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Media and sera

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Media and sera are essential components for cell culture and tissue culture applications. They provide the necessary nutrients, growth factors, and other essential elements to support the growth and maintenance of cells in vitro. These products are designed to meet the specific requirements of various cell types and research applications.

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Lab products found in correlation

Glucose and amino acid isotope tracers, by Cambridge Isotopes (4 mentions) Sphingolipid standards, by Avanti Polar Lipids (3 mentions) Fatty acid standards, by Larodan (2 mentions) Isotope tracers, by Cambridge Isotopes (1 mentions) Fenofibrate, by Merck Group (1 mentions) Uk5099, by Merck Group (1 mentions)

7 protocols using media and sera

1

Modulating Lipid Metabolism in Cells

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Media and sera were purchased from Life Technologies. Isotope tracers were purchased from Cambridge Isotopes. Sphingolipid standards were purchased from Avanti Polar Lipids. All other reagents were purchased from Sigma unless otherwise noted.
Cells were treated at a concentration of 5 μM UK5099 (catalog no.: PZ0160; MilliporeSigma), 10 nM Myr (catalog no.: M1177; MilliporeSigma), 10 μM FuB1 (catalog no.: F1147; MilliporeSigma), or 20 μM Fenofibrate (catalog no.: PHR1246; Sigma) with stock solutions prepared in DMSO. DeoxySA m18:0 (catalog no.: 860493P; Avanti) and amino acids were supplemented to growth media with concentrations as indicated in the text.
Cordes T., Kuna R.S., McGregor G.H., Khare S.V., Gengatharan J., Muthusamy T, & Metallo C.M. (2022). 1-Deoxysphingolipid synthesis compromises anchorage-independent growth and plasma membrane endocytosis in cancer cells. Journal of Lipid Research, 63(10), 100281.
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2

Isotope Tracing for Metabolism

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Media and sera were purchased from Life Technologies. Glucose and amino acid isotope tracers were purchased from Cambridge Isotopes Inc. Fatty acid standards were purchased from Larodan. All other reagents were purchased form Sigma-Aldrich unless otherwise noted.
Wallace M., Green C.R., Roberts L.S., Lee Y.M., McCarville J.L., Sanchez-Gurmaches J., Meurs N., Gengatharan J.M., Hover J.D., Phillips S.A., Ciaraldi T.P., Guertin D.A., Cabrales P., Ayres J.S., Nomura D.K., Loomba R, & Metallo C.M. (2018). Enzyme promiscuity drives branched-chain fatty acid synthesis in adipose tissues. Nature chemical biology, 14(11), 1021-1031.
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3

Isotope Tracer Reagent Protocol

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Media and sera were purchased from Life Technologies (Carlsbad, CA, USA) unless otherwise stated. Glucose and amino acid isotope tracers were purchased from Cambridge isotopes Inc. All other reagents were purchased from Sigma Aldrich (St. Louis, MO, USA) unless otherwise stated.
Cordes T, & Metallo C.M. (2021). Itaconate Alters Succinate and Coenzyme A Metabolism via Inhibition of Mitochondrial Complex II and Methylmalonyl-CoA Mutase. Metabolites, 11(2), 117.
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4

Metabolic Isotope Labeling Protocol

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Media and sera were purchased from Life Technologies. Glucose and amino acid isotope tracers were purchased from Cambridge Isotopes Inc. Sphingolipid standards were purchased from Avanti polar lipids. All other reagents were purchased from Sigma-Aldrich unless otherwise noted.
Muthusamy T., Cordes T., Handzlik M.K., You L., Lim E.W., Gengatharan J., Pinto A.F., Badur M.G., Kolar M.J., Wallace M., Saghatelian A, & Metallo C.M. (2020). Serine restriction alters sphingolipid diversity to constrain tumor growth. Nature, 586(7831), 790-795.
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5

AMG-510 Sotorasib Protocol

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Media and sera were purchased from Life Technologies and R&D Systems, respectively. AMG-510 (sotorasib) was acquired from Selleck Chemicals (catalog no. S8830). All other reagents were from Sigma-Aldrich unless otherwise noted.
Stein B.D., Ferrarone J.R., Gardner E.E., Chang J.W., Wu D., Hollstein P.E., Liang R.J., Yuan M., Chen Q., Coukos J.S., Sindelar M., Ngo B., Gross S.S., Shaw R.J., Zhang C., Asara J.M., Moellering R.E., Varmus H, & Cantley L.C. (2023). LKB1-Dependent Regulation of TPI1 Creates a Divergent Metabolic Liability between Human and Mouse Lung Adenocarcinoma. Cancer Discovery, 13(4), 1002-1025.
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6

Metabolic Isotope Labeling Protocol

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Media and sera were purchased from Life Technologies. Glucose and amino acid isotope tracers were purchased from Cambridge Isotopes Inc. Sphingolipid standards were purchased from Avanti polar lipids. All other reagents were purchased from Sigma-Aldrich unless otherwise noted.
Muthusamy T., Cordes T., Handzlik M.K., You L., Lim E.W., Gengatharan J., Pinto A.F., Badur M.G., Kolar M.J., Wallace M., Saghatelian A, & Metallo C.M. (2020). Serine restriction alters sphingolipid diversity to constrain tumor growth. Nature, 586(7831), 790-795.
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7

Isotope Tracing for Metabolism

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Media and sera were purchased from Life Technologies. Glucose and amino acid isotope tracers were purchased from Cambridge Isotopes Inc. Fatty acid standards were purchased from Larodan. All other reagents were purchased form Sigma-Aldrich unless otherwise noted.
Wallace M., Green C.R., Roberts L.S., Lee Y.M., McCarville J.L., Sanchez-Gurmaches J., Meurs N., Gengatharan J.M., Hover J.D., Phillips S.A., Ciaraldi T.P., Guertin D.A., Cabrales P., Ayres J.S., Nomura D.K., Loomba R, & Metallo C.M. (2018). Enzyme promiscuity drives branched-chain fatty acid synthesis in adipose tissues. Nature chemical biology, 14(11), 1021-1031.
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