Sylgard 184 pdms kit

Manufactured by Dow

Sourced in United States

Sylgard 184 PDMS kit is a two-part silicone elastomer system used for various laboratory applications. It consists of a base and a curing agent that, when mixed, form a durable and flexible silicone material. The kit provides a simple and reliable method for creating custom silicone parts and coatings.

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Sylgard 184 (1702 citations) Sylgard (113 citations) PDMS Sylgard 184 (107 citations) Sylgard 184 kit (56 citations) Sylgard 184 PDMS elastomer kits (4 citations) Polydimethylsiloxane (PDMS) elastomer kits (Sylgard 184) (2 citations)

13 protocols using sylgard 184 pdms kit

PDMS Micropatterned Stamps Fabrication

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Monomer and curing agent of Sylgard 184 PDMS kit (Dow Corning) were mixed at 10:1 weight ratio. The mixture was degassed and poured on a silicon master with micro-patterns prepared by photolithography. The mixture was then cured at 37 °C for 24 hours to form a PDMS slab. The PDMS slab was peeled off from the master and cut into small stamps. The stamp had an array of circular pillars with a diameter of 5 µm and a center-to-center distance of 10 µm in the square lattice.

Song L., Yuan X., Jones Z., Vied C., Miao Y., Marzano M., Hua T., Sang Q.X., Guan J., Ma T., Zhou Y, & Li Y. (2019). Functionalization of Brain Region-specific Spheroids with Isogenic Microglia-like Cells. Scientific Reports, 9, 11055.

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Fabrication of PDMS Elastomeric Membranes

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The elastomeric membrane in each device was fabricated using a Sylgard 184 PDMS kit (Dow Corning, Midland, MI, USA). The base polymer and curing agent were mixed 10:1 by weight and allowed to degas in air for 90 min. The PDMS solution was then deposited onto a poly(methyl methacrylate (PMMA) surface and spun for 60 s at 1100–2200 RPM to achieve the desired final thickness, typically 40 or 50 μm. The coated PMMA was then baked in an oven for 1 h at 70 °C. The cured PDMS was then cut into ~5 mm squares. A Dektak Profilometer system (Bruker, Billerica, MA, USA) was used to measure the thickness of PDMS membrane—see Table 1 for measured values. A titanium flake (~100 nm thick) was transferred to the center of each PDMS square to provide a reflective surface for laser interferometric measurement of membrane displacement during testing.

Barth C, & Knospe C. (2018). Actuation of Flexible Membranes via Capillary Force: Single-Active-Surface Experiments. Micromachines, 9(11), 545.

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Superhydrophobic Silicon Surface Fabrication

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A 3″ diameter single side polished (100) plane n-type (phosphorus doped) Si wafer (Vinkarola), Sylgard 184 PDMS kit (Dow Corning), hydrofluoric acid (40 wt%), nitric acid (68 wt%), silver nitrate, n-hexane, and hydrogen peroxide (30 wt%) were purchased from Merck (Burlington, MA, USA). For coating, the structurally engineered Si wafer, 1-trichloro (1H,1H,2H,2H-perfluorooctyl) silane, was obtained from Sigma Aldrich (India). All chemicals and salts used were analytical grade. DI water (18 MΩ cm) was used to prepare all the solutions.

Bindra H.S., R. J., Kumeria T, & Nayak R. (2018). Rapid Processing of Wafer-Scale Anti-Reflecting 3D Hierarchical Structures on Silicon and Its Templation. Materials, 11(12), 2586.

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Solvent-Based Nanoparticle Dispersion Synthesis

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All materials were used as received from the suppliers unless otherwise stated. The tetrahydrofuran (THF) used for the solvent-based nanoparticle dispersion method was purchased from Sigma Aldrich (St. Louis, MO, USA). The SYLGARD 184 PDMS kit is a two-part PDMS including the base elastomer (part A) and curing agent (part B) which was purchased from Dow Corning (Freeland, MI, USA). The multi-walled CNTs were purchased from Sigma Aldrich and had a diameter between 50–80 nm and an aspect ratio >100.

Herren B., Larson P., Saha M.C, & Liu Y. (2019). Enhanced Electrical Conductivity of Carbon Nanotube-Based Elastomer Nanocomposites Prepared by Microwave Curing. Polymers, 11(7), 1212.

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Synthesis of MWCNT-PDMS Nanocomposites

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Multi-walled CNTs, with an average diameter between 50–80 nm and aspect ratio >100, were purchased from Sigma-Aldrich, USA. Tetrahydrofuran (THF) was purchased from Sigma-Aldrich, USA. The SYLGARD 184 PDMS kit was purchased from Dow Corning, USA. Cane sugar was purchased from Walmart. Unless otherwise noted, all materials were used as received and as the manufacturer recommended.

Herren B., Webster V., Davidson E., Saha M.C., Altan M.C, & Liu Y. (2021). PDMS Sponges with Embedded Carbon Nanotubes as Piezoresistive Sensors for Human Motion Detection. Nanomaterials, 11(7), 1740.

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PDMS Preparation and Slab Fabrication

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PDMS was prepared by mixing the pre-polymer and curing agent of a Dow Corning Sylgard 184 PDMS Kit in 5 : 1 ratio, curing it at RT for 24 h followed by a thermal treatment of 4 h at 80 1C under ambient conditions. Slabs of 4.5 Â 1.0 cm were cut out, cleaned with Milli-Q water and dried with nitrogen.

Glatz B.A, & Fery A. (2018). The influence of plasma treatment on the elasticity of the in situ oxidized gradient layer in PDMS: towards crack-free wrinkling. Soft matter, 15(1).

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Fabrication of PDMS Microcapsules

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Polyvinyl alcohol (PVA, 87–89% hydrolyzed, average Mw = 13,000–23,000), potassium chloride (KCl), octadecyltrichlorosilane (OTS), and methylene blue were purchased from Sigma-Aldrich (St. Louis, MO, USA). PDMS kit (Sylgard 184) and silicone oil (50 cSt, PMX-200) were purchased from Dow Corning (Midland, MI, USA). As the outer continuous phase (W_outer), aqueous solution of 5 wt% PVA was used. PVA served as a surfactant in this case. As the middle oil phase (O_middle), a mixture of PDMS and silicone oil with the volume ratio of 3:1 was employed, in which 10 wt% curing agent was added for solidifying the double-emulsion drops into microcapsules. As the inner phase (W_inner), aqueous solution of 1 wt% PVA was used. Depending on different experiments, KCl was added into the inner phase and suspending medium to adjust the osmotic pressure between the inner and outer of the PDMS shell. In some experiments, methylene blue was dissolved into the inner core as a dye for better visualization. All water used in this study was deionized (DI) water unless otherwise noted.

Guo J., Hou L., Hou J., Yu J, & Hu Q. (2020). Generation of Ultra-Thin-Shell Microcapsules Using Osmolarity-Controlled Swelling Method. Micromachines, 11(4), 444.

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Microfluidic Droplet Emulsion Preparation

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SU-8 2050 photoresist and mr-Dev 600 developer were obtained from
Microchem Corp. (Massachusetts, USA) and Micro Resist Technology (Berlin,
Germany), respectively.
1H,1H,2H,2H-Perfluorodecyltrichlorosilane
and FC-40 were purchased from ABCR-Chemicals (Karlsruhe, Germany). The PDMS kit
(Sylgard 184) was obtained from Dow Corning (Michigan, USA). The lipids
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene
glycol)-2000] (ammonium salt) (DSPE-PEG(2000) biotin) were purchased from Avanti
Polar Lipids (Alabama, USA). Alexa Fluor 488 streptavidin conjugate and DiI were
obtained from Life Technologies (Oregon, USA). Mineral oil, chloroform, dimethyl
sulfoxide (DMSO), glucose oxidase enzyme type VII (GOx), horseradish peroxidase
type VI (HRP), Amplex Red, and Amplex Ultra Red were obtained from Thermo Fisher
Scientific (Massachusetts, USA). Sodium phosphate, bovine serum albumin (BSA),
methanol, d(+)-glucose, and d(+)-sucrose were purchased from
Acros Organics (Geel, Belgium). The Alexa Fluor 488-conjugated AffiniPure goat
anti-horseradish peroxidase antibody was obtained from Jackson Immuno Research
Labs (Pennsylvania, USA). The biocompatible PFPE-PEG-PFPE triblock copolymer
surfactant introduced by Holtze et al.39 was synthesised according to a protocol by Chen
et al.40 (link)

Nuti N., Verboket P.E, & Dittrich P.S. (2017). Multivesicular droplets: a cell model system to study compartmentalised biochemical reactions. Lab on a chip, 17(18), 3112-3119.

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Fabrication of PLA-NGF Microspheres and CTAB-Coated Gold Nanorods

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For MCA synthesis, biopolymer PLA (3 mm granules, molecular weight 60,000), chloroform, and NGF-β (molecular weight 13,5 kDa) were purchased from Sigma-Aldrich (UK). The Poly(dimethylsiloxane) (PDMS) kit (Sylgard 184) was purchased from Dow-Corning (Midland, USA).
For gold nanorods (GNRs) synthesis, cetyltrimethylammonium bromide (CTAB, >98.0%), hydrochloric acid (HCl, 37 wt% in water), L-ascorbic acid (>99.9%), and sodium borohydride (NaBH₄, 99%) were purchased from Sigma-Aldrich (UK). Hydrogen tetrachloroaurate trihydrate (HAuCl₄·3H₂O) and silver nitrate (AgNO₃, >99%) were purchased from Alfa Aesar.

Sindeeva O.A., Kopach O., Kurochkin M.A., Sapelkin A., Gould D.J., Rusakov D.A, & Sukhorukov G.B. (2020). Polylactic Acid-Based Patterned Matrixes for Site-Specific Delivery of Neuropeptides On-Demand: Functional NGF Effects on Human Neuronal Cells. Frontiers in Bioengineering and Biotechnology, 8, 497.

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Fabrication of Microfluidic Emulsification Devices

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Step emulsification devices were fabricated using master molds previously utilized for the high-throughput production of particle microgels. Detailed descriptions of wafer fabrication can be found in the report by de Rutte et al.³² Devices were formed from molds using a PDMS Sylgard 184 kit (Dow Corning). The polymer base and crosslinker were combined at a 10:1 ratio, poured into the mold, degassed, and cured in a 70°C oven overnight. The following morning devices were cut from the mold and entry/exit ports were formed by biopsy punching through the polymer at either end of the device. Cut PDMS slabs were then activated alongside glass microscope slides (VWR) in an air-based plasma cleaner (Harrick Plasma) and subsequently bound together. Each formed device was incubated for another 30 min in a 70°C oven to strengthen the bond between the PDMS and glass substrate. Finally, the device was filled with Aquapel, incubated for 1 minute, and flushed with Novec-7500 fluorinated oil to render the channels hydrophobic.

Dimatteo R, & Di Carlo D. (2022). IL-2 Secretion-based Sorting of Single T Cells Using High-Throughput Microfluidic On-Cell Cytokine Capture. Lab on a chip, 22(8), 1576-1583.

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