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Elisa maxtm standard

Manufactured by BioLegend

ELISA MAXTM Standard is a lab equipment product designed for enzyme-linked immunosorbent assay (ELISA) applications. It serves as a standard for quantifying target analytes in ELISA experiments.

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3 protocols using elisa maxtm standard

1

C. difficile Ribotypes Induce IL-12 and IL-18 in PBMCs

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0.5 × 106 PBMCs were either left untreated or stimulated with C. difficile ribotypes RT084, RT012, RT001, RT023, and RT027 at MOI 1 for 20 h at 37°C in duplicates. Supernatants of respective samples were pooled and enzyme-linked immunosorbent assays were performed to detect human IL-12 using Human IL-12 (p70) ELISA MAXTM Standard (BioLegend) and human IL-18 using IL-18 Human ELISA Kit (Invitrogen).
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2

Cytokine Secretion Assay in BMDM

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CCL2 and IL6 ELISA was performed on media from BMDM cells after 6 h induction with ethanol, 1 μM DEX or 10 μM CpdA in combination with 100 ng/ml LPS and 10 μM chloroquine (added 30 min prior to the induction with compounds) by using the ELISA MAXTM Standard (BioLegend, 432,702, 430,502), according to the manual.
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3

Cytokine Release Analysis Protocol

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The in vivo and in vitro samples were analyzed for cytokine release by using ELISA MAXTM Standard (mouse IL-1β, 432601; mouse IL-6, 431301; mouse IL-1α, 433401; BioLegend) as per the manufacturer’s instructions.
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