Murine renal tissues were surgically resected and dispersed in RPMI 1640 containing 5% FBS and 0.1% collagenase (Sigma-Aldrich) at 37°C for 30 min, followed by progressive sieving to obtain single-cell suspensions. To analyze the inflammatory response of renal macrophages, CD11b+/F4/80high renal macrophages were sorted from nephritic single-cell suspensions using a FACSAria (BD Biosciences) with FITC-labeled anti-F4/80 and PE-labeled anti-CD11b (BD Biosciences).
Pe labeled anti cd11b
Manufactured by BD
Sourced in United States
PE-labeled anti-CD11b is a fluorescently-labeled antibody that binds to the CD11b cell surface antigen. CD11b is a marker commonly expressed on myeloid cells, including monocytes, macrophages, and neutrophils. This antibody can be used to identify and analyze these cell types using flow cytometry or other immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Collagenase, by Merck Group (1 mentions)
Fitc labeled anti f4 80, by BD (1 mentions)
Facsaria, by BD (1 mentions)
Apc labeled anti cd3, by BD (1 mentions)
Fitc labeled anti cd11c, by BD (1 mentions)
Pe labeled anti foxp3, by Thermo Fisher Scientific (1 mentions)
Fitc labeled anti cd8, by BD (1 mentions)
Pe labeled anti cd4, by BD (1 mentions)
Fitc labeled anti igg1, by BD (1 mentions)
Fitc labeled anti cd4, by Thermo Fisher Scientific (1 mentions)
Cellquest pro software, by BD (1 mentions)
Accuri c6, by BD (1 mentions)
Fitc labeled anti cd14, by BD (1 mentions)
4 protocols using pe labeled anti cd11b
1
Isolation and Analysis of Renal Macrophages
2
Immune Cell Profiling in MRL.Fas(lpr) Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spleens were isolated from MRL.Faslpr mice at 25 weeks of age, and immune cell composition was analyzed by flow cytometry. Spleen cells were stained with the following monoclonal antibodies in 0.5% BSA/PBS at 4°C for 15 min: FITC-labeled anti-B220, APC-labeled anti-CD3, PE-labeled anti-CD4, FITC-labeled anti-CD8, FITC-labeled anti-CD11c, PE-labeled anti-CD11b, APC-labeled anti-CD138, FITC-labeled anti-IgG1 (BD Biosciences, San Jose, CA, USA), FITC-labeled anti-CD4, and PE-labeled anti-Foxp3 (eBioscience). Data were collected using FACSCalibur and analyzed with CellQuest Pro software (BD Biosciences).
3
Phenotyping of HL60 and Neutrophils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 1 × 106 #/ml HL60 cells or neutrophils were washed with PBS three times, blocked with 2% BSA, and stained, respectively, with PE-labeled anti-CD11a, PE-labeled anti-CD11b, and PE-labeled anti-CD162 antibodies (BD Bioscience, USA) for 30 min at 4°C in the dark as described previously (51 (link)). Then, the cells were washed three times with PBS and analyzed by flow cytometry (BD Accuri C6, BD, USA) with a minimum acquisition of 10,000 events.
4
Monocyte-Derived Macrophage Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PBMCs were isolated as described previously (Tamgue et al., 2019 (link)). For the generation of MDMs, PBMCs were plated in 12- or 96-well tissue culture plates (Corning Costar®, Cambridge, MA) at a density of 15 × 106 or 1,5 × 106 cells per well, respectively, and incubated (37 °C; 5% CO2; and 70% RH) for 2 h to allow monocytes to adhere. Non-adherent cells were discarded and adherent monocytes were given a gentle wash with PBS and then incubated in X-VIVO™ 15 serum-free hematopoietic medium (supplemented with 1% penicillin G/streptomycin) for 7 days to allow for the differentiation of monocytes into MDMs. X-VIVO™ 15 serum-free hematopoietic medium was changed on day 4. On day 7, X-VIVO™ 15 was removed, MDMs were washed once with PBS and complete growth medium (RPMI 1640 medium supplemented with 10% FCS, 2 mm l -glutamine, and 1% penicillin G/streptomycin. All purchased from Life Technologies™, Carlsbad, CA, United States) added for downstream experiments. MDMs purity was assessed by fluorescence-activated cell sorting (FACS) analysis using a PE–labeled anti-CD11b, PerCP-labeled anti–HLA-DR, FITC-labeled anti-CD14, and APC-labeled anti-CD3 monoclonal antibodies (All purchased from BD Biosciences™ CA, United States). MDMs purity was more than 95%.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!