Flow cytometry was performed using Annexin V-APC/PI Apoptosis Kit (Elabscience, E-CK-A217) according to the manufacturer’s instructions. After digestion, cells were adjusted to a density of 1 × 105 cells/mL with Annexin V Binding Buffer, and incubated with Annexin V-APC Reagent and propidium iodide Reagent staining in the dark at room temperature for 15 min. After incubation, cells were diluted with Annexin V Binding Buffer and analyzed with a flow cytometer (BD Bioscience).
Annexin 5 apc pi apoptosis kit
Manufactured by Elabscience
Sourced in China
The Annexin V-APC/PI Apoptosis Kit is a laboratory analysis tool used to detect and quantify apoptosis, a form of programmed cell death, in cell samples. It utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a DNA-binding dye, to differentiate between viable, early apoptotic, and late apoptotic/necrotic cells through flow cytometry analysis.
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3 protocols using annexin 5 apc pi apoptosis kit
1
Quantifying Apoptosis by Flow Cytometry
2
Annexin V-based Apoptosis Assay
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Cell apoptosis rates were analyzed using an Annexin V-APC/7-AAD Apoptosis Kit (Elabscience, China) or Annexin V-APC/PI Apoptosis Kit (Elabscience, China) according to the manufacturer’s instructions. Cells were incubated with HHT in 6-well plates and washed twice with cold PBS. Subsequently, cells were incubated with Annexin V-APC and 7-AAD or PI for 20 minutes at room temperature. The percentage of apoptotic cells was measured using Attune NXT flow cytometry (Thermo Fisher Scientific, USA).
3
Apoptosis Analysis of Cells Exposed to ZEN
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Cells were plated on six-well plates and exposed to 10 μg/mL of ZEN for 48 h. Cell samples were washed with PBS and sequentially stained with Annexin V-APC and (PI) using the Annexin V-APC/PI Apoptosis Kit (Elabscience, Wuhan, China). The stained samples were analyzed by flow cytometry and followed by CytExpert 2.3 (Beckman Coulter, Brea, CA, USA) to count cell apoptosis rate.
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