BMSCs were grown on cover glasses and treated with curculigoside and Ti. After that, 1 ml of 0.5% Triton X-100 was added into each well for cell permeabilization. And 3% BSA was used to block the reaction; then, BMSCs were incubated with the primary antibody against F-actin (Abcam, USA) overnight at 4°C and further incubated with the secondary antibody (Abcam, USA). After incubation and washing with PBS, the cell nuclei were stained with DAPI. Cells were observed under an inverted fluorescence microscope (Ts2-FC, Nikon, Japan).
Primary antibody against f actin
Manufactured by Abcam
Sourced in United States
This primary antibody is designed to detect F-actin, a key component of the cytoskeleton in eukaryotic cells. It can be used in various immunoassay applications to visualize and study the organization and dynamics of the actin cytoskeleton.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using primary antibody against f actin
1
Cytoskeletal Dynamics in BMSCS
2
Fluorescent Labeling of F-Actin in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded in 24-well plates and fixed in 4% paraformaldehyde (Biosesang) for 10 min at 22–25 °C and permeabilized in PBS with 0.2% Triton X-100 (PBST; Sigma-Aldrich) for 10 min. The cells were washed in PBST; the plates were blocked with 2.5% BSA in PBST for 30 min and incubated with the primary antibody against F-actin (1:200; Abcam, Waltham, MA, USA). The plates were washed and incubated with Alexa Fluor-488-conjugated secondary antibodies for 2 h and stained with 4′,6-diamidino-2-phenylindole. The stained plates were analyzed using Olympus IX71 microscope (Olympus).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!