Ethyl 3 3 dimethylaminopropyl carbodiimide edc

Carboxyl-functionalized magnetic polystyrene microspheres (MPMs) were purchased from Beisile Co., Ltd. (Tianjin, China). N-hydroxysuccinimide (NHS) and ethyl-3-[3-(dimethylamino)propyl] carbodiimide (EDC) were purchased from Sigma-Aldrich Co., Ltd. (Shanghai, China). The BCA protein quanti cation kit was obtained from Solarbio Science and Technology Co., Ltd. (Beijing, China). Chloroauric acid was purchased from Sigma-Aldrich Co., Ltd. (Shanghai, China). White sesame seeds were purchased from Tianjin, China. Sesame allergen monoclonal antibody was prepared in our laboratory. CTAB, AA, Na 2 S 2 O 3 , NaCl, KCl, CaCl 2 , and MgSO 4 were purchased from Sinopharm Group Co., Ltd. (Tianjin, China). The commercial sesame allergen ELISA kit was purchased from Preibang Co., Ltd. (Shandong, China). β-Lactoglobulin was purchased from Jiangtian Chemical Co., Ltd. (Tianjin, China). Peanut protein, walnut protein, casein, and lupin protein were prepared in our laboratory. Bread, biscuits, almond beverage, and energy bars were purchased from a supermarket from Tianjin, China.
A UV spectrophotometer and microplate reader were used to detect the absorbance of gold nanoparticles.
The TEM images of prepared AuNPs were obtained by a transmission electron microscope (TEM, FEI Tecnai F20 SuperTwinFEG, USA, https://www.fei.com/).