Tim3 apc fire750

Manufactured by BioLegend

TIM3-APC/Fire750 is a fluorochrome-conjugated antibody that binds to the T-cell immunoglobulin and mucin domain-containing protein 3 (TIM3). TIM3 is an inhibitory receptor expressed on various immune cell types, including T cells, NK cells, and dendritic cells. The APC/Fire750 fluorochrome allows for the detection and analysis of TIM3-expressing cells using flow cytometry.

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TIM3-APC (5 citations)

2 protocols using tim3 apc fire750

Multiparameter Flow Cytometry for Immune Cell Analysis

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Fluorochrome-conjugated antibodies were used to select and sort cell subsets, analyze T cell phenotypes, and to determine cytokine production. The following antibodies were used in this study, separated by manufacturer (clone is indicated in parentheses). Biolegend: Ki67-PacificBlue (16A8), CD62L-BV510 (MEL-14), CD73-BV605 (TY/11.8), CD44-FITC (IM7), PD1-PEDazzle594 (RMP1–30), TIGIT-PECy7 (1G9), LAG3-BV785 (C9B7W), IFNγ (XMG1.2), TNFα-PECy7 (MP6-XT22), SATB1-AlexaFluor594 (O96C6), TIM3-APC/Fire750 (B8.2C12), OX40-BV711 (OX-86), OX40L-PECy7 (RM134L), Tim3-PerCP/Cy5.5 (B8.2C12), CD8-FITC (53–6.7), CD90.2-PECy7 (30H12), CD90.2-PerCP/Cy5 (53–2.1), CD4-APCCy7 (RM4–5). || BD Biosciences: CD90.2-BUV395 (53–2.1), CD4-BUV496 (GK1.5), CD19-BUV661 (1D3), CD11c-BUV661 (N418), F4/80-BUV661 (T45–2342), NK1.1BUV661 (PK136), TER119-BUV661 (TER-119), CD127-BUV737 (SB/199), CD8-BUV805 (53–6.7), FR4-BV421 (12A5), CTLA4-APCR700 (UC10–4F10–11), NK1.1-eFluor450 (PK136), Ter-119-eFluor450 (Ter119), Rorγt-BV650 (Q31–378), CD62L-BV605 (MEL-14). || Invitrogen: FoxP3-AlexaFluor532 (FJK-16s), CD44-BUV737 (IM7), PD1-SB780 (J43), TOX-eFluor660 (TXRX10), EOMES-PerCP/eFluor710 (Dan11mag), F4/80-eFluor450 (BM8), CD49b-eFluor450 (DX5), CD11c-eFluor450 (N418), PD1-PerCPe710 (J43). || Santa Cruz Biotechnologies: NFATc1-AlexaFluor488 (7A6), CD30L-AlexaFluor680 (RM153).

Pollard J., Hynes G., Yin D., Mandal M., Gounari F., Alegre M.L, & Chong A. (2023). Pregnancy programs epigenetic and transcriptional exhaustion in memory CD8+ T cells. Research Square.

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Multiparametric Flow Cytometry of Tumor Cells

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Single-cell suspensions of tumors were prepared, and flow cytometry was carried out as mentioned earlier [45 (link), 46 (link)]. Cells were stained with the following antibodies obtained from BioLegend: CD16/32 (clone 93, catalog No. 103132), Zombie UV™ Fixable Viability Kit (catalog No. 100752), CD45-PerCP/Cy5.5 (clone 30-F11, catalog No. 103132), CD45-APC/Cy7 (clone 30-F11, catalog No. 103116), CD11b-FITC (clone M1/70, catalog No. 101206), SIRPα-PE (clone BM8, catalog No. 123122), Gr-1-PerCP/Cy5.5 (clone RB6-8C5, catalog No. 108428), F4/80-AF647 (clone N418, catalog No. 117318),CD11c-PE/Cy7 (clone N418, catalog No. 117318), I-A/I-E-AF700 (clone M5/114.15.2, catalog No. 107622), CD86-BV421 (clone GL-1, catalog No. 105032), CD206-BV605 (clone C068C2, catalog No. 141721), CD8a-AF700 (clone 53-6.7, catalog No. 100730), CD8-BV510 (clone 53-6.7, catalog No. 100752), TNF-α-BV421 (clone MP6-XT22, catalog No. 506328), IFN-γ-PE (clone XMG1.2, catalog No. 505808), CD39-PE/Cy7 (clone Duha59, catalog No. 143806), Tim-3-APC/Fire™ 750 (clone RMT3-23, catalog No. 119738), PD-1-BV605 (clone 29 F.1A12, catalog No. 135220). The next antibodies were obtained through eBioscience: Mouse Regulatory T Cell Staining Kit #2 (clone FJK-16s, catalog No. 88-8118-40). FlowJo program (Tree Star Inc.) was utilized to examine the findings that were acquired through a BD LSRFortessa Flow Cytometer.

Ji K., Zhang Y., Jiang S., Sun L., Zhang B., Hu D., Wang J., Zhao L., Wang P, & Tao Z. (2023). SIRPα blockade improves the antitumor immunity of radiotherapy in colorectal cancer. Cell Death Discovery, 9, 180.

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