Lipopolysaccharide lps derived from escherichia coli serotype 0111 b4

β-actin-SRG3 Tg C57BL/6 (B6) and CD2-SRG3 Tg B6 mice were backcrossed with MBP TCR Tg B10.PL mice more than eleven and seven times, respectively. MBP TCR Tg B10.PL mice [23 (link)] were provided from Dr. Luc Van Kaer (Vanderbilt University, Nashville, USA) by kind permission of Dr. Juan J. Lafaille (Rockefeller University, USA). The mice were bred and maintained at Sejong University and were 6–12 weeks old in the experiments performed, unless otherwise specified. The mice were fed a γ-irradiated sterile diet and autoclaved distilled water. The animal experiments were approved by the Institutional Animal Care and Use Committee at Sejong University (SJ-20100401009). TLR ligands were used as positive controls. Lipopolysaccharide (LPS) derived from Escherichia coli (serotype 0111:B4) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Recombinant murine IL3 was purchased from R&D systems (Minneapolis, MN, USA).

TRIzol was obtained from Invitrogen™ (Thermo Fisher Scientific, Inc., Waltham, MA, USA). QiaQuick PCR extraction kit was obtained from Qiagen (Venlo, The Netherlands). PrimeScript^TM 1st Strand cDNA Synthesis Kit and SYBR^® Premix Ex Taq TM II kit were purchased from TaKaRa (Dalian, China). Lipopolysaccharide (LPS) derived from Escherichia coli (serotype 0111:B4) was acquired from Sigma (St. Louis, MO, USA). All other reagents were purchased from commercial sources.

Wild type (WT) C57BL/6 (B6) mice were purchased from Jung Ang Lab Animal Inc. (Seoul, Korea). CD1d KO B6 mice were provided by Dr. A. Bendelac (University of Chicago, IL, USA). Foxp3(GFP) WT B6 mice were obtained from Dr. Rho H. Seong (Seoul National University, Seoul, Korea). Foxp3(GFP) WT B6 mice were further crossed with CD1d KO B6 mice to obtain Foxp3(GFP) CD1d KO B6 mice. DO11.10 OVA-specific TCR transgenic (Tg) Balb/c × B6 F1 mice were generated by intercrossing DO11.10 Balb/c mice and B6 mice. All mice used in this study were maintained at Sejong University and used for experiments at 6-12 weeks of age. Mice were maintained on a 12-hour light/12-hour dark cycle in a temperature-controlled barrier facility with free access to food and water. Mice were fed a γ-irradiated sterile diet and provided with autoclaved tap water. Age- and sex-matched mice were used for all experiments. The animal experiments were approved by the Institutional Animal Care and Use Committee at Sejong University (protocol code SJ-20180804, approved on 4 August 2018). Lipopolysaccharide (LPS) derived from Escherichia coli (serotype 0111:B4) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Ovalbumin (OVA) peptide_323–339 (ISQAVHAAHAEINEAGR) was synthesized by Peptron Inc. (Daejeon, Korea).