Clarus 600 ms

The crude oils obtained from the n-hexane fraction of S. edelbergii was profiled using gas chromatography-mass spectrometry (GC-MS) analysis, coupled with a Perkin-Elmer Clarus 600 GC Scheme, which was fitted with Rtx-5MS, a very small capillary column (30 m × 0.25 mm I.D × 0.25 μm with film thickness; up to 260 °C), fixed to a Perkin-Elmer Clarus 600 MS. Ultra-high purity of helium (99.99%) was administrated as a carrier gas at a constant maintained flow rate of 1.0 mL/min. The injection temperature was 260 °C, while the temperature transfer line was 270 °C. The ion source was observed with a temperature of 280 °C. Furthermore, the ionizing energy (IE) was 70 eV. Moreover, the electron multiplier (EM) voltage was accessed from auto-tune. All data were obtained by assembling the full-scan mass spectra within the scan range of 45–550 a.m.u. The sample (at a concentration of 1 μL) was injected with a split ratio of 10:1. Moreover, the oven temperature was fixed for 1 min at 60°C and a specific rate of temperature of 4°C/min up to 260°C was maintained and fixed for 4 min. The system took 50 min to complete its total run [23 (link)].

GC/MS (Perkin Elmer, Clarus 680 GC coupled to a Clarus 600 MS) analysis was performed for the detection of major compounds present in various extracts of T. ornata. Column used in GC/MS was Elite-5MS (30.0 m, 0.25 mmID, and 250 μmdf). Carrier gas used was helium at a constant flow rate of 1 mL/min. EI mode with electron energy set at 70 eV was used. 1 μL of extract diluted with methanol was injected into GC/MS and the compounds were identified based on the molecular structure, molecular mass, and calculated fragment ratio of resolved spectra with that of mass spectra available from the library. Spectral data were interpreted using the database of National Institute Standard And Technology (NIST).