Cell culture slides

Manufactured by Corning

Sourced in United States

Corning Cell Culture Slides are designed for in vitro cell culture studies. The slides provide a flat, sterile surface for the growth and observation of adherent cells.

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Lab products found in correlation

Lsm 780, by Zeiss (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Paraformaldehyde solution, by Santa Cruz Biotechnology (2 mentions) Vybrant cm dil solution, by Thermo Fisher Scientific (2 mentions) F actin alexa fluor 488 phalloidin, by Thermo Fisher Scientific (2 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

Spelling variants of this product

Culture slides (32 citations) Falcon™ Chambered Cell Culture Slides (4 citations) 4-well cell culture chamber slides (2 citations)

3 protocols using cell culture slides

Exosome Uptake in Stem Cells

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For the cellular uptake of exosomes (hTERT MSC Exosomes, ATCC, USA), both ASCs and NSCs were seeded with 500 cells per well on the Cell Culture Slides (Corning®, USA). After 3 days cultured in DMEM containing 10% FBS and 1% AA. Before cellular uptake, the concentration, size distribution, and diameter of exosomes were detected by the Nanosight system (NS300, Malvern, UK). The particles concentration and size distribution were measured by nanoparticle tracking analysis (v3.20 software). All exosomes were labeled with 1 mM Vybrant® CM-Dil solution (Invitrogen, USA). Then, the CM-Dil labeled exosomes were incubated with both ASCs and NSCs in serum-free DMEM at 37 °C for 0, 1, 2, 3, 4 h, respectively. After fixed with 4% paraformaldehyde solution (Santa Cruz, USA) and 5% BSA (Sigma, USA), the cells were stained with F-actin Alexa Fluor™ 488 Phalloidin (Invitrogen, A12379, USA) and Hoechst 33,342 (Thermo, USA) respectively. Finally, the fluorescent images were observed and captured using confocal laser scanning microscopy (LSM 780, Zeiss, Germany). All red area was imported into Image J (v1.48) for quantization.

Shi G., Hao D., Zhang L., Qin J., Tian G., Ma B, & Zhou X. (2021). Endocytosis-associated patterns in nerve regeneration after peripheral nerve injury. Journal of Orthopaedic Translation, 31, 10-19.

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Characterizing TEPEP Patch Release and Tenocyte Uptake

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The release ratio of TEPEP patch was tested using a NanoSight System (NS300, Malvern, UK). Briefly, 20% TEPEP patch was mixed with 3 ml PBS, following incubation in a 15 ml tube at 37 °C. After incubating in a 37 °C incubator for 1 day, the supernatant PBS was collected and moved to another 6 ml collecting tube for testing. Add the new PBS and repeat until day 14. The particle concentration and size distribution was measured by nanoparticle tracking analysis (v3.20 software).
For the cellular uptake of PEP, tenocytes were harvested from fresh FDP tendons from Mixed-breed dogs mentioned above based on well-established protocol.¹⁶ Tenocytes (P4) were seeded with 300 cells per well on a Cell Culture Slides (Corning®, USA). After 2 days cultured in DMEM containing 10% FBS and 1% AA. The PEP was labeled with 1 mM Vybrant® CM-Dil solution (Invitrogen, USA). Then, the CM-Dil labeled PEP was incubated with tenocytes in serum-free DMEM at 37 °C for 2 h. After washed with PBS and fixed in 4% paraformaldehyde solution (Santa Cruz, USA), the tenocytes were stained with F-actin Alexa Fluor™ 488 Phalloidin (Invitrogen, A12379, USA) and Hoechst 33342 (Thermo, USA) respectively. Finally, the fluorescent images were observed and captured using confocal laser scanning microscopy (LSM 780, Zeiss, Germany).

Shi G., Wang Y., Wang Z., Thoreson A.R., Jacobson D.S., Amadio P.C., Behfar A., Moran S.L, & Zhao C. (2020). A novel engineered purified exosome product patch for tendon healing: An explant in an ex vivo model. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 39(8), 1825-1837.

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Tracking Exosome Uptake in Macrophages

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Osteosarcoma and fibroblast exosomes were labeled with Cell Tracker CM-DiI red dye (Invitrogen, C7000). Briefly, exosomes were incubated with 1 micromole of dye at 37°C for 5 min. Exosomes were then incubated at 4°C for 15 min. The labeled exosomes were diluted in 35 mL of PBS and subjected to ultracentrifugation at 100,000 × g at 4°C for 2 h. The exosome pellet was washed in 35 mL of PBS and a second ultracentrifugation was performed at 100,000 × g at 4°C for 2 h. Next, the exosome pellet was resuspended in 210 μL of PBS. MHS cells were plated on cell culture slides (Corning, 53106–304) and treated with labeled osteosarcoma or fibroblast exosomes. The slides were imaged after 24 h using the Nikon Eclipse Ti de-convolution inverted bright field and fluorescent microscope (Nikon Instruments, Melville, New York). PBS treated MHS cells were used as control.

Wolf-Dennen K., Gordon N, & Kleinerman E.S. (2020). Exosomal communication by metastatic osteosarcoma cells modulates alveolar macrophages to an M2 tumor-promoting phenotype and inhibits tumoricidal functions. Oncoimmunology, 9(1), 1747677.

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