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Conta resin

Manufactured by Takara Bio
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CoNTA resin is a nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography resin used for the purification of recombinant proteins containing a histidine-tag. The resin binds to the histidine-tag with high affinity, allowing the target protein to be selectively captured and purified from complex mixtures.

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Lab products found in correlation

Exogenous furin, by New England Biolabs (2 mentions) Expi293f cells, by Thermo Fisher Scientific (2 mentions) Strep tactin xt resin, by IBA Lifesciences (2 mentions)

2 protocols using conta resin

1

Recombinant ACE2 and SARS-CoV-2 Spike Protein Production

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The ectodomains of ACE2 (19–615) and stabilized, ‘2P’ mutant (K986P and V987P) of SARS-CoV-2 spike (residues 1–1208) with intact furin-cleavage site were prepared as described in a recent study26 (link). In brief, the proteins were expressed in Expi293F cells (Gibco), collected twice after 3–4 and 6–7 days, and purified with affinity chromatography (spike using CoNTA resin from TAKARA, ACE2 with Streptactin XT resin from IBA Lifesciences), followed by gel filtration into a buffer containing 20 mM Tris pH 8.0 and 150 mM NaCl. As previously described26 (link), the purified spike was then incubated for 5 h with exogenous furin (New England Biolabs), after which the reaction was stopped by addition of EDTA.
Benton D.J., Wrobel A.G., Xu P., Roustan C., Martin S.R., Rosenthal P.B., Skehel J.J, & Gamblin S.J. (2020). Receptor binding and priming of the spike protein of SARS-CoV-2 for membrane fusion. Nature, 588(7837), 327-330.
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2

Recombinant ACE2 and SARS-CoV-2 Spike Protein Production

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The ectodomains of ACE2 (19–615) and stabilized, ‘2P’ mutant (K986P and V987P) of SARS-CoV-2 spike (residues 1–1208) with intact furin-cleavage site were prepared as described in a recent study26 (link). In brief, the proteins were expressed in Expi293F cells (Gibco), collected twice after 3–4 and 6–7 days, and purified with affinity chromatography (spike using CoNTA resin from TAKARA, ACE2 with Streptactin XT resin from IBA Lifesciences), followed by gel filtration into a buffer containing 20 mM Tris pH 8.0 and 150 mM NaCl. As previously described26 (link), the purified spike was then incubated for 5 h with exogenous furin (New England Biolabs), after which the reaction was stopped by addition of EDTA.
Benton D.J., Wrobel A.G., Xu P., Roustan C., Martin S.R., Rosenthal P.B., Skehel J.J, & Gamblin S.J. (2020). Receptor binding and priming of the spike protein of SARS-CoV-2 for membrane fusion. Nature, 588(7837), 327-330.
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