B was purchased from Baoji Guokang Bio-Technology Co.,Ltd (China). Chitosan (20 KDa), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) and acetic acid were obtained from Merck company (Germany). Also, culture medium RPMI-1640, Fetal Bovine Serum (FBS) and Penicillin/Streptomycin antibiotics were supplied by Gibco Company (USA). Furthermore, Tripolyphosphate (TPP), DMSO and Schneider culture medium were prepared form Sigma-Aldrich (USA). Iranian strain of L. major (MRHO/IR/75/ER) was supplied by Pasteur Institute of Iran. All other materials were of analytical grade. Double distilled water was used throughout the study.
Schneider culture medium
Manufactured by Merck Group
Sourced in United States
Schneider culture medium is a nutritional solution used to cultivate a variety of microorganisms, including bacteria, fungi, and algae. It provides the necessary nutrients and growth factors to support the proliferation of these microorganisms in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Merck Group (2 mentions)
Streptomycin, by Merck Group (2 mentions)
Acetic acid, by Merck Group (1 mentions)
Foetal bovine serum (fbs), by Merck Group (1 mentions)
Schneider medium, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Modified eagle s medium mem, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
6 protocols using schneider culture medium
1
Chitosan-TPP Nanoparticles for Leishmania Treatment
2
Cultivation of Leishmania braziliensis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The strain of L. (V.) braziliensis (MHOM-BR-94-H3227) was grown in Schneider culture medium (Sigma Chem. Co., St. Louis, Mo, USA), containing 10% heat-inactivated foetal bovine serum (Sigma), 200 U penicillin/mL, and 200 mg streptomycin/μL (Sigma). The strain of L. braziliensis (MHOM-BR-94-H3227) was isolated from a patient with cutaneous leishmaniasis and characterized by polymerase chain technique and by monoclonal antibodies [28 (link)].
3
Culturing and Preserving Leishmania amazonensis Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The amastigote cultures of L. amazonesis MHOM/BR/pH8 strain were kept under cryopreservation until transferred to NNN medium (Novy–MacNeal–Nicolle) and cultured at 26 °C until the parasites reached the log growth phase. Then, the suspension was transferred to Schneider culture medium (Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% inactivated fetal bovine serum and 0.2% gentamicin sulfate, at 26 °C, so that the parasites returned to the log phase of growth. After that stage, the promastigote forms were incubated at 37 °C, producing a growth curve and the formation of the axenic amastigote strains [79 (link)].
The promastigote forms of L. amazonensis MHOM/BR/pH8 strain were preserved in Schneider medium (Sigma-Aldrich, St Louis, MO, USA) supplemented with 20% fetal bovine serum (FBS) and 1% streptomycin/penicillin, at 26 °C, in a B.O.D incubator—J. Prolab, São José dos Pinhais, Brasil, model JP. 100 (LBCM/CPAM). The promastigote forms were used in the exponential growth phase in all stages of the experiment [80 (link)].
The promastigote forms of L. amazonensis MHOM/BR/pH8 strain were preserved in Schneider medium (Sigma-Aldrich, St Louis, MO, USA) supplemented with 20% fetal bovine serum (FBS) and 1% streptomycin/penicillin, at 26 °C, in a B.O.D incubator—J. Prolab, São José dos Pinhais, Brasil, model JP. 100 (LBCM/CPAM). The promastigote forms were used in the exponential growth phase in all stages of the experiment [80 (link)].
4
Cultivation of Leishmania amazonensis Promastigotes
Check if the same lab product or an alternative is used in the 5 most similar protocols
Leishmania amazonensis MHOM/Br/00/BA125 strain was used for DCs infection. L. amazonensis promastigotes were maintained in Schneider culture medium (sigma®) supplemented with 10% fetal calf serum (sigma®), 2 mm L-glutamine, 100 μg/ml penicillin and 100 μg/ml streptomycin (sigma®) in an incubator at 24°c. During the culture period, promastigotes were counted in the Neubauer chamber with a 100x dilution in saline in order to measure parasite growth dynamics. In all experiments, stationary phase parasites were used which are enriched by infective stage of parasite, called metacyclic promastigotes.
+ Expand
Leishmania amazonensis MHOM/Br/00/BA125 strain was used for DCs infection. L. amazonensis promastigotes were maintained in Schneider culture medium (sigma®) supplemented with 10% fetal calf serum (sigma®), 2 mm L-glutamine, 100 μg/ml penicillin and 100 μg/ml streptomycin (sigma®) in an incubator at 24°c. During the culture period, promastigotes were counted in the Neubauer chamber with a 100x dilution in saline in order to measure parasite growth dynamics. In all experiments, stationary phase parasites were used which are enriched by infective stage of parasite, called metacyclic promastigotes.
5
Chitosan-TPP Nanoparticles for Leishmania Inhibition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Amp was supplied by Biobasic (Canada). Also, chitosan (20 KDa), tripolyphosphate (TPP), acetic acid, dimethylsulfoxide (DMSO), schneider culture medium, and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) were purchased from Sigma-Aldrich (USA). RPMI-1640, Fetal Bovine Serum (FBS) and Penicillin/Streptomycin antibiotics were obtained from Gibco Company (USA). Furthermore, the L. major strain of MRHO/IR/75/ER (an Iranian strain) was obtained from Pasteur Institute of Iran. All other chemicals were of analytical grade.
6
Lipid-based Formulations of Amphotericin B
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Amphotericin B and AmBisome were gift from GeMePe, Argentina. Soybean phosphatidylcholine (SPC) (phospholipon 90 G, purity >90%) was a gift from Phospholipid/Natterman, Germany. Sodium cholate (NaChol), Tween 80 (T80), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), 3-(N-morpholino) propansulfonic acid (MOPS), RPMI-1640 medium and Schneider culture medium were from Sigma-Aldrich, Argentina. Disodium succinate was from Baker Analyzed, USA. Modified Eagle's medium (MEM) was from Gibco, Argentina. Fetal calf serum (FCS), antibiotic/antimycotic solution (penicillin 10000 UI/ml, streptomycin sulphate 10 mg/ml, amphotericin B 25 g/ml), glutamine and trypsin/EDTA were from PAA Laboratories GmbH, Austria. Methanol, chloroform, hydrochloric acid and perchloric acid were from Anedra, Argentina.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!