Neurons were randomly divided into five groups: a control group, a sham group, and three groups which received magnetic stimulation at 40% (1 Hz, 1.68 T), 60% (1 Hz, 2.52 T), and 100% (1 Hz, 4.2 T).8 (link) The control group was placed in the incubator without any treatment. For the stimulated groups, rTMS was delivered with a MCF-B65 butterfly coil9 (link) (Medtronic company, Denmark) with a 80 mm outer diameter connected to a MagPro R30 magnetic stimulation device of 4.2 T maximum output. The coil produced a focal field with the maximal intensity at the intersection of the round components.10 (link) The stimulator handle was fixed on the top of each set of six-well plates with neuron culture platforms by a bracket and parallelled to the Petri dish at a distance of about 1 cm,9 (link) without direct contact.11 (link) Stimulation occurred at a fixed time every day for 5 consecutive days, with the stimulus parameters set at 1 Hz frequency, and intensity of 1.68, 2.52, and 4.2 T corresponding to the 40%T, 60%T and 100%T group, respectively. The stimulus pattern consisted of 3 pulse trains, each containing 100 pulses at 1 Hz, and 60-s interval among sequences. Cells in the sham stimulation group were placed in the magnetic field environment with the same coil, yet the coil was disconnected from the power supply.
Magpro r30
Manufactured by Medtronic
Sourced in Denmark
The MagPro R30 is a magnetic stimulator designed for neurophysiological and diagnostic applications. It is capable of generating high-intensity magnetic pulses to non-invasively stimulate the nervous system.
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Lab products found in correlation
3 protocols using magpro r30
1
Transcranial Magnetic Stimulation on Neurons
2
Transcranial Magnetic Stimulation for Corticospinal Excitability
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Transcranial magnetic stimulation (MagPro R30, Medtronic) was delivered over the left motor cortex to elicit motor evoked potentials (MEPs) in the FCR and BB muscle to test corticospinal excitability. The location for stimulation was chosen by first determining the optimal site for FCR MEPs by periodically moving the coil to identify the location that produced the largest MEP. This location was then marked and maintained within 1 mm relative to cortical landmark throughout the experiment using an image guidance system (Brainsight, Rogue Research) to ensure accurate and consistent stimulation. Stimulation intensity was set at the beginning of the experimental protocol and maintained across ULV and CONTROL conditions to evoke an MEP that was ≈70% of the maximal MEP amplitude so that both inhibition and facilitation of the MEP could occur.
3
Supplementary Motor Area rTMS for Neurological Treatment
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Active group participants received low-frequency active rTMS (1 Hz, 110% of the Resting Motor Threshold (RMT), for 20 min (5 min trains with 2 min intertrain intervals) by figure eight shaped coils; Medtronic MagPro R30, Farum, Denmark) bilaterally to Supplementary Motor Area (SMA) five sessions a week for the first four weeks. During the fifth week, sessions were reduced to three times per week and again to twice a week during the sixth week. Sham group patients received a coil that was held 90 degrees from the skull, with an intensity of 110% of RMT. The remaining procedure was the same as the active group. Participants in the Istanbul, Turkey site did not participate in the six-week follow-up after the end of treatment.
The stimulus site was 15% of the distance between inion and nasion, anterior to vertex (Cz), which corresponds to the bilateral SMA according to the 10–20 International EEG localization system. The RMT was determined using single-pulse TMS, given stimuli localizing the coil to the left side of the skull matching to the right hand area according to 10–20 EEG localization system.
The stimulus site was 15% of the distance between inion and nasion, anterior to vertex (Cz), which corresponds to the bilateral SMA according to the 10–20 International EEG localization system. The RMT was determined using single-pulse TMS, given stimuli localizing the coil to the left side of the skull matching to the right hand area according to 10–20 EEG localization system.
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