Isopropanol

Manufactured by Tedia

Sourced in United States

Isopropanol is a clear, colorless liquid chemical compound. It has the chemical formula C₃H₇OH and is also known as isopropyl alcohol or 2-propanol. Isopropanol is a common laboratory reagent used as a solvent and disinfectant.

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Lab products found in correlation

Acetonitrile, by Tedia (4 mentions) Methanol, by Tedia (4 mentions) Cyclohexane, by Tedia (2 mentions) Ethanol, by Tedia (2 mentions) Lipid markers, by Avanti Polar Lipids (2 mentions) Hexane, by Tedia (2 mentions) Acetone, by Tedia (2 mentions) Ethyl acetate, by Tedia (2 mentions) N hexane, by Tedia (2 mentions) Ultimate 3000, by Thermo Fisher Scientific (1 mentions) Chromeleon software, by Thermo Fisher Scientific (1 mentions) Asap 2020, by Micromeritics (1 mentions) Fluoranthene, by Merck Group (1 mentions) Lpg 3400sd pump, by Thermo Fisher Scientific (1 mentions) Wps 3000 autosampler, by Thermo Fisher Scientific (1 mentions) Sep pak c18, by Waters Corporation (1 mentions) Millex hv filter, by Merck Group (1 mentions) 37 component fame mix, by Merck Group (1 mentions) Cholesta 3 5 diene, by Merck Group (1 mentions) Methanol, by Mallinckrodt (1 mentions)

15 protocols using isopropanol

Characterization of Fe3O4 Hollow Nanospheres

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Six polycyclic aromatic hydrocarbons (PAHs) standards including fluoranthene (FLU), pyrene (PYR), benz[a]anthracene (B[a]AN), benzo[a]fluorathene (B[a]FL), benzo[a]pyrene (B[a]-PY), and dibenz[a,h]anthracene (D[a,h]AN) were purchased from Sigma-Aldrich (MO, USA). Acetonitrile and isopropanol were HPLC grade and purchased from Tedia (OH, USA). Fe(NO3)3•9H2O and glycerol obtained from Shenshi Chemical Reagent and Instrumental Company (Wuhan, China) were of analytical grade. Deionized water was purified by a Milli-Q system (MA, USA).
All the experiments were carried out on Dionex UltiMate 3000 HPLC system (USA), equipped with UltiMate LPG-3400SD pump, WPS-3000 autosampler, TCC-3x00(RS) column compartment, FLD-3x00(RS) detector. Data collection was performed on Dionex Chromeleon software. The morphology and surface characteristics of the prepared Fe3O4 hollow nanospheres were characterized with a field-emission scanning electron microscope (SEM, FEI, Nova NanoSEM 450), transmission electron microscopy (TEM, TECNAI G2 20 U-Twin instrument, Netherlands) and surface area and pore size analyzer (Micromeritics ASAP 2020, USA).

Xi J., Zhang J, & Zhao H. (2017). Novel Uniform Fe₃O₄ Hollow Spheres for Magnetic Solid-phase Extraction of Polycyclic Aromatic Hydrocarbons. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 33(9).

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Analytical Standards for Lipid Analysis

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The following analytical standards were acquired from Cambridge Isotope Laboratories (5MChr), ChemService (DaiP), Fluka (BjF, DalP, DaeP, DahP), Supelco Inc. (BaA, Chr, BbF, BkF, BaP, DahA, IcdP and 37 component FAME mix) and Sigma (5α-cholestan, cholesta-3,5-diene, campesterol, stigmasterol, β-sitosterol, brassicasterol). High-performance liquid chromatography (HPLC)-grade hexane, cyclohexane, isopropanol and N,N-dimetilformamide were from Tedia, acetonitrile from J.T. Baker, methanol from Mallinckrodt and water was obtained from a Millipore Milli-Q purification system. Millex HV filters (0.45 µm) were purchased from Millipore and solid-phase extraction (SPE) columns were from Waters (Sep Pak C18, 500 mg, 3 mL).

Tfouni S.A.V., Reis R.M., Amaro N.d.P.L., Pascoal C.R., de Camargo M.C.R., Baggio S.R., Rauen‐Miguel A.M, & Furlani R.P.Z. (2017). Adulteration and Presence of Polycyclic Aromatic Hydrocarbons in Extra Virgin Olive Oil Sold on the Brazilian Market. Journal of the American Oil Chemists' Society, 94(11).

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Phytochemical Isolation and Bioassay

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4,8-DHT was isolated and purified from C. callicarpa epicarp in our laboratory (purity ≥ 98%). 1,3,5-tri-tert-butylbenzene was purchased from Sigma Aldrich (St. Louis, MO, USA). Seeds of lettuce (Latuca sativa), radish (Raphanus sativus), cucumber (Cucumis sativus), onion (Allium cepa), and wheat (Triticum aestivum) were purchased from the market in Ningbo, China, and used for bioassay. HPLC grade of iso-butanol, n-butanol, iso-propanol, n-propanol, and ethanol were purchased from Tedia Company Inc. (Fairfield, IA, USA). All other chemicals and solvents in analytical grade were purchased from commercial sources.

Yang L., Ma X.Y., Ruan X., Jiang D.A., Pan C.D, & Wang Q. (2016). Enantioselective Separation of 4,8-DHT and Phytotoxicity of the Enantiomers on Various Plant Species. Molecules, 21(4), 528.

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Synthesis of Iron-doxorubicin Nanoparticles

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Agar was purchased from Fisher chemical, Pittsburgh, PA, USA. Iron (II) chloride and iron (III) chloride were from Merck, Germany. DOX was from Sigma-Aldrich, Saint Louis, MO, USA. MTT test was from EMD Millipore, USA. Isopropanol was from TEDIA, USA. Phosphate-buffered saline (PBS) solution (pH 7) was from UniRegion Bio-Tech, Taiwan and used as obtained without further purification. Milli-Q reagent-grade water (18.2 MΩ cm at 25 °C) was used for all synthesis processing steps that required water.

Wang Y.J., Lin P.Y., Hsieh S.L., Kirankumar R., Lin H.Y., Li J.H., Chen Y.T., Wu H.M, & Hsieh S. (2021). Utilizing Edible Agar as a Carrier for Dual Functional Doxorubicin-Fe3O4 Nanotherapy Drugs. Materials, 14(8), 1824.

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Enzymatic Transesterification of Flavonoid Rutin

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The analytical reactions were conducted with the commercial flavonoid rutin (Sigma-Aldrich) as substrate. First, 45 mg of the flavonoid, 3.0 mL of vinyl acetate (acyl donor) (Spectrum^®) and 1 mL of isopropanol (Tedia) were mixed, and after 5 min 18 mg of the commercial enzyme Novozym 435 (947.20 U/g) (Candida antarctica) was added. The reactions were conducted on an orbital shaker (Solab SL-223) at 60 °C, with stirring at 200 rpm. Aliquots (500 μL) were withdrawn from 24 to 120 h, and then analyzed by different chromatographic techniques. After the optimum conditions were established, the reactions were conducted on a larger scale in order to obtain the products in larger amounts and to perform a purification step. The scaled-up transesterification reaction was performed using the same proportions of reagents and biocatalyst: 300 mg of rutin, 20 mL of vinyl acetate, 6.7 mL of isopropanol, and 120 mg of Novozym 435 [7 (link)].

Mecenas A.S., Adão Malafaia C.R., Sangenito L.S., Simas D.L., Machado T.D., Amaral A.C., dos Santos A.L., Freire D.M, & Leal I.C. (2018). Rutin derivatives obtained by transesterification reactions catalyzed by Novozym 435: Antioxidant properties and absence of toxicity in mammalian cells. PLoS ONE, 13(9), e0203159.

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Nucleic Acid Extraction and Quantification

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Unlabeled SEP was provided by the School of Life Science and Technology, China Pharmaceutical University (Nanjing, China). Scintillation fluid was from PerkinElmer Life Science (Boston, MA). Isopropanol was purchased from Tedia (USA). Purified water was prepared using the Milli-Q water purification system (Millipore, USA). Other chemicals used in the experiment were commercially available analytical reagents.

Xing H., Zhu X., Liao J., Kong Y., Lu Y., Zhao D., Li N., Chen X, & Qin Z. (2023). Pharmacokinetic study of Strongylocentrotus nudus egg polysaccharide in rats and beagles using a 3H-labeling method. Frontiers in Pharmacology, 14, 1109084.

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Lipid Marker Characterization Protocol

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Lipid markers were obtained from Avanti Polar Lipids (Alabaster, AL). All the authentic standards were purchased from Sigma-Aldrich (St. Louis, MO, US). Methanol, isopropanol and acetonitrile were purchased from TEDIA (Fairfield, OH, USA). Dextran sulfate sodium (DSS) was purchased from MP Biomedicals (Solon, OH, USA) (M_W = 36000–50000).

Wang R., Gu X., Dai W., Ye J., Lu F., Chai Y., Fan G., Gonzalez F.J., Duan G, & Qi Y. (2016). A lipidomics investigation into the intervention of celastrol in experimental colitis. Molecular bioSystems, 12(5), 1436-1444.

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Total RNA Extraction and RT-qPCR Analysis

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Total cellular RNA was extracted using TRIzol (Sigma-Aldrich). The cells were pipetted with TRIzol (40 μl/10⁵ cells) until completely lysed, and chloroform (Tedia) was added for stratification by the gradient. After centrifugation (4°C, 12,000 g/min, 15 min), the transparent supernatant was collected. Isopropanol (Tedia) was used to precipitate RNA, and 75% ethanol was for purification. The remaining RNA pellet was resuspended in enzyme-free water, followed by incubation at 55°C for 15 min. The quantification was performed using NanoDrop 2000C (United States). The cDNA was synthesized with PrimeScript RT Master Mix (Takara) using 1 μg RNA. cDNA samples were used for RT-qPCR with SYBR Premix Ex Taq^TM (Takara) according to the operation manual. The sequences of primer pairs are listed in Table S2. Relative mRNA expressions were analyzed based on the 2^−ΔΔCt method, normalizing to the housekeeping gene GAPDH.

Table S2. Primer list.

Zhang J., Yuan Z., Li X., Wang F., Wei X., Kang Y., Mo C., Jiang J., Liang H, & Ye L. (2023). Activation of the JNK/COX-2/HIF-1α axis promotes M1 macrophage via glycolytic shift in HIV-1 infection. Life Science Alliance, 6(12), e202302148.

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Carotenoid and Vitamin E Extraction

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For the extraction of carotenoids and vitamin E, the following analytical-grade reagents were used: acetone and petroleum ether (Vetec, Brazil). The following HPLC-grade reagents were used for the analysis: acetone, hexane, isopropanol, ethyl acetate, methanol and acetonitrile (Tedia, Brazil), and glacial acetic acid (Vetec, Brazil).
The vitamin E standards (α-, β-, γ-, and δ-tocopherols and tocotrienols) were acquired from Calbiochem®, EMD Biosciences, Inc. (USA). The α-carotene and β-carotene patterns were isolated from concentrated carrot extract; β-cryptoxanthin and lycopene were isolated from tomato and papaya extracts, respectively, by open-column chromatography [25 (link)].
For the filtration of the samples, it was used filter paper no. JP41 J. (Prolab, Brazil), HV Millex filter units, in polyethylene with 0.45 μm of porosity (Millipore, Brazil) and 3 ml sterilized disposable syringes (TKL, China).

de Paula Filho G.X., Barreira T.F, & Pinheiro-Sant'Ana H.M. (2022). Chemical Composition and Nutritional Value of Three Sonchus Species. International Journal of Food Science, 2022, 4181656.

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Lipid Marker Acquisition Protocol

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Lipid markers were obtained from Avanti Polar Lipids (Alabaster, AL, USA). All authentic standards were purchased from SigmaeAldrich (St. Louis, MO, USA). Methanol, isopropanol, and acetonitrile were purchased from TEDIA (Fairfield, OH, USA).

Zhou X., Wang R., Zhang T., Liu F., Zhang W., Wang G., Gu G., Han Q., Xu D., Yao C., Guo D., Fu W., Qi Y, & Wang L. (2019). Identification of Lysophosphatidylcholines and Sphingolipids as Potential Biomarkers for Acute Aortic Dissection via Serum Metabolomics. European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, 57(3).

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