Log In Sign up
The largest database of trusted experimental protocols

Wallac 1480

Manufactured by PerkinElmer
Visit Supplier

The Wallac 1480 is a compact and versatile liquid scintillation counter designed for accurate and reliable radioactivity detection. It features a high-performance photomultiplier tube and advanced signal processing technology to provide precise measurements of various radioisotopes. The Wallac 1480 is capable of performing a wide range of applications in research and clinical settings.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Wallac 1480 automated gamma counter (3 citations) Wallac Wizard 1480 Automatic Gamma Counter (4 citations) Wallac Wizard 3″ 1480 (11 citations) Wallac Wizard 1480 (54 citations) Wallac Wizard 1480-011 Automatic Gamma Counter (2 citations) Wallac Wizard 1480 Wizard (2 citations) Wallac 1480 Wizard3 Gamma Counter (2 citations) Wallac Wizard 3″ 1480 Automatic Gamma Counter (2 citations) 1480 Wallac Gamma Counter (3 citations)

4 protocols using wallac 1480

1

Specific Binding Assay of Melanoma-Targeting Radiotracers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Specific binding of Al18F-NOTA-PEG2Nle-CycMSHhex (peak 1 and peak 2) and Al18F-NOTA-AocNle-CycMSHhex was determined on B16/F10 cells seeded on 24-well plates. The B16/F10 melanoma cells (1 × 106 cells per well, n = 3) were incubated at 25 °C for 1 h with approximately 11.1 KBq of each isomer of Al18F-NOTA-PEG2Nle-CycMSHhex and Al18F-NOTA-AocNle-CycMSHhex with or without 10 μg (6.07 nmol) of unlabeled [Nle4, D-Phe7]-α-MSH (NDP-MSH) in 0.3 mL of binding medium {Dulbecco’s modified Eagle’s medium with 25 mM N-(2-hydroxyethyl)-piperazine-N’-(2-ethanesulfonic acid), pH 7.4, 0.2% bovine serum albumin (BSA), 0.3 mM 1,10-phenathroline}. The binding medium was aspirated after incubation. The cells were washed twice with 0.5 mL of ice-cold 0.01 M phosphate buffered saline (PBS) buffer containing 0.2% BSA (pH = 7.4), and lysed with 0.5 mL of 1 M NaOH for 5 min, collected and measured in a Wallac 1480 automated gamma counter (PerkinElmer, NJ).
Volpe G., Sbaiz L., Avanzini C., Caramello P, & Savoia D. (2001). Genetic Diversity of Pneumocystis carinii Isolated from Human Immunodeficiency Virus-Positive Patients in Turin, Italy. Journal of Clinical Microbiology, 39(8), 2995-2998.
+ Open protocol
+ Expand
2

MC1R Binding Affinity of NOTA/NODAGA-GGNle-CycMSHhex

Check if the same lab product or an alternative is used in the 5 most similar protocols
The MC1R binding affinities of NOTA/NODAGA-GGNle-CycMSHhex were determined on B16/F10 melanoma cells by in vitro competitive receptor binding assay. The receptor binding assay was replicated in triplicate. The B16/F10 cells (0.5×105 cells/well, n = 3) were incubated at room temperature (25 °C) for 2 h with approximately 30,000 counts per minute (cpm) of 125I-(Tyr2)-NDP-MSH in the presence of 10−12 to 10−5 M of the peptide in 0.3 mL of binding medium {Modified Eagle’s medium with 25 mM N-(2-hydroxyethyl)-piperazine-N’-(2-ethanesulfonic acid), pH 7.4, 0.2% bovine serum albumin (BSA), 0.3 mM 1,10-phenathroline}. The binding medium was aspirated after the incubation. The cells were rinsed twice with 0.5 mL of ice-cold pH 7.4, 0.2% BSA/0.01 M phosphate buffered saline (PBS) and lysed in 0.5 mL of 1 N NaOH for 5 min. The cells were harvested and measured in a Wallac 1480 automated gamma counter (PerkinElmer, NJ). The IC50 value was calculated using the Prism software (GraphPad Software, La Jolla, CA, USA).
Qiao Z., Xu J., Gonzalez R, & Miao Y. (2020). Novel [99mTc]-Tricarbonyl-NOTA-Conjugated Lactam-Cyclized Alpha-MSH Peptide with Enhanced Melanoma Uptake and Reduced Renal Uptake. Molecular pharmaceutics, 17(9), 3581-3588.
+ Open protocol
+ Expand
3

Melanoma Cell Binding of Radiolabeled MSH

Check if the same lab product or an alternative is used in the 5 most similar protocols
The specific binding of 68Ga-DOTA-GGNle-CycMSHhex was determined using B16/F10 and M21 melanoma cells. The B16/F10 and M21 cells (1×106 cells/tube, n = 3) were incubated at 37 °C for 2 h with approximately 0.037 MBq of 68Ga-DOTA-GGNle-CycMSHhex with or without 10 μg (6.07 nmol) of unlabeled NDP-MSH in 0.3 mL of binding medium [Modified Eagle’s medium with 25 mM N-(2-hydroxyethyl)-piperazine-N’-(2-ethanesulfonic acid), pH 7.4, 0.2% bovine serum albumin (BSA), 0.3 mM 1,10-phenathroline]. The binding medium was aspirated after the incubation. The cells were rinsed three times with 0.5 mL of ice-cold pH 7.4, 0.2% BSA/0.01 M phosphate buffered saline (PBS) and measured in a PerkinElmer Wallac 1480 automated γ-counter
Yang J., Xu J., Gonzalez R., Lindner T., Kratochwil C, & Miao Y. (2018). 68Ga-DOTA-GGNle-CycMSHhex targets the melanocortin-1 receptor for melanoma imaging. Science translational medicine, 10(466), eaau4445.
+ Open protocol
+ Expand
4

Melanoma Cell MC1R Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The MC1R binding affinities of DOTA-GGNle-CycMSHhex and Cy5.5-GGNle-CycMSHhex were determined on B16/F10 and M21 melanoma cells by in vitro competitive receptor binding assay. The receptor binding assay was replicated in triplicate. B16/F10 and M21 cells (2×105 cells/well, n = 3) were seeded separately in 24-well cell culture plates and incubated at 37 °C overnight. After being washed with binding medium [Modified Eagle’s medium with 25 mM N-(2-hydroxyethyl)-piperazine-N′-(2-ethanesulfonic acid), pH 7.4, 0.2% bovine serum albumin (BSA), 0.3 mM 1,10-phenathroline], the cells were incubated at 37 °C for 2 h with approximately 45,000 counts per minute (cpm) of 125I-(Tyr2)-NDP-MSH in the presence of 10−12 to 10−5 M of the peptide in 0.3 mL of binding medium. The binding medium was aspirated after the incubation. The cells were rinsed twice with 0.5 mL of ice-cold pH 7.4, 0.2% BSA/0.01 M phosphate buffered saline (PBS) and lysed in 0.5 mL of 1 N NaOH for 5 min. The cells were harvested and measured in a PerkinElmer Wallac 1480 automated γ-counter. The IC50 value was calculated using Prism from GraphPad Software.
Yang J., Xu J., Gonzalez R., Lindner T., Kratochwil C, & Miao Y. (2018). 68Ga-DOTA-GGNle-CycMSHhex targets the melanocortin-1 receptor for melanoma imaging. Science translational medicine, 10(466), eaau4445.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!