Log In Sign up
The largest database of trusted experimental protocols

Fatty acid synthase

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

Fatty Acid Synthase is a multi-enzyme complex that catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. It is an essential enzyme involved in the de novo synthesis of fatty acids.

Automatically generated - may contain errors

Lab products found in correlation

Acetyl coa carboxylase, by Cell Signaling Technology (6 mentions) Pparγ, by Cell Signaling Technology (3 mentions) C ebpα, by Cell Signaling Technology (3 mentions) Stearoyl coa desaturase 1, by Cell Signaling Technology (2 mentions) Insulin, by Merck Group (2 mentions) Dexamethasone (dex), by Merck Group (2 mentions) Phospho acetyl coa carboxylase, by Cell Signaling Technology (2 mentions) Catalase, by Cell Signaling Technology (2 mentions) Irdye 800cw goat anti rabbit, by LI COR (2 mentions) Atp citrate lyase, by Proteintech (2 mentions) Acyl coa synthetase family member 2, by Cell Signaling Technology (2 mentions) S6 ribosomal protein, by Cell Signaling Technology (2 mentions) Odyssey clx, by LI COR (2 mentions) Tissuelyser, by Qiagen (2 mentions) Phospho acc ser79, by Cell Signaling Technology (2 mentions) β actin, by Merck Group (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) α tubulin, by Merck Group (2 mentions) Ripa buffer, by Thermo Fisher Scientific (2 mentions) Bromodeoxyuridine (brdu), by Cell Signaling Technology (1 mentions)

19 protocols using fatty acid synthase

1

Comprehensive Protein Extraction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total proteins were extracted through 30-minute incubation on ice with Nonidet P-40 lysis buffer containing protease and phosphatase inhibitors, and resolved on Bolt Bis-Tris Plus polyacrylamide gels (Life Technologies). Antibodies against PARP (cat# 9532S), Phospho-Akt (S473; cat# 4060S), Akt (cat# 4685S), Phospho-Erk (T202/Y204; cat# 9101S), Erk1/2 (cat# 9102S), Phospho-S6 ribosomal protein (S235/236; cat# 2211S), total S6 ribosomal protein (cat# 2317S), Phospho-S6 kinase (cat# 9234S), total S6-kinase (cat# 2708S), Tuberin/TSC2 (cat# 4308S), Phospho-RSK (S380) (cat# 9335), total RSK (cat# 9355), Fatty Acid Synthase (cat# 3180S), Acetyl-CoA Carboxylase (cat# 3676S), Stearoyl-CoA desaturase 1 (cat# 2794S), CCTα (cat# 6931S) and BrdU (5292S) were obtained from Cell Signaling Technology (Danvers, MA). Anti-beta actin antibody (cat# A5316) was obtained from Millipore Sigma (St. Louis, MO) and anti-CPT1A antibody (cat# ab128568) from Abcam (Cambridge, MA).
Feng Y., Mischler W.J., Gurung A.C., Kavanagh T.R., Androsov G., Sadow P.M., Herbert Z.T, & Priolo C. (2020). Therapeutic targeting of the secreted lysophospholipase D autotaxin suppresses tuberous sclerosis complex-associated tumorigenesis. Cancer research, 80(13), 2751-2763.
+ Open protocol
+ Expand
2

3T3-L1 Adipocyte Differentiation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
3T3-L1 preadipocytes were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Dulbecco’s modified Eagle’s medium (DMEM) and penicillin-streptomycin were purchased from Mediatech (Herndon, VA, USA). Oil red O solution, isobutylethylxanthine (IBMX), dexamethasone, and insulin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Primary antibodies including phospho (P)-Akt1 (T308), total (T)-Akt1, P-ERK1/2 (T202/Y204), T-ERK1/2, C/EBPα, PPARγ, Fatty acid synthase (FAS), Acetyl-CoA carboxylase (ACC) and GAPDH were purchased from Cell Signaling Technology (Boston, MA, USA).
Chayaratanasin P., Caobi A., Suparpprom C., Saenset S., Pasukamonset P., Suanpairintr N., Barbieri M.A, & Adisakwattana S. (2019). Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression. Molecules, 24(10), 1894.
+ Open protocol
+ Expand
3

Molecular Mechanisms of EMT and Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human TNFα and TGFβ were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies used are listed as follows: IkB, phospho-Smad2/3, total-Smad2/3, Snail, vimentin, lactate dehydrogenase-A, fatty acid synthase, stearoyl-CoA desaturase-1, phospho-acetyl-CoA carboxylase, total-acetyl-CoA carboxylase (Cell signaling, MA, USA); beta-actin, E-cadherin, N-cadherin (Santa Cruz, TX, USA); hexokinase II, pyruvate kinase isoform 2, fructose-bisphophotase-2, total OXPHOS Human WB Antibody Cocktail (Abcam, Cambridge, UK); Zeb1 (ProSci, CA, USA); and fructose-bisphophotase-1 (Abgent, Wuxi, China). All other reagents were from Sigma-Aldrich (MO, USA) unless stated otherwise.
Liu M., Quek L.E., Sultani G, & Turner N. (2016). Epithelial-mesenchymal transition induction is associated with augmented glucose uptake and lactate production in pancreatic ductal adenocarcinoma. Cancer & Metabolism, 4, 19.
+ Open protocol
+ Expand
4

Immunohistochemical Analysis of Molecular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunohistochemistry, tissue sections were deparaffinized, and blocked with 10% or 100% goat serum at room temperature for 2 hr. Tissue sections were incubated at overnight at 4°C with rabbit affinity purified polyclonal antibodies against cleaved caspase-3 (1:200, Cell Signaling, Danvers, MA), fatty acid synthase (1:100, Cell Signaling), fibroblast growth factor receptor 2 (FGFR2) (1:2000, Abcam, Cambridge, MA), phospho-histone 2A.X (Ser139) (phospho-H2A.X) (1:100, Cell Signaling), proliferating cell nuclear antigen (PCNA) (1:250, Abcam), goat affinity purified polyclonal antibody galectin-3 (1:2000, R&D Systems, Minneapolis, MN) or appropriate controls. Slides were then incubated for 30 min with biotinylated goat anti-rabbit or rabbit anti-goat secondary antibody (Vector Labs, Burlingame, CA). Antibody binding was visualized using a DAB Peroxidase Substrate Kit (Vector Labs).
Joseph L.B., Heck D.E., Cervelli J.A., Composto G.M., Babin M.C., Casillas R.P., Sinko P.J., Gerecke D.R., Laskin D.L, & Laskin J.D. (2014). Structural Changes in Hair Follicles and Sebaceous Glands of Hairless Mice Following Exposure to Sulfur Mustard. Experimental and molecular pathology, 96(3), 316-327.
+ Open protocol
+ Expand
5

Protein Extraction from Tissue for Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extraction from tissue was performed by re-suspending frozen tissue in 0.5 mL of RIPA buffer (1% NP-40, 0.5% Deoxycholate, 0.1% SDS, 150 mM NaCl, 50 mM Tris plus protease and phosphatase inhibitors), and lysed using a tissue lyser (Qiagen) twice for 30 seconds at 20 Hz. Following lysis, samples were incubated on ice for 10 minutes, then spun down at 15,000 RCF for 5 minutes in 4°C. Supernatant was collected and stored in −80°C until immunoblotting. Antibodies used in this study: ATP-Citrate Lyase (Proteintech 15421–1-AP), Acyl-CoA Synthetase Family Member 2 (Cell Signaling Technology 3658S), Acetyl-CoA Carboxylase (Cell Signaling Technology 3676S), Fatty Acid Synthase (Cell Signaling Technology 3189S), Catalase (Cell Signaling Technology 14097S), Ribosomal Protein S6 (Cell Signaling Technology 2217S), IRDye800CW Goat Anti-Rabbit (LI-COR 926–32211). Immunoblots were developed using a LI-COR Odyssey Clx.
Zhao S., Jang C., Liu J., Uehara K., Gilbert M., Izzo L., Zeng X., Trefely S., Fernandez S., Carrer A., Miller K.D., Schug Z.T., Snyder N.W., Gade T.P., Titchenell P.M., Rabinowitz J.D, & Wellen K.E. (2020). Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate. Nature, 579(7800), 586-591.
+ Open protocol
+ Expand
6

Protein Extraction from Tissue for Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extraction from tissue was performed by re-suspending frozen tissue in 0.5 mL of RIPA buffer (1% NP-40, 0.5% Deoxycholate, 0.1% SDS, 150 mM NaCl, 50 mM Tris plus protease and phosphatase inhibitors), and lysed using a tissue lyser (Qiagen) twice for 30 seconds at 20 Hz. Following lysis, samples were incubated on ice for 10 minutes, then spun down at 15,000 RCF for 5 minutes in 4°C. Supernatant was collected and stored in −80°C until immunoblotting. Antibodies used in this study: ATP-Citrate Lyase (Proteintech 15421–1-AP), Acyl-CoA Synthetase Family Member 2 (Cell Signaling Technology 3658S), Acetyl-CoA Carboxylase (Cell Signaling Technology 3676S), Fatty Acid Synthase (Cell Signaling Technology 3189S), Catalase (Cell Signaling Technology 14097S), Ribosomal Protein S6 (Cell Signaling Technology 2217S), IRDye800CW Goat Anti-Rabbit (LI-COR 926–32211). Immunoblots were developed using a LI-COR Odyssey Clx.
Zhao S., Jang C., Liu J., Uehara K., Gilbert M., Izzo L., Zeng X., Trefely S., Fernandez S., Carrer A., Miller K.D., Schug Z.T., Snyder N.W., Gade T.P., Titchenell P.M., Rabinowitz J.D, & Wellen K.E. (2020). Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate. Nature, 579(7800), 586-591.
+ Open protocol
+ Expand
7

AMPK Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Briefly, the treated cells were washed twice with chilled PBS and the cell lysates were prepared in a lysis buffer [8 (link)]. The cell lysates were clarified at 14,000 g for 20 min at 4 °C. The protein concentrations were estimated by Bradford reagent.
Equal amount of proteins was resolved in SDS-PAGE. Following SDS-PAGE, the electro-blotted nitrocellulose membranes were reacted with relevant primary antibody specific to AMPK, phospho-AMPK (Thr172), ACC, phospho-ACC (Ser79), Fatty Acid Synthase and Actin (Cell Signaling Technology, Boston, MA). Specific signals were detected with enhanced chemiluminescence (Thermo scientific, USA) and the signal intensities were captured in Gel Doc™ XR+ Imaging System and analyzed using Image Lab™ 2.0 Software (BioRad, Hercules, CA).
Kudiganti V., Kodur R.R., Kodur S.R., Halemane M, & Deep D.K. (2016). Efficacy and tolerability of Meratrim for weight management: a randomized, double-blind, placebo-controlled study in healthy overweight human subjects. Lipids in Health and Disease, 15(1), 136.
+ Open protocol
+ Expand
8

Western Blot Analysis of HepG2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For Western blot analysis, HepG2 cells were lysed in the RIPA buffer supplemented with protease inhibitors (Thermo Scientific). Lysates were separated by SDS-PAGE and immunoblotted with antibodies as indicated. Western blot analysis was performed with the following antibodies: SERBP1 (Abcam, ab28481), Fatty acid synthase (Cell Signaling, 3180S), β-actin (Santa Cruz Biotechnology, sc-47778).
Lou B., Wu H., Ott H., Bennewitz K., Wang C., Poschet G., Liu H., Yuan Z., Kroll J, & She J. (2023). Increased circulating uric acid aggravates heart failure via impaired fatty acid metabolism. Journal of Translational Medicine, 21, 199.
+ Open protocol
+ Expand
9

Inhibition of VEGF-Induced Fibrosis Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
VEGF-inhibitor CBO-P11 was obtained from Calbiochem (San Diego, CA). BLM sulfate was obtained from Sigma-Aldrich (St. Louis, MO). Antibodies against Acetyl-CoA carboxylase (ACC), phospho-Acetyl-CoA carboxylase (Ser79) (pACC), Fatty Acid Synthase (FASN), AMP-activated protein kinase (AMPK), phosphor-AMP-activated protein kinase (pAMPK), alpha-smooth muscle actin (α-SMA), HRP-conjugated anti-rabbit IgG antibody and HRP-conjugated anti-mouse IgG antibody were obtained from Cell Signaling Technology (Danvers, MA). Collagen III (COL3A1) was obtained from Santa Cruz Biotechnology (Dallas, TX). Sterol regulatory element binding protein – 1c (SREBP-1c) and β-actin antibodies were obtained from Sigma-Aldrich (St. Louis, MO).
Kulkarni Y.M., Dutta S., Iyer A.K., Wright C.A., Ramesh V., Kaushik V., Semmes O.J, & Azad N. (2018). A Lipidomics Approach to Identifying Key Lipid Species Involved in VEGF-Inhibitor Mediated Attenuation of Bleomycin-Induced Pulmonary Fibrosis. Proteomics. Clinical applications, 12(3), e1700086.
+ Open protocol
+ Expand
10

Comprehensive Protein Analysis of Adipogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
SDS-PAGE and immunoblot analysis of samples were performed using the following antibodies: custom-made PDGF D (22 (link)), fatty acid synthase (Cell Signaling Technologies [CST]; catalog no.: 3180), C/EBPα (CST; catalog no.: 8178), peroxisome proliferator–activated receptor gamma (CST; catalog no.: 2435), perilipin (CST; catalog no.: 9349), FABP4 (CST; catalog no.: 2120), acetyl CoA carboxylase (CST; catalog no.: 3676), β-actin (CST; catalog no.: 4970), p-AKT (CST; catalog no.: 9275), AKT (CST; catalog no.: 9272), p-JNK (CST; catalog no.: 4668), JNK (CST; catalog no.: 9258), p-ERK1/2 (CST; catalog no.: 9101), ERK1/2 (CST; catalog no.: 9102), p-β-PDGFR Y751 (CST; catalog no.: 3161), p-β-PDGFR Y771 (CST; catalog no.: 3173), β-PDGFR (CST; catalog no.: 3169), vinculin (Sigma; catalog no.: V9131), ubiquitin (CST; catalog no.: 3936), ubiquitin-K48 (Abcam; catalog no.: ab140601), ubiquitin-K63 (Abcam; catalog no.: ab179434), HUWE1 (Abcam; catalog no.: ab70161), and streptavidin–horseradish peroxidase (Abcam; catalog no.: ab7403).
Pham T., Najy A.J, & Kim H.R. (2022). E3 ligase HUWE1 promotes PDGF D-mediated osteoblastic differentiation of mesenchymal stem cells by effecting polyubiquitination of β-PDGFR. The Journal of Biological Chemistry, 298(6), 101981.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!