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Acridine orange

Manufactured by Maclin Biochemical Technology
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Sourced in China

Acridine orange is a fluorescent dye used for staining and visualization of nucleic acids, including DNA and RNA, in biological samples. It has the ability to intercalate with double-stranded DNA and bind to single-stranded DNA and RNA. Acridine orange emits green fluorescence when bound to DNA and red fluorescence when bound to RNA, allowing for the differentiation between these molecular components.

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Lab products found in correlation

Sodium chloride (nacl), by Maclin Biochemical Technology (1 mentions) Ammonium hydroxide solution, by Maclin Biochemical Technology (1 mentions) Triethylamine, by Maclin Biochemical Technology (1 mentions) Citric acid, by Maclin Biochemical Technology (1 mentions) Formamide, by Maclin Biochemical Technology (1 mentions) Anhydrous ethanol, by Sinopharm (1 mentions) Tetraethyl orthosilicate, by Maclin Biochemical Technology (1 mentions) 3 aminopropyl triethoxysilane, by Maclin Biochemical Technology (1 mentions) N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Maclin Biochemical Technology (1 mentions) N hydroxysuccinimide, by Maclin Biochemical Technology (1 mentions) Toluene, by Sinopharm (1 mentions) Methanol, by Sinopharm (1 mentions) Acetone, by Sinopharm (1 mentions) Lsm 700, by Zeiss (1 mentions)

2 protocols using acridine orange

1

Facile Synthesis of Fluorescent Silica Nanoparticles

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Citric acid
(C6H8O7, AR grade), formamide (CH3NO, AR grade), acridine orange (C17H19N3, biotechnology grade), tetraethyl orthosilicate (C8H20O4Si, GC grade), ammonium hydroxide
solution (NH3·H2O, AR grade), triethylamine
(C6H15N, AR grade), glutaric anhydride (C5H6O3, 98%), sodium chloride (NaCl, AR
grade), (3-aminopropyl)triethoxysilane (C9H23NO3Si, 99%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (C8H17N3·HCl, 98.5%), and N-hydroxysuccinimide (C4H5NO3, 99%)
were purchased from Shanghai Macklin Biochemical Co., Ltd. Ethanol
anhydrous (C2H6O, AR grade), methanol (CH4O, AR grade), acetone (C3H6O, AR grade),
and toluene (C7H8, AR grade) were purchased
from Sinopharm Chemical Reagent Co., Ltd. The other reagents used
were of AR grade without further purification, and ultrapure water
was used in all experiments. The miR-92a-3p, DNA probe, amino-modified
DNA (NH2-DNA) probe, and unmatched targets were purchased
from Shanghai Genepharma Co., Ltd. Their sequences are listed in Table S1 of the Supporting Information. The human
serum was isolated from the healthy sample from the Second Hospital
of Shandong University. The apparatus used are given in the Supporting Information.
Sun Z., Tong Y., Zhou X., Li J., Zhao L., Li H., Wang C., Du L, & Jiang Y. (2021). Ratiometric Fluorescent Biosensor Based on Forster Resonance Energy Transfer between Carbon Dots and Acridine Orange for miRNA Analysis. ACS Omega, 6(49), 34150-34159.
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2

Staining S. marcescens Biofilm Using Confocal Microscopy

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The S. marcescens NJ01 strain was cultured as described above. Planktonic bacteria on the circular coverslips were washed with PBS after incubation. Subsequently, acridine orange (5 μL, Shanghai Macklin Biochemical Technology Co., Ltd., China) and ethidium bromide (5 μL, Aladdin, Shanghai, China) was added dropwise to the glass slide to stain the biofilm for 5 min. Excess dye was washed using PBS, and the coverslips were observed using a confocal laser scanning microscopy (CLSM, Zeiss LSM 700, Carl Zeiss, Jena, Germany) (42 (link)).
Yin L., Zhang P.P., Wang W., Tang S., Deng S.M, & Jia A.Q. (2022). 3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing. Microbiology Spectrum, 10(5), e01829-22.
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