Media for cell culture

Media for cell culture were purchased from Gibco BRL (Rockville, MD, USA). Fetal bovine serum and horse serum were from Hyclone (Logan, UT, USA) which inactivated at 55℃ for 30 min for use. HEPES (acid), glucose, NaHCO₃, NaCl, KCl, MgCl₂, CaCl₂, NaOH, phenol red, trypsin, cytosine arabinoside, epidermal growth factor, sucrose, ascorbate, NMDA, and trolox were purchased from Sigma-Aldrich. Apocynin, AEBSF, and N-benzyloxycarbonyl-Val-Ala-Aspfluoromethylketone (z-VAD-FMK) were obtained from Calbiochem Corporation (San Diego, CA, USA). BDNF (Upstate Biotechnology, USA), Aβ_1-42 (Bachem Americas, Inc. Torrance, CA, USA), mitotempol (Abcam, Cambridge, UK), and mitoquinone (Biovision, Milpitas, CA, USA) were used.

Media for cell culture were purchased from Gibco BRL (Rockville, MD, USA). HEPES (acid), glucose, NaHCO₃, NaCl, KCl, MgCl₂, CaCl₂, NaOH, phenol red, trypsin, cytosine arabinoside, epidermal growth factor, sucrose, ascorbate, cycloheximide, N-acetylcysteine, Trolox, and ascorbic acid were purchased from Sigma-Aldrich. N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (z-VAD-FMK) and MK-801 (RBI, USA) were purchased. Fetal bovine and horse sera from Hyclone (Logan, UT, USA) were inactivated by keeping at 55℃ for 30 min before use.