Transscript one step gdna removal and cnda synthesis supermix

Uterine tissues were treated with TRIzol (1 mL/100 mg) and RNA, was extracted as previously described (64 (link)). In brief, TRIzol-treated solution was treated with 200 μL chloroform and 500 μL isopropanol and washed with 75% DEPC water-diluted alcohol. After dissolving with DEPC water, RNA was reversely transcribed into cDNA using TransScript One-Step gDNA Removal and cNDA Synthesis SuperMix (Transgen Biotech, Beijing, China). SYBR green master (Roche, Germany) with mouse specific primer in a StepOnePlus apparatus (Applied Biosystems, Foster City, CA, USA) was performed. The oligonucleotides used are shown in Table S1. 2^-△△Ct quantification methods were used and GAPDH served as an endogenous control.

Uterine tissues were treated with TRIzol (1 mL/100 mg) and RNA, was extracted as previously described (64 (link)). In brief, TRIzol-treated solution was treated with 200 μL chloroform and 500 μL isopropanol and washed with 75% DEPC water-diluted alcohol. After dissolving with DEPC water, RNA was reversely transcribed into cDNA using TransScript One-Step gDNA Removal and cNDA Synthesis SuperMix (Transgen Biotech, Beijing, China). SYBR green master (Roche, Germany) with mouse specific primer in a StepOnePlus apparatus (Applied Biosystems, Foster City, CA, USA) was performed. The oligonucleotides used are shown in Table S1. 2^-△△Ct quantification methods were used and GAPDH served as an endogenous control.