Quantification of cellular DNA content at 72 h after transfection with control miRNA or #12 (5 nM) was determined by using a cytometer. Briefly, the cells were harvested and fixed with 70% cold ethanol at −20°C overnight. The fixed cells were then washed twice with PBS and resuspended in 100 μL PBS-based propidium iodide solution containing 0.1% Triton X-100 (Wako, Osaka, Japan), 0.2 mg/mL RNase A (Life Technologies), and 20 μg/mL propidium iodide (Life Technologies). Thereafter, they were incubated for 30 min at room temperature protected from the light. The DNA content in the cells was analyzed with a cytometer (The Tail Image-Based Cytometer, Life Technologies).
Tail image based cytometer
Manufactured by Thermo Fisher Scientific
The Tail Image-Based Cytometer is a laboratory instrument designed to capture and analyze images of cells or particles. It utilizes advanced imaging technology to provide detailed information about the physical and morphological characteristics of the sample.
Automatically generated - may contain errors
2 protocols using tail image based cytometer
1
Cellular DNA Content Quantification
2
Cellular DNA Content Quantification
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Quantification of cellular DNA content at 48 h after treatment with CRC-EVs was determined by using a cytometer. Briefly, the cells were harvested and fixed with 70% cold ethanol at -20°C overnight. The fixed cells were washed twice with PBS, resuspended in 100 μl PBS-based propidium iodide solution containing 0.1% Triton X-100 (Wako, Osaka, Japan), 0.2 mg/ml RNase A (Life Technologies), and 20 μg/ml propidium iodide (Life Technologies), and incubated for 30 min at room temperature protected from the light. The DNA content in the cells was analyzed through the cytometer (The Tail® Image-Based Cytometer, Life Technologies).
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