Frozen sections were fixed with 4% paraformaldehyde/phosphate-buffered saline and stained with the following antibodies conjugated with Alexa 488 or FITC or Alexa 633: anti-CD3 (BD Pharmingen, San Diego, CA, clone 17A2), anti-CD4 (eBioscience, San Diego, CA, clone RM4-5), anti-γδ TCR (BD Pharmingen, clone GL3), anti-Vγ3 TCR (BD Pharmingen, clone 536), or anti-Ly6G/Gr-1 (Beckman Coulter, Miami, FL, clone RB6-8C5). Formalin-fixed paraffin sections were treated with citrate buffer for antigen retrieval and incubated with anti-Camp (Abcam, Cambridge, UK) or anti-β3 defensin (Alpha Diagnostic, San Antonio, TX) followed by Alexa 488-conjugated donkey anti-rabbit antibody (Life Technologies). DAPI or PI was used for nuclear staining. Images were acquired using a TCS SP5 Tandem Scanner or an FV1000 confocal microscope (Olympus, Tokyo, Japan). When quantifying cells that expressed a particular marker, at least six fields were analyzed per skin section.
Anti γδ tcr
Manufactured by BD
The Anti-γδ TCR is a laboratory reagent used for the detection and analysis of γδ T cells. It binds specifically to the γδ T cell receptor, allowing for the identification and quantification of this cell population within a sample.
Automatically generated - may contain errors
Lab products found in correlation
Alexa 488 conjugated donkey anti rabbit antibody, by Thermo Fisher Scientific (2 mentions)
Anti vγ3 tcr, by BD (2 mentions)
Anti ly6g gr 1, by Beckman Coulter (2 mentions)
Anti camp, by Abcam (2 mentions)
Anti β3 defensin, by Alpha Diagnostic (2 mentions)
Tcs sp5 tandem scanner, by Olympus (2 mentions)
Anti cd4, by Thermo Fisher Scientific (2 mentions)
Anti cd3, by BD (2 mentions)
Fv1000 confocal microscope, by Olympus (2 mentions)
2 protocols using anti γδ tcr
1
Immunofluorescence Analysis of Skin Immune Cells
2
Multicolor Immunofluorescence Staining of Skin Sections
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