Paladur acrylic dental cement

Rats were anesthetized by isoflurane inhalation (4%, for 4 min) in an induction chamber and maintained afterward with 2% isoflurane with a flow of 4 liters/min. Stereotactic surgery was performed as previously described (24 (link), 38 (link), 78 (link)). Briefly, rats were mounted on a stereotactic frame (Kopf Instruments, Tujunga, CA, USA), an incision was made along the midline of the skull, the periosteum was removed, and small holes were drilled for the implantation of guide cannulae (Invivo1, Roanoke, VA, USA). Coordinates were taken from the Paxinos and Watson brain atlas, relative to bregma, as follows: anterior-posterior: −5.8, mediolateral: ±2.2, dorsoventral: −6.45. VTA guide cannulae were inserted at an angle of 11^o. For microdialysis, a CMA 12 guide cannula (CMA Microdialysis AB, Kita, Sweden) was implanted unilaterally in the NAc in the following coordinates, relative to bregma: anterior-posterior: +1.2, mediolateral: −1.5, dorsoventral: −6.5. NAc guide cannulae were inserted at an angle of 0^o. Cannulae were fixated on the skull with three anchoring screws and Paladur acrylic dental cement (Kulzer, Hanau, Germany). Correct cannula placement was confirmed in the end of experiments.