Rd291001200r

Plasma metabolic parameters, including the levels of triglyceride, Cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), Alkaline phosphatase (ALKP), vascular endothelial growth factor (VEGF), tumor necrosis factor-α (TNF-α)(R&D Systems, Minneapolis, MN), and leptin (Biovendor RD291001200R, Brno, Czech Republic) were determined by enzyme-linked immunosorbent assays according to the manufacturers’ instructions.

The following plasma metabolites and enzymes were measured: basal glucose, triglycerides, total cholesterol, high-density lipoprotein (HDL), urea, bilirubin, alkaline phosphatase (ALKP), and the hepatic enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyl transpeptidase (γGT). These metabolites were analyzed using commercial kits according to the manufacturer's instructions and a Hitachi 737 Automatic Analyzer (Hitachi Ltd, Tokyo, Japan). Very low-density lipoprotein (VLDL) were estimated following the Friedewald equation (Warnick et al., 1990 (link)) and low-density lipoprotein (LDL) was determined by the modification of Friedewal equation proposed by Ahmadi et al. (2008 (link)): VLDL = TG/5; LDL = [(TChol/1.19)+(TG/1.9)−(HDL/1.1)−38]. The plasma levels of leptin were measured using a commercial rat leptin ELISA kit (Cat. no. RD291001200R; BioVendor, Brno, Czech Republic).

Blood samples were collected at harvest from Apoe^−/−, Apoe^−/−ΔdblGATA, and Apoe^−/−ΔdblGATA mice treated with mEar1 or received adoptive transfer of eosinophils from WT, Il4^−/−, and Il13^−/− mice. Plasma samples were stored at −80°C until analysis. Plasma total cholesterol (23-666-200, Pointe Scientific, Canton, MI), triglyceride (23-666-410, Pointe Scientific), and HDL (23-666-301, Pointe Scientific) levels were determined using the enzymatic methods according to the manufacturer. The LDL cholesterol level was determined using the Friedewald formula: Plasma LDL cholesterol concentration (mg/dL) = total cholesterol−HDL cholesterol−(triglycerides/5). Plasma BMP-2 (MBS262739, MyBioSource, San Diego, CA), BMP-4 (MBS260174, MyBioSource), mEar1 (SK00128-03, Aviscera Bioscience), leptin (RD291001200R, Biovendor, Asheville, NC), IL-1β, IL-6, TNF-α, IFN-γ (88-7013, 88-7064, BMS607-3TWO, 88-7314, Thermo Fisher Scientific) levels were determined using ELISA kits according to the manufacturers’ instructions.