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Byl719

Manufactured by Active Motif
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BYL719 is a lab equipment product. It is a small-molecule inhibitor that selectively targets the PI3Kα isoform.

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Lab products found in correlation

Bkm120, by Active Motif (2 mentions) Bez 235, by Active Motif (1 mentions) Aspirin, by Merck Group (1 mentions) Celecoxib, by Selleck Chemicals (1 mentions) Bafilomycin a, by Merck Group (1 mentions) Sodium salicylate, by Merck Group (1 mentions) Bp231 100, by Thermo Fisher Scientific (1 mentions) Gdc 0941, by Cayman Chemical (1 mentions) Mk 2206, by Cayman Chemical (1 mentions) Gdc0032, by Selleck Chemicals (1 mentions) Gdc 0068, by Selleck Chemicals (1 mentions) Recombinant human c5a, by R&D Systems (1 mentions) Wortmannin, by Merck Group (1 mentions) Tms diazomethane, by Merck Group (1 mentions) Murine tnf α, by R&D Systems (1 mentions) Murine gm csf, by Thermo Fisher Scientific (1 mentions) Fatty acid free bsa, by Merck Group (1 mentions) Hank s balanced salt solution, by Merck Group (1 mentions) M csf, by Thermo Fisher Scientific (1 mentions) Luminol, by Merck Group (1 mentions)

5 protocols using byl719

1

Inhibition of Cellular Pathways

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Inhibitors BKM120 (#A-1108), BEZ235 (#A-1009), BYL719 (#A-1214), and MKK2206 (#A-1206) were purchased from Active Biochem Co. The IKKβ ATP–competitive inhibitor compound A (61 (link)) was manufactured by Bayer Pharmaceuticals.
Thomas C., Henry W., Cuiffo B.G., Collmann A.Y., Marangoni E., Benhamo V., Bhasin M.K., Fan C., Fuhrmann L., Baldwin A.S., Perou C., Vincent-Salomon A., Toker A, & Karnoub A.E. (2017). Pentraxin-3 is a PI3K signaling target that promotes stem cell–like traits in basal-like breast cancers. Science signaling, 10(467), eaah4674.
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2

Characterization of IKKβ Inhibitors

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The IKKβ ATP competitive inhibitor, Compound A was a generous gift from the Baldwin Lab (Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill), and manufactured by Bayer Pharmaceuticals. Celecoxib (#S1261) was purchased from Selleckchem. BKM120 (#A-1108) and BYL719 (#A-1214) were purchased from Active Biochem. A769662-10mg (#ab120335) was purchased from Abcam. Aspirin (#A2093), Sodium salicylate (#A5376) and Bafilomycin A (#B1793) were purchased from Sigma Aldrich. Aspirin/salicylate was prepared as previously described (14 (link)). Briefly, Aspirin was dissolved in 1M Tris-HCl (pH 7.5) to a stock concentration of 1M and final pH of 7.2. An equivalent volume of Tris-HCl (pH 7.2) was used as vehicle control.
Henry W.S., Laszewski T., Tsang T., Beca F., Beck A.H., McAllister S.S, & Toker A. (2016). Aspirin suppresses growth in PI3K-mutant breast cancer by activating AMPK and inhibiting mTORC1 signaling. Cancer research, 77(3), 790-801.
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3

Kinase Inhibitors for Cell Signaling

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The small-molecule kinase inhibitors used included the PI3K catalytic inhibitors GDC-0941 (Cayman Chemical, 11600)44 (link) and GDC-0032 (Selleck Chemicals, S7103)45 (link) and BYL-719 (Active Biochem, A-1214)46 (link), the AKT catalytic inhibitor GDC-0068 (Selleck Chemicals, S2808)47 (link), and the AKT allosteric inhibitor MK-2206 (Cayman Chemical, 1032350-13-2)48 (link). Note that AKT catalytic inhibitors are known to increase AKT phosphorylation on threonine 308 and serine 473 by stabilizing a conformation in which these phosphorylated residues are inaccessible to phosphatases while, at the same time, fully inhibiting AKT kinase activity and phosphorylation of downstream substrates49 (link). Inhibitor stocks were dissolved in DMSO at 10 mM under sterile conditions, aliquoted and stored at −80 °C. Aliquots were freeze–thawed no more than five times. Insulin/growth factor treatments were preceded by 15 min of pretreatment with DMSO (Thermo Fisher Scientific, BP231-100) or a small-molecule inhibitor dissolved in DMSO. The inhibitors or DMSO were also present in the medium during labelling.
Dibble C.C., Barritt S.A., Perry G.E., Lien E.C., Geck R.C., DuBois-Coyne S.E., Bartee D., Zengeya T.T., Cohen E.B., Yuan M., Hopkins B.D., Meier J.L., Clohessy J.G., Asara J.M., Cantley L.C, & Toker A. (2022). PI3K drives the de novo synthesis of coenzyme A from vitamin B5. Nature, 608(7921), 192-198.
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4

Murine Macrophage Signaling Assay

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Bovine FGN, poly-RGD+, fMLP, luminol, TMS-diazomethane, wortmannin, fatty acid–free BSA, and HRP were from Sigma-Aldrich. Murine GM-CSF and M-CSF were from PeproTech, whereas murine TNF-α and recombinant human C5a were from R&D Systems. SRBCs in Alsever’s were from TCS Biosciences. Hanks’ balanced salt solution and Dulbecco’s phosphate-buffered saline (dPBS) with Ca2+ and Mg2+ were from Sigma. The isoform-selective PI3K inhibitors IC87114 and TGX221 were from Calbiochem, whereas BYL719 was from Active Biochem. All tissue culture products were from Invitrogen. All buffer components were from Sigma-Aldrich and were endotoxin-free or low-endotoxin, as available. Internal standards for lipid analysis, 1-heptadecanoyl-2-hexadecanoyl-sn-glycero-3-(phosphoinositol 3,4,5-trisphosphate) (C17:0/C16:0-PIP3, as a hepta sodium salt), and C17:0/C16:0-PI were synthesized at the Babraham Institute. All chemicals and solutions were of analytical reagent grade.
Houslay D.M., Anderson K.E., Chessa T., Kulkarni S., Fritsch R., Downward J., Backer J.M., Stephens L.R, & Hawkins P.T. (2016). Coincident signals from GPCRs and receptor tyrosine kinases are uniquely transduced by PI3Kβ in myeloid cells. Science signaling, 9(441), ra82.
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5

Chemical Compounds for Cell Signaling

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The following chemicals were purchased from Sigma Aldrich: iron chloride hexahydrate, iron citrate, ammonium iron citrate, deferoxamine mesylate salt, erastin L-glutamate, arachidonic acid, aminooxyacetic acid, hydrogen peroxide, and carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The EMD kinase library, PI3Kγ/CKII Inhibitor, PI3Kβ Inhibitor VI, PI3Kγ Inhibitor VII, PI3Kα Inhibitor IV, PI3Kα Inhibitor VIII, and Flt3 Inhibitors I, II, III were purchased from EMD Bioscience. GDC0032 and BYL-719 were purchased from Active Biochem. Quizartinib was purchased from Selleckchem.
Kang Y., Tiziani S., Park G., Kaul M, & Paternostro G. (2014). Cellular Protection using Flt3 and PI3Kα inhibitors demonstrates multiple mechanisms of oxidative glutamate toxicity. Nature communications, 5, 3672.
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