Ck2 inhibitor cx 4945

Manufactured by Selleck Chemicals

CK2 inhibitor CX-4945 is a potent and selective small-molecule inhibitor of the protein kinase CK2. It is a white to off-white crystalline powder that is soluble in DMSO and other organic solvents. CX-4945 is commonly used as a research tool in biochemical and cell-based studies to investigate the role of CK2 in various cellular processes.

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Lab products found in correlation

Chk1 ps345, by Cell Signaling Technology (1 mentions) 5 ethynyl 2 deoxyuridine (edu), by Thermo Fisher Scientific (1 mentions) Proliferating cell nuclear antigen (pcna), by Abcam (1 mentions) Gapdh, by Abcam (1 mentions) Chk1 ps296, by Cell Signaling Technology (1 mentions) Pha 767491, by Selleck Chemicals (1 mentions) Chk1 inhibitor ucn01, by Merck Group (1 mentions) Azd1152, by Selleck Chemicals (1 mentions) P276 00, by Selleck Chemicals (1 mentions) Isrib, by Merck Group (1 mentions)

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CX-4945 (45 citations)

2 protocols using ck2 inhibitor cx 4945

Antibody and Inhibitor Characterization for DNA Damage Response

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Antibodies were as follows: MCM4 (Cell Signaling, catalog no. 3228) (used at 1:1,000), CHK1 (Cell Signaling, catalog no. 2360) (used at 1:1,000), CHK1 pS345 (Cell Signaling, catalog no. 2348) (used at 1;1000), CHK1 pS296 (Cell Signaling, catalog no. 2349) (used at 1:1,000), GAPDH (Abcam, catalog no. 8245) (used at 1:10,000), IdU (Becton Dickinson, catalog no. 347580) [used 1:300 for immunofluorescence (IF)], CldU (Abcam, catalog no. 6326) (used 1:100 for IF), PCNA (Abcam, catalog no. ab201674) (used 1:1,000 for STORM), RIF1 [Bethyl, catalog no. A300-568A; used 1:1,000 for Western blot (WB) and 1:500 for PLA], PP1alpha (Thermo, catalog no. 43-8100, used 1:1,000 for PLA; Cell Signaling, catalog no. 2582, used 1:500 for WB). RIF1 pSer2205 antibody was custom-made by Bethyl by injecting rabbits with KVRRV(pS)FADPI peptide and subsequent purifications. Other materials included ATR inhibitor AZD6738 (AstraZeneca); CHK1 inhibitor UCN01 (Sigma); CHK1 inhibitor AZD7762 (AstraZeneca); CDC7 inhibitor PHA-767491, Aurora B inhibitor AZD1152, PKA inhibitor H89, CK2 inhibitor CX-4945, CDK1 inhibitor Ro-3306, and CDK1/4/9 inhibitor P276-00 (all from Selleckchem); CDK2i CVT-313 (Santa Cruz); EdU (Invitrogen); IdU (Sigma); and CldU (MP Biomedical).

Moiseeva T.N., Yin Y., Calderon M.J., Qian C., Schamus-Haynes S., Sugitani N., Osmanbeyoglu H.U., Rothenberg E., Watkins S.C, & Bakkenist C.J. (2019). An ATR and CHK1 kinase signaling mechanism that limits origin firing during unperturbed DNA replication. Proceedings of the National Academy of Sciences of the United States of America, 116(27), 13374-13383.

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Neurotransmitter-Induced Cell Signaling Assay

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Cells were differentiated as described and treated with NE (Sigma) at a concentration of 1μM for 18 hours, unless otherwise stated, before harvesting the cells. When indicated other compounds were used: PERK inhibitor (GSK2606414, Millipore) at 500 nM, PERK activator (CCT020312, Millipore) at 500 nM, OGT inhibitor (OSMI1, Abcam) at 50 μM, CK2 inhibitor (CX4945, Selleck Chemicals) at 200 nM, PKAc inhibitor (H89, Selleck Chemicals) at 10 μM and eIF2α inhibitor (ISRIB, Sigma) at 250 nM.

Latorre-Muro P., O’Malley K.E., Bennett C.F., Perry E.A., Balsa E., Tavares C.D., Jedrychowski M., Gygi S.M, & Puigserver P. (2021). A Cold Stress Inducible PERK/OGT Axis Controls TOM70-Assisted Mitochondrial Protein Import and Cristae Formation. Cell metabolism, 33(3), 598-614.e7.

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