High ph buffer

Manufactured by Leica

High-pH buffer is a laboratory solution designed to maintain a specific pH range, typically above 7.0. It is used to create and maintain an alkaline environment for various experimental procedures and applications. The core function of this buffer is to provide a stable, controlled pH environment for experiments that require a high pH level.

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Lab products found in correlation

Bond max, by Leica (1 mentions) Ny eso 1, by Thermo Fisher Scientific (1 mentions)

2 protocols using high ph buffer

IHC Analysis of p53 Expression

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Immunohistochemistry (IHC) analysis of p53 expression was performed on 2 µm sections mounted on glass slides in a Leica Bond‐Max automated immunostainer (Leica Microsystems Inc., Bannockburn, Ireland). Heat‐induced epitope retrieval was performed with high‐pH buffer (Leica) for 20 min. The p53 specific antibody DO7 (Leica Biosystems, Wetzlar, Germany) was diluted 1:100 and incubated for 30 min, followed by the Leica polymer (15 min). Diaminobenzidine was used as the chromogen with subsequent light haematoxylin counterstain.

Wegert J., Vokuhl C., Ziegler B., Ernestus K., Leuschner I., Furtwängler R., Graf N, & Gessler M. (2017). TP53 alterations in Wilms tumour represent progression events with strong intratumour heterogeneity that are closely linked but not limited to anaplasia. The Journal of Pathology: Clinical Research, 3(4), 234-248.

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Automated IHC Protocol for NY-ESO-1 and MAGE-A

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Most cases were studied by automated IHC using Leica Bond Max automation with Leica refine detection kit. The primary antibodies used were NY-ESO-1 (1:100 dilution; Invitrogen/Life Technologies, Grand Island, NY) and MAGE-A (1:100 dilution; Santa-Cruz Biotech, Dallas, TX). Heat-induced epitope retrieval was used for 25 minutes (Leica high-pH buffer), followed by incubation of primary antibody at room temperature (30 min). This was followed by Leica polymer (15 min), postpolymer (15 min), peroxide block, diaminobenzidine (10 min), and hematoxylin counterstain. A small number of cases were studied using Ventana Benchmark Ultra automation with the same primary antibodies and reagents associated with that automation, including high pH epitope retrieval buffer.
NY-ESO-1 and MAGE staining intensity was assessed by at least 2 pathologists and was scored as positive or negative depending on the clear presence of tumor cell staining that was cytoplasmic for NY-ESO and cytoplasmic and/or nuclear for MAGE-A. Intensity of staining was also assessed and both weak and strong intense staining was considered positive. The percentage of staining neoplastic cells was estimated for each case and sections showing ≥ 50% of the tumor cells staining positive with any intensity were categorized as positive for our study.

Park T.S., Groh E.M., Patel K., Kerkar S.P., Lee C.C, & Rosenberg S.A. (2016). Expression of MAGE-A and NY-ESO-1 in Primary and Metastatic Cancers. Journal of immunotherapy (Hagerstown, Md. : 1997), 39(1), 1-7.

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