Unity inova 800 nmr spectrometer

The optical rotations were obtained using a Jasco P-1020 polarimeter (Jasco, USA). The nuclear magnetic resonance (NMR) spectra were obtained using a Varian UNITY INOVA 800 NMR spectrometer operating at 800 MHz (¹H) and 200 MHz (¹³C), with chemical shifts given in ppm (δ). Preparative HPLC was performed using a Waters 1525 Binary HPLC pump with a Waters 996 Photodiode Array Detector (Waters Corporation, USA). Semi-preparative HPLC was conducted using a Shimadzu Prominence HPLC System with SPD-20A/20AV Series Prominence HPLC UV-Vis Detectors (Shimadzu, Japan). LC/MS analysis was carried out on an Agilent 1200 Series HPLC System (Agilent Technologies, USA) equipped with a diode array detector and a 6130 Series ESI mass spectrometer by using an analytical Kinetex column (4.6 × 100 mm, 3.5 μm). Precoated Merck silica gel F254 plates and RP-18 F254s plates were used for thin-layer chromatography (TLC). Spots were detected by TLC under UV light or by heating after spraying with anisaldehyde-sulfuric acid.

UV spectra were acquired using Agilent 8453 UV–visible spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). NMR spectra were acquired using Varian UNITY INOVA 800 NMR spectrometer (Varian, Palo Alto, CA, USA) operating at 800 MHz (¹H) with chemical shifts reported in ppm (δ). Preparative high-performance liquid chromatography (HPLC) was performed using Waters 1525 Binary HPLC pump equipped with Waters 996 Photodiode Array Detector (Waters Corporation, Milford, CT, USA). Diaion HP-20 (Mitsubishi Chemical, Tokyo, Japan) was used for open-column chromatography. Semi-preparative HPLC was performed using Shimadzu Prominence HPLC System equipped with SPD-20A/20AV Series Prominence HPLC UV–Vis Detector (Shimadzu, Tokyo, Japan). LC/MS analyses were performed using Agilent 1200 Series HPLC System (Agilent Technologies, Santa Clara, CA, USA) equipped with a diode array detector and 6130 Series ESI mass spectrometer with an analytical Kinetex HPLC column (4.6 × 100 mm, 3.5 μm). Merck pre-coated silica gel F254 plates and RP-18 F254s plates were used for thin-layer chromatography (TLC). Spots were detected on TLC plates under UV light or by heating after spraying with anisaldehyde sulfuric acid.