydroxylamine (PMOA), butyraldehyde, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), tris(hydroxymethyl)aminomethane (Tris), EDTA, DNase I (type IV, bovine pancreas), alkaline phosphatase (Escherichia coli), nuclease P1 (Penicillium citrinum), RNase A (bovine pancreas), RNase T1 (Aspergillus oryzae), and proteinase K (Tritirachium album) were purchased from Sigma-Aldrich (St. Louis, MO). Phosphodiesterase I (Crotalus adamanteus venom) and adenosine deaminase were purchased from Worthington Biochemical Corp. (Lakewood, NJ). LC-MS grade solvents were purchased from Fisher Chemical Co. (Pittsburgh, PA). Puregene protein precipitation (PP) solution and DNeasy Blood & Tissue kit were purchased from Qiagen (Germantown, MD). N,N-Bis(2-chloroethyl)ethylamine hydrochloride was purchased from Aaron Chemistry Gmbh (Mittenwald, Germany). Pall 10 kDa filter with omega membrane, MWCO 10 kDa, was purchased from Sigma Aldrich (St. Louis, MO) All other chemicals were ACS grade and purchased from Sigma-Aldrich unless otherwise stated. Strata-X 33 μm Polymeric Reversed Phase 30 mg solid-phase extraction (SPE) cartridge was purchased from Phenomenex (Torrance, CA). Sola SCX cartridges were purchased from Thermo Scientific (Bellafonte, PA). MicroLiter autosampler vials with silanized glass inserts were purchased from Wheaton (Millville, NJ).

Reduced glutathione (GSH), oxidized glutathione GSSG, O-(pyridin-3-ylmethyl)hydroxylamine (PMOA), butyraldehyde, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), calf thymus (CT) DNA, p-Benzoquinone (p-BQ), HQ, 2-methylhydroquinone (2-MeHQ), Cat, p-cresol (p-Cre), m-cresol (m-Cre), and o-cresol (o-Cre), Acr, methylacrolein (MeAcr), crotonaldehyde (Crot), Methyl vinyl ketone (MVK), RNase A (bovine pancreas), RNase T1 (Aspergillus oryzae), proteinase K (Tritirachium album), sodium dodecyl sulfate (SDS), DNase I (type IV, bovine pancreas), alkaline phosphatase (Escherichia coli), and nuclease P1 (Penicillium citrinum) were purchased from Sigma Aldrich (St. Louis, MO, USA). Phosphodiesterase I (Crotalus adamanteus venom) and adenosine deaminase were purchased from Worthington Biochemical Corp. (Newark, NJ). LC/MS grade solvents were purchased from Fisher Chemical Co. (Pittsburgh, PA). Puregene protein precipitation solution was purchased from Qiagen (Germantown, MD). Strata-X 33 μm Polymeric Reversed Phase 30 mg solid-phase extraction (SPE) cartridge was obtained from Phenomenex (Torrance, CA, USA). If not stated otherwise, all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).