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X omat 3000 ra film processor

Manufactured by Kodak
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The X-OMAT 3000 RA is a film processor designed for automated processing of radiographic films. It is a self-contained unit that provides consistent and reliable film development. The X-OMAT 3000 RA is capable of processing a variety of film sizes and can handle high-volume film processing needs.

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Lab products found in correlation

Cl xposure film, by Thermo Fisher Scientific (6 mentions) Immobilon western ecl detection buffers, by Merck Group (3 mentions) Bis tris sds page gel electrophoresis, by Thermo Fisher Scientific (2 mentions) Donkey anti goat igg secondary antibody, by R&D Systems (2 mentions) Western ecl detection solution, by Thermo Fisher Scientific (2 mentions) Chemidoc xrs system, by Bio-Rad (2 mentions) Anti p62, by Cell Signaling Technology (1 mentions) Anti lc3, by Cell Signaling Technology (1 mentions) Nupage 4 12 bis tris sds page, by Thermo Fisher Scientific (1 mentions) Supersignal west pico plus chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Superblock, by Thermo Fisher Scientific (1 mentions) Anti mbp, by Santa Cruz Biotechnology (1 mentions)

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X-Omat processor X-OMAT film X-Omat

6 protocols using x omat 3000 ra film processor

1

Quantitative Western Blot Analysis of sPDGFRβ in CSF

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The quantitative western blot analysis was used to detect sPDGFRβ in human CSF (ng/mL), as we previously reported.26, 27 Briefly, CSF samples and recombinant human PDGFRβ carrier‐free protein (Cat. No. 385‐PR‐100/CF, R&D Systems) were subjected to 4–12% Bis‐Tris SDS/PAGE gel electrophoresis (Thermo Scientific) for 2 h at 150 V and subsequently transferred to a nitrocellulose membrane. The membrane was blocked for 1 h with a superblock blocking buffer (Cat. No. 37537, Thermo Scientific). The membrane was incubated with a primary antibody for PDGFRβ (Cat. No. AF1042, R&D Systems) overnight, then incubated with donkey anti‐goat IgG secondary antibody (Cat. No. A15999, 1:5000 dilution, Thermo Scientific) for 1 h at room temperature, treated with Western ECL detection solution (Cat. No. 34075, Thermo Scientific), exposed to CL‐Xposure film (Cat. No. 34091, Thermo Scientific), and developed in an X‐Omat 3000 RA film processor (Kodak). Images were acquired, and densitometry analysis was performed using NIH Image J software.
Cowan R.P., Gross N.B., Sweeney M.D., Sagare A.P., Montagne A., Arakaki X., Fonteh A.N., Zlokovic B.V., Pogoda J.M, & Harrington M.G. (2021). Evidence that blood–CSF barrier transport, but not inflammatory biomarkers, change in migraine, while CSF sVCAM1 associates with migraine frequency and CSF fibrinogen. Headache, 61(3), 536-545.
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2

Autophagy Markers in Oligodendrocytes

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The autophagy markers were analyzed in oligodendrocytes 12 and 24 h after treatment with 1.5 mg/mL fibrinogen or 0.1 mg/mL fibrin, with or without mTOR activator MHY1485 (#500554, Calbiochem) or autophagy inhibitor VII (#534360, Calbiochem). Cells were lysed in RIPA buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 0.1% SDS, 1.0% NP-40, 0.5% sodium deoxycholate and Roche protease inhibitor cocktail). Samples were then subjected to NuPAGE 4-12% bis-tris-SDS-PAGE (ThermoFisher) and transferred to a nitrocellulose membrane. Membranes were blocked with SuperBlock (ThermoFisher), incubated with anti-p62 (Cell Signaling, #5114; 1:1,000) or anti-LC3 (Cell Signaling, #4108; 1:1,000) rabbit polyclonal antibodies, and then incubated with HRP-conjugated donkey anti-rabbit secondary antibody (ThermoFisher, #A16023; 1:5,000). Membranes were then treated with SuperSignal™ West Pico PLUS chemiluminescent substrate (#34580, ThermoFisher), exposed to CL-XPosure film (#34097, Thermo Scientific) and developed in a X-OMAT 3000 RA film processor (Kodak). Relative abundance of the LC3-II/I ratio was quantified against the loading control β-actin as described79 (link).
Montagne A., Nikolakopoulou A.M., Zhao Z., Sagare A.P., Si G., Lazic D., Barnes S.R., Daianu M., Ramanathan A., Go A., Lawson E.J., Wang Y., Mack W.J., Thompson P.M., Schneider J.A., Varkey J., Langen R., Mullins E., Jacobs R.E, & Zlokovic B.V. (2018). Pericyte degeneration causes white matter dysfunction in the mouse CNS. Nature medicine, 24(3), 326-337.
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3

Western Blot Protein Analysis

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All samples were lysed in RIPA buffer, subjected to SDS-Page gel electrophoresis and transferred to a nitrocellulose membrane. Membranes were blocked with 5% milk, incubated with primary antibody, incubated with the appropriate HRP-conjugated secondary antibody, treated with Immobilon Western ECL detection buffers (Millipore), exposed to CL-XPosure film (Thermo Scientific) and developed in a X-OMAT 3000 RA film processor (Kodak, Rochester, NY), or ChemiDoc XRS system from Bio-Rad.
Zhao Z., Sagare A.P., Ma Q., Halliday M.R., Kong P., Kisler K., Winkler E.A., Ramanathan A., Kanekiyo T., Bu G., Owens N.C., Rege S.V., Si G., Ahuja A., Zhu D., Miller C.A., Schneider J.A., Maeda M., Maeda T., Sugawara T., Ichida J.K, & Zlokovic B.V. (2015). Central role for PICALM in amyloid–β blood–brain barrier transcytosis and clearance. Nature neuroscience, 18(7), 978-987.
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4

Western Blot Protein Analysis

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All samples were lysed in RIPA buffer, subjected to SDS-Page gel electrophoresis and transferred to a nitrocellulose membrane. Membranes were blocked with 5% milk, incubated with primary antibody, incubated with the appropriate HRP-conjugated secondary antibody, treated with Immobilon Western ECL detection buffers (Millipore), exposed to CL-XPosure film (Thermo Scientific) and developed in a X-OMAT 3000 RA film processor (Kodak, Rochester, NY), or ChemiDoc XRS system from Bio-Rad.
Zhao Z., Sagare A.P., Ma Q., Halliday M.R., Kong P., Kisler K., Winkler E.A., Ramanathan A., Kanekiyo T., Bu G., Owens N.C., Rege S.V., Si G., Ahuja A., Zhu D., Miller C.A., Schneider J.A., Maeda M., Maeda T., Sugawara T., Ichida J.K, & Zlokovic B.V. (2015). Central role for PICALM in amyloid–β blood–brain barrier transcytosis and clearance. Nature neuroscience, 18(7), 978-987.
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5

Western Blot Analysis of Myelin Basic Protein

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White matter tissue (i.e., corpus callosum, internal capsule, cingulum, and external capsule) was lysed in RIPA buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 0.1% Sodium Dodecyl Sulfate (SDS), 1.0% NP-40, 0.5% sodium deoxycholate and Roche protease inhibitor cocktail). Samples were then subjected to bis-tris-SDS-PolyAcrylamide Gel Electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane. Membranes were blocked with 5% milk, incubated with anti-MBP (Santa Cruz, sc-13914; 1:1,000), and then incubated with HRP-conjugated donkey anti-goat secondary antibody (Invitrogen, A16005; 1:5,000). Membranes were then treated with Immobilon Western ECL detection buffers (Millipore), exposed to CL-XPosure film (Thermo Scientific) and developed in a X-OMAT 3000 RA film processor (Kodak).
Montagne A., Nikolakopoulou A.M., Zhao Z., Sagare A.P., Si G., Lazic D., Barnes S.R., Daianu M., Ramanathan A., Go A., Lawson E.J., Wang Y., Mack W.J., Thompson P.M., Schneider J.A., Varkey J., Langen R., Mullins E., Jacobs R.E, & Zlokovic B.V. (2018). Pericyte degeneration causes white matter dysfunction in the mouse CNS. Nature medicine, 24(3), 326-337.
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6

Quantitative Western Blot of sPDGFRβ in CSF

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The quantitative western blot analysis was used to detect sPDGFRβ in human CSF (ng/mL), as we previously reported.26 (link),27 (link) Briefly, CSF samples and recombinant human PDGFRβ carrier-free protein (Cat. No. 385-PR-100/CF, R&D Systems) were subjected to 4–12% Bis-Tris SDS/PAGE gel electrophoresis (Thermo Scientific) for 2 h at 150 V and subsequently transferred to a nitrocellulose membrane. The membrane was blocked for 1 h with a superblock blocking buffer (Cat. No. 37537, Thermo Scientific). The membrane was incubated with a primary antibody for PDGFRβ (Cat. No. AF1042, R&D Systems) overnight, then incubated with donkey anti-goat IgG secondary antibody (Cat. No. A15999, 1:5000 dilution, Thermo Scientific) for 1 h at room temperature, treated with Western ECL detection solution (Cat. No. 34075, Thermo Scientific), exposed to CL-Xposure film (Cat. No. 34091, Thermo Scientific), and developed in an X-Omat 3000 RA film processor (Kodak). Images were acquired, and densitometry analysis was performed using NIH Image J software.
Cowan R.P., Gross N.B., Sweeney M.D., Sagare A.P., Montagne A., Arakaki X., Fonteh A.N., Zlokovic B.V., Pogoda J.M, & Harrington M.G. (2021). Evidence that blood–CSF barrier transport, but not inflammatory biomarkers, change in migraine, while CSF sVCAM1 associates with migraine frequency and CSF fibrinogen. Headache, 61(3), 536-545.
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