Visionpro

Culture optical densities were recorded at a wavelength of 565 nm (OD₅₆₅) using an Evolution 300 UV-vis spectrophotometer controlled by Thermo Scientific™ VISIONpro™ software (Thermo Fisher Scientific, Hemel Hempstead, UK). Cellular magnetic response (C_mag) values were determined by OD₅₆₅ measurements using a purpose-built magnetic measurement system mounted within the spectrophotometer as described elsewhere.^{22 (link)}C_mag values greater than “1” indicate the presence of the magnetic cells.

Spectrometric
analysis for the initial and degradation dye samples are carried out
using an Evolution 300 UV–vis spectrophotometer equipped with
a long-lifetime xenon flash lamp and extended wavelength-range silicon
photodiode detectors. VISIONpro, Thermo Scientific software, was utilized
as a control and data manipulation package for scanning for sample
identification, method development, quantitative analysis, etc. The
output is taken as samples of size 25 mL each from the photocatalytic
reactor every hour and filtered using a 0.22 μm Durapore PVDF
membrane filter unit and stored in 15 mL Graduated Centrifuge tubes
(Tarsons) in dark containers. The test process lasts for 5 h until
complete degradation where each step is repeated, and the samples
are stored for further analysis. For the analysis of the absorbance
of the samples, 1 mL of samples is analyzed through an UV–vis
spectrophotometer. Quantification of the dye was performed at different
wavelengths of λ = 596 nm for RB-4 dye, λ = 661 nm for
the industrial dye sample, and wavelengths of {λ = (543 nm),
(624 nm), (560 nm), and (589 nm)} for various mentioned industrial
reactive dyes that form the part of the effluent mixture.