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Ef 100mm f 2.8l macro is usm lens

Manufactured by Canon

The Canon EF 100mm f/2.8L Macro IS USM lens is a high-performance macro lens designed for Canon EOS DSLR cameras. It features a maximum aperture of f/2.8 and a focal length of 100mm, allowing for close-up photography with a 1:1 life-size magnification ratio. The lens is equipped with an Optical Image Stabilizer system, providing up to 4 stops of shake correction, and utilizes a ring-type Ultrasonic Motor for quiet and precise autofocus operation.

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9 protocols using ef 100mm f 2.8l macro is usm lens

1

Cryomacrotome Sectioning and Digital Imaging

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The frozen cadaveric head was sectioned serially using a cryomacrotome. Sectioning was performed serially at 0.04-mm intervals. After every sectioning, frost and foreign substances on the sectioned surface were wiped away with 99% ethyl alcohol. Protruding objects on the surface were removed using a scalpel (Fig. 1).
A Canon™ EOS 5DsR digital single lens reflex camera and Canon™ EF 100mm f/2.8L Macro IS USM Lens were employed (resolution, 8,688 × 5,792 pixels). The distance from the digital camera to the sectioned surface was adjusted to yield a photograph area of 347.5 mm in horizontal length and 231.7 mm in vertical length on the sectioned surface, corresponding to a pixel size of 0.04 mm × 0.04 mm. Two Elinchrom™ Digital S Strobes with an Elinchrom™ Digital 2 power pack were used to maintain a constant brightness of the sectioned surface. A Tiffen™ Color Control Patch was placed on the sectioned surface and photographed every day for post processing of these images. The sectioned surface was photographed using a digital camera (ISO 100, shutter speed 1/250, aperture F/13, manual focus). The photograph was assessed by anatomists on a computer monitor using Adobe Photoshop CS6 (Adobe Systems, Inc., San Jose, CA, USA).
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2

Anatomy and Genitalia of Southeastern China Moths

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The examined specimens were collected from Guangxi and Fujian Provinces in southeastern China in 2018. The descriptive terminology of the anatomical structures generally follows Wang (2006a) , however in descriptions of the male genitalia, the more proper term phallus rather than aedeagus is applied here following Kristensen (2003) . Photographs of adults were taken using a Canon EOS 6D Mark II camera plus an EF 100 mm f/2.8L MACRO IS USM lens with the help of EOS Utility 3.10.20 software. Images of genitalia were captured using a Leica DM4 B upright microscope and photomontage was performed with Leica Application Suite X imaging software. All type specimens are deposited in the Morphological Laboratory, Guizhou University of traditional Chinese Medicine, Guiyang 550025, Guizhou, China.
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3

Stereomicroscopy of Preserved Insect Specimens

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Images of individuals were taken as multi-layer montages using a Leica M205C stereomicroscope with a Leica DFC 425 module run with Leica Application Suite software version 3. Preserved specimens, stored in 85% ethanol, were positioned in a transparent petri dish filled with Purell hand sanitizer (70% EtOH). Measurements (Tables 12) were obtained using an Olympus SZH stereomicroscope fitted with an ocular micrometer. A field photograph of live specimens placed in a small paper-lined Petri dish was taken with a Canon EOS 5DS R combined with a Canon EF 100mm f/2.8L Macro IS USM lens. Morphological terminology follows that of Krishna (1961) .
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4

Automated Time-Lapse Imaging of Montipora capricornis

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We used a Canon EOS 5D Mark IV camera equipped with an EF 100mm f/2.8L Macro IS USM lens and the timer option to automatically take pictures of the Montipora capricornis every 2 minutes over a period of 105 days. The frequency of taking images is determined by the frequency of tissues motions of Montipora capricornis [21 (link)]. The parameters for the camera were: F11, ISO4000, 1/10s. The camera was mounted to a tripod for stability over the long data collection period. The macro lens was perpendicular to the wall of the aquarium to avoid the blurred effect caused by refraction. Besides, the aquarium wall was cleaned regularly to ensure the quality of images. The distance between the macro lens and the wall of the aquarium was 12.5 cm.
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5

Collecting and Preserving A. aurantii Aphids

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A total of 48 A. aurantii specimens were collected from host plants of 11 families. The live morphology and habitats of A. aurantii in the field were photographed with digital cameras (Cannon EOS 7D plus Canon EF 100 mm f/2.8LMacro IS USM Lens). After recording the ecological information, aphid clones were stored in 95% ethanol and kept at -20 • C for further morphological measurement and molecular experiments. All samples and voucher specimens were deposited in the Insect Systematics and Diversity Lab at Fujian Agriculture and Forestry University. Detailed information (host plant, voucher number, and GenBank accession number) of the specimens were listed in Supplementary Table 1.
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6

Scorpion Habitat Selection and Identification

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A pilot study was carried for two days in early July for habitat selection and species identification before the survey. All possible microhabitats, including both terrestrial and arboreal, were thoroughly observed using the direct visual encounter method with the aid of UV lights. Sampling was carried out by two to three observers and lasted for about seven hours (19.00-02.00 h). A total of 78 human hours were spent equally for open and forest habitats (39 human hours per each habitat). Abundance and age-sex classes were recorded as male, female, or juvenile. But burrowing scorpions were not classified into age/sex categories due to difficulties in excavating their burrows and habitat disruptions. Tree barks were observed up to 3m in height from the ground level. Tree heights were categorized into five height classes as 1: 0-60 cm, 2: 61-120 cm, 3: 121-180 cm, 4: 181-240 cm and 5: 241-300 cm. Tree diameter at breast height (DBH) was measured using a DBH tape. Tree DBH measures were categorized into five classes as 1: 0-120 cm, 2: 121-240 cm, 3: 241-360 cm, 4: 361-480 cm and 5: 481-600 cm. Photographs were taken using a Canon 750D camera with Canon EF 100mm f/2.8L Macro IS USM lens with an external flashlight. Identifications of the species were based on Kovařík et al. (2016) .
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7

High-Resolution Fingerprint Imaging Protocol

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The images used in this report were taken using a digital single lens reflex (DSLR) camera mounted on a Kaiser RS1 copy stand. The DSLR was a Canon EOS 700D which was fitted with a Canon Angle Finder C 90° viewfinder with a 1.25-2.5x optical magnification and a Canon TC-80N3 remote control external shutter release to avoid camera motion. Images were taken using two sizes of macro lenses-a 50mm lens for overview shots of the substrates and a 100mm lens for high magnification macro shots of individual fingerprints. The 50mm lens was used as it is recognised as being generally equivalent to the view seen by the human eye [10] . The lenses used were a Canon EF 50mm f2.5 macro lens and a Canon EF 100mm f2.8L macro IS USM lens. Substrates were lit using oblique lighting from two Daylight Twist Portable Lamps with a white light output of 6500K. Images were captured in aperture priority mode with an aperture of f/8 to ensure clarity of focus across the whole fingerprint and an ISO between 100 and 400 to minimise noise or grain in the images. The lighting setup in combination with the selected DSLR camera settings were determined in preliminary studies where clear second level ridge detail could be observed. DSLR images were saved onto a SanDisk 16GB memory card and transferred to a PC for grading.
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8

Fingerprint Imaging Protocol for Forensic Analysis

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The images used in this report were taken using a digital single lens reflex (DSLR) camera mounted on a Kaiser RS1 copy stand. The DSLR was a Canon EOS 700D which was fitted with a Canon Angle Finder C 90° viewfinder with a 1.25-2.5x optical magnification and a Canon TC-80N3 remote control external shutter release to avoid camera motion. Images were taken using two sizes of macro lenses-a 50mm lens for overview shots of the substrates and a 100mm lens for high magnification macro shots of individual fingerprints. The lenses used were a Canon EF 50mm f2.5 macro lens and a Canon EF 100mm f2.8L macro IS USM lens. Substrates were lit using oblique lighting from two Daylight Twist Portable Lamps with a white light output of 6500K. Images were captured in aperture priority mode with an aperture of f/8 to ensure clarity of focus across the whole fingerprint and an ISO between 100 and 400 to minimise noise or grain in the images. The lighting setup in combination with the selected DSLR camera settings were determined in preliminary studies where clear second level ridge detail could be observed. DSLR images were saved onto a SanDisk 16GB memory card and transferred to a PC for grading.
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9

Photographic Techniques for Biological Imaging

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Photographs of live animals displayed in Figure 2 were taken with a Canon T2i DSLR and a Canon EF 100mm f/2.8L Macro IS USM lens (Canon, Melville, NY). Raw photos were post processed for brightness, contrast, and color balance using Adobe Lightroom 5 photo editing software (Adobe Systems, San Jose, CA). Light photomicroscopy on fixed material was carried out using a Nikon (SMZ25; Minato, Tokyo, Japan) stereomicroscope with the SHR Plan Apo 2Â objective. Images were captured using NIS-Elements software (Nikon). Fresh material was usually photographed on compound microscopes using 20Â objectives with illumination from above both sides of the samples at roughly 457. Images were captured using a Canon T2i DSLR with a microscope ocular adaptor lens. Focal series were captured through the depth of the radiole, and they assembled into a focus-stacked image using Zerene Stacker software (Zerene Systems, Richland, WA).
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