Wallac universal gamma counter 1282

Manufactured by PerkinElmer

Sourced in United States

The Wallac Universal Gamma Counter 1282 is a versatile instrument used for the detection and quantification of gamma-emitting radioactive samples. It provides accurate and reliable measurements across a wide range of applications.

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4 protocols using wallac universal gamma counter 1282

Biodistribution of 18F-Labeled Tracers

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Healthy Wistar rats (218–244
g) were injected with 50 ± 2 MBq of either [¹⁸F]1 or [¹⁸F]2 in the tail vein under
isoflurane anesthesia (2% in O₂ at 1 L·min^–1). Rats were conscious for the allowed time between injection and
sacrificing, except for the animals of the 5 min time point, which
were left unconscious for the whole time. Animals were sacrificed
under isoflurane anesthesia at time points 5, 15, or 60 min (n = 3). Blood was collected via a heart puncture from each
rat. Heart, lungs, liver, kidneys, bone, cerebral cortex, cerebellum,
and the rest of the brain were collected, weighed, and counted for
radioactivity in a Wallac Universal Gamma Counter 1282 (PerkinElmer,
Waltham, MA, USA). Biodistribution data were expressed as percentage
injected dose per gram tissue (%ID/g).

Raaphorst R.M., Luurtsema G., Schuit R.C., Kooijman E.J., Elsinga P.H., Lammertsma A.A, & Windhorst A.D. (2017). Synthesis and Evaluation of New Fluorine-18 Labeled Verapamil Analogs To Investigate the Function of P-Glycoprotein in the Blood–Brain Barrier. ACS Chemical Neuroscience, 8(9), 1925-1936.

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Biodistribution of Radiotracer in Wistar Rats

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Healthy male
Wistar rats (200–250 g) were injected with the radiotracer
(25.8 ± 7.6 MBq; 0.74 ± 0.21 nmol; n =
16) via the tail vein under isoflurane anesthesia (2–2.5% in
1 L·min^–1). Animals were sacrificed and dissected
at 5, 15, 30, and 60 min (n = 4 per time point) postinjection.
Blood, heart, lungs, liver, kidney, duodenum, urine, tail, bone, spleen,
large brain, cerebellum, brain stem, spinal cord top, and spinal cord
mid were collected, weighed, and counted for radioactivity in a Wallac
Computagama 1210 or a Wallac Universal Gamma Counter 1282 (Wallac,
PerkinElmer, Waltham, MA, USA). Biodistribution data are expressed
as the percentage of injected dose per gram (%ID/g). Errors are standard
error of the mean (SEM).

Pekošak A., Bulc J.Ž., Korat Š., Schuit R.C., Kooijman E., Vos R., Rongen M., Verlaan M., Takkenkamp K., Beaino W., Poot A.J, & Windhorst A.D. (2018). Synthesis and Preclinical Evaluation of the First Carbon-11 Labeled PET Tracers Targeting Substance P1–7. Molecular Pharmaceutics, 15(11), 4872-4883.

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Biodistribution of [11C]5 in Rats

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Healthy male Wistar rats (210-275 g, 6-8 weeks old, obtained from Harlan) were injected with 30 ± 13 MBq (at start of the experiment) of [¹¹C]5 (corresponds with 0.24 ± 0.07 µg of non-labeled 5) in the tail vein under isoflurane anesthesia. Rats were sacrificed and dissected at 5 and 15 min post injection (n = 4 for 5 min, n = 3 for 15 min). Blood, heart, lungs, liver, kidneys, spleen and brain were collected, weighed and counted for radioactivity in a Wallac Universal Gammacounter 1282 (PerkinElmer). Biodistribution data were expressed as percentage injected dose per gram tissue (%ID/g).

Villa A., Klein B., Janssen B., Pedragosa J., Pepe G., Zinnhardt B., Vugts D.J., Gelosa P., Sironi L., Beaino W., Damont A., Dollé F., Jego B., Winkeler A., Ory D., Solin O., Vercouillie J., Funke U., Laner-Plamberger S., Blomster L.V., Christophersen P., Vegeto E., Aigner L., Jacobs A., Planas A.M., Maggi A, & Windhorst A.D. (2018). Identification of new molecular targets for PET imaging of the microglial anti-inflammatory activation state. Theranostics, 8(19), 5400-5418.

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Lipophilicity Determination of [11C]5

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The distribution of [¹¹C]5 between 1-octanol and 0.2 M phosphate buffer (pH = 7.4) was measured at room temperature. Briefly, 1 mL of a 20 MBq/mL solution of [¹¹C]5 in 0.2 M phosphate buffer (pH = 7.4) was mixed with 1 mL of 1-octanol for 1 min using a vortex mixer. After a settling period of 30 min, three samples of 100 μL were taken from both layers. To determine the recovery (> 97%), three samples of 100 μL were taken from the 20 MBq/mL solution. All samples were counted for radioactivity Wallac Universal Gammacounter 1282 (PerkinElmer). The LogD_oct,7.4 value was calculated according to LogD_oct,7.4 = ¹⁰Log(A_oct/A_buffer), where A_oct and A_buffer represent average radioactivity counts of the three 1-octanol and the three buffer samples. Results are presented as mean ± standard deviation (SD).

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