1450ep scanning electron microscope

Manufactured by Zeiss

The Zeiss 1450EP scanning electron microscope is a high-performance laboratory instrument designed for detailed analysis of microscopic samples. It provides high-resolution imaging capabilities and is capable of producing detailed, high-quality images of the surface topography and composition of a wide range of materials.

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Autosamdri 814 critical point dryer, by Tousimis (1 mentions)

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Scanning electron microscope

2 protocols using 1450ep scanning electron microscope

Microstructural Analysis of Chicken Palate

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The palate and base of the oral cavity of P3 male-line female chickens were dissected and fixed in SEM fixative containing 4% PFA, 2.5% glutaraldehyde in 0.1 M PBS over 48 hr at room temperature. The tissue was trimmed and thoroughly rinsed in 0.1 M PBS and processed further into a series of 1% osmium tetroxide (OsO₄), 1% tannic acid and 1% OsO₄ aqueous solutions for 1 hr on ice. The tissues were dehydrated sequentially with ethanol (35%, 50%, 70%, 95% and 100%; three times at each concentration for 2 hr each). Specimens were dried completely using a critical point dryer (Autosamdri-814 Critical Point Dryer, Tousimis Research Corporation, Rockville, MD, USA). The samples were mounted onto SEM stub, sputter coated with gold and photomicrographs were taken using a Zeiss 1450EP scanning electron microscope (Carl Zeiss MicroImaging, Inc., NY, and Oxford Instruments X-Ray Technology, Inc., CA).

Rajapaksha P., Wang Z., Venkatesan N., Tehrani K.F., Payne J., Swetenburg R.L., Kawabata F., Tabata S., Mortensen L.J., Stice S.L., Beckstead R, & Liu H.X. (2016). Labeling and analysis of chicken taste buds using molecular markers in oral epithelial sheets. Scientific Reports, 6, 37247.

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Analyzing HS Hydrogel Microarchitecture via SEM

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The microarchitecture of HS hydrogels was observed using a Zeiss 1450EP scanning electron microscope (Carl Zeiss). Sulfated-HS hydrogels (3 wt %, final concentration of DIBO-functionalized 4-armed PEG polymer, 17) were prepared in a polydimethysiloxane (PDMS) mold (16 mm diameter and 3 mm thickness), swelled in PBS for 24 h at 37 °C, flash frozen in liquid nitrogen, and then lyophilized for 48 h. The lyophilized gels were mounted on 10 mm SEM stubs and sputter coated (Structure Probe Inc.) with gold for 60 s before being imaged under an accelerated voltage of 20 kV. Images were acquired at 1000× and 1500× magnifications to observe the porosity and structure of the hydrogels. For pore size analysis, 1000× magnified images were processed using ImageJ software (NIH) (Figure 1C).

Chopra P., Logun M.T., White E.M., Lu W., Locklin J., Karumbaiah L, & Boons G.J. (2019). Fully Synthetic Heparan Sulfate-Based Neural Tissue Construct That Maintains the Undifferentiated State of Neural Stem Cells. ACS chemical biology, 14(9), 1921-1929.

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