Sc 7314

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-7314 is a laboratory equipment product from Santa Cruz Biotechnology. It is designed for scientific research purposes. The core function of Sc-7314 is to enable researchers to perform specific laboratory tasks and analyses.

Automatically generated - may contain errors

Lab products found in correlation

Ab35654, by Abcam (1 mentions) A21202, by Thermo Fisher Scientific (1 mentions) A21206, by Thermo Fisher Scientific (1 mentions) A10037, by Thermo Fisher Scientific (1 mentions) A 11057, by Thermo Fisher Scientific (1 mentions) Sc 15334, by Santa Cruz Biotechnology (1 mentions) Lsm 800 confocal laser scanning microscope, by Zeiss (1 mentions) A10042, by Thermo Fisher Scientific (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Ultravision lp large volume detection system hrp polymer, by Thermo Fisher Scientific (1 mentions) Ab68477, by Abcam (1 mentions) Ab137550, by Abcam (1 mentions)

3 protocols using sc 7314

Immunofluorescence Analysis of Gut Microbiota

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bacteria and cells were fixed in 3.7% formaldehyde (v/v) in PBS for 10 min at room temperature or in methanol/acetone (1:1) for 4 min on ice for mucin staining. Samples were blocked and permeabilized (if required) in 0.5% bovine serum albumin (w/v) and 0.1% Triton X-100 (v/v) in PBS for 20 min. Specimens were incubated in primary antibodies for 60 min at room temperature. The following antibodies were used in this study: goat anti-E. coli (1:400; ab13627, Abcam), mouse anti-E. coli LPS (1:200; ab35654, Abcam), rabbit anti-R. gnavus NanH (1:200) (Owen et al., 2017 (link)), rabbit anti-L. reuteri CmbA (1:250) (Etzold et al., 2014 (link)), rabbit anti-MUC2 (1:250; sc-15334, Santa Cruz Biotechnology), mouse anti-MUC2 (1:250; sc-7314, Santa Cruz Biotechnology) and rabbit anti-occludin (1:20; 40-4700, Invitrogen). For detection, samples were incubated in donkey anti-rabbit, donkey anti-mouse or donkey anti-goat IgG conjugated with Alexa Fluor 488 or Alexa Fluor 568 (1:400; A10037, A10042, A11057, A21206, A21202, Invitrogen) for 30 min. Actin and nucleic acids were stained with fluorescein isothiocyanate-conjugated phalloidin and 4′,6-diamidino-2-phenylindole (DAPI), respectively. Samples were mounted in Vectashield (Vector Laboratories) and analysed using an Axioimager fluorescent light or LSM800 confocal laser-scanning microscope (Zeiss).

McGrath C.J., Laveckis E., Bell A., Crost E., Juge N, & Schüller S. (2022). Development of a novel human intestinal model to elucidate the effect of anaerobic commensals on Escherichia coli infection. Disease Models & Mechanisms, 15(4), dmm049365.

+ Open protocol

+ Expand

Histological Analysis of Colonic Damage in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols

The middle sections of the distal colons of each rat were fixed in 4% neutral buffered formalin (NBF) and embedded in paraffin, and 3 μm sections were obtained. The slides were stained with H&E or AB. To estimate colonic damage, the slides stained with H&E were observed by a phase contrast microscope (LEICA ICC50, Wetzlar, Germany) and scored according to the parameters shown in Supplementary Table S1^{45 (link)}. Goblet cells in crypts were counted with the AB-stained specimens. For IHC, the antigen was retrieved using Tris-EDTA buffer (pH 9.0) and the detection procedure was performed using an UltraVision LP Large Volume Detection System: HRP Polymer (Thermo Scientific) according to the manufacturer’s instructions. The primary antibody against MUC2 (sc-7314, Santa Cruz, Dallas, TX, USA) was used, and the images obtained from the phase contrast microscope (LEICA ICC50) were analysed with ImageJ.

Ma S., Yeom J, & Lim Y.H. (2020). Dairy Propionibacterium freudenreichii ameliorates acute colitis by stimulating MUC2 expression in intestinal goblet cell in a DSS-induced colitis rat model. Scientific Reports, 10, 5523.

+ Open protocol

+ Expand

Selenium Compounds in Intestinal Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium selenite (Na₂SeO₃) and polyethylene glycol 6000 were purchased from Chengdu Cologne Chemical Co., Ltd., ammonium molybdate ((NH₄)₂MoO₄) were purchased from Tianjin No. 4 Chemical Reagent Factory, glutathione was purchased from Qingdao high tech Industrial Park Haibo Biotechnology Co., Ltd., DSS purchased from Shanghai Sangon Bioengineering Co., Ltd., LPS (L2880, from Escherichia coli O55:B5) was purchased from Sigma.
The following antibodies were used in this study: monoclonal rabbit anti-TLR4 (14358S, Cell Signaling Technology), monoclonal rabbit anti–NF–κB p65 (8242, Cell Signaling Technology), monoclonal rabbit anti-p–NF–κB (Ser536) (3033T, Cell Signaling Technology), monoclonal rabbit anti-IκBα (4814, Cell Signaling Technology), monoclonal mouse anti-β-Actin (3700, Cell Signaling Technology), polyclonal rabbit anti-Nrf2 (ab137550, Abcam), monoclonal rabbit anti-Keap1 (8047S, Cell Signaling Technology), polyclonal rabbit anti-NQO1 (ET1702-50, Huabio), polyclonal rabbit anti–HO–1 (ab68477, Abcam), monoclonal mouse anti-claudin-1 (sc-166338, Santa Cruz Biotechnology), monoclonal mouse anti-MUC-2 (sc-7314, Santa Cruz Biotechnology), polyclonal rabbit anti-occludin (27260-1-AP, Proteintech), polyclonal rabbit anti-ZO-1 (21773-1-AP, Proteintech).

Guo H., Guo H., Xie Y., Chen Y., Lu C., Yang Z., Zhu Y., Ouyang Y., Zhang Y, & Wang X. (2022). Mo3Se4 nanoparticle with ROS scavenging and multi-enzyme activity for the treatment of DSS-induced colitis in mice. Redox Biology, 56, 102441.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!