Matrine

Manufactured by Yuanye Bio-Technology

Sourced in China

Matrine is a natural compound extracted from the plant Sophora flavescens. It is a white crystalline powder with a molecular formula of C₁₅H₂₄N₂O. Matrine is commonly used as a laboratory reagent for research purposes.

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Lab products found in correlation

5 protocols using matrine

Matrine Regulates Autophagy in Cancer Cells

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Matrine was purchased from Shanghai Yuanye Biotechnology Co., Ltd., (purity 98%). DMEM was obtained from Gibco (Thermo Fisher Scientific, Inc.). Penicillin-streptomycin was purchased from Beijing Solarbio Science and Technology Co., Ltd. Fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Biotechnology Co., Ltd. BCA protein assay kit and Annexin V-FITC apoptosis detection kit were provided by Beyotime Institute of Biotechnology. Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Molecular Technologies, Inc. Antibodies to light chain 3 (LC3)-II rabbit mAb (cat. no. 3868s), p62 rabbit mAb (cat. no. 5114), phosphorylated (p-)AKT rabbit mAb (cat. no. 13038), p-mammalian target of rapamycin (mTOR) rabbit mAb (cat. no. 5536), β-actin rabbit mAb (cat. no. 8457) and anti-rabbit IgG alkaline phosphatase (AP)-linked antibodies (cat. no. 7054) were obtained from Cell Signaling Technology, Inc.

Du J., Li J., Song D., Li Q., Li L., Li B, & Li L. (2020). Matrine exerts anti-breast cancer activity by mediating apoptosis and protective autophagy via the AKT/mTOR pathway in MCF-7 cells. Molecular Medicine Reports, 22(5), 3659-3666.

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Determination of Phytochemical Compounds

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Acetic acid (Tianjin Chemical Reagent Company, China); xylene (Shanghai Chemical Reagent Company, China); aspirin (Shenyang Aojina Pharmaceutical Company, China); diphenoxylate (Xinxiang Changle Pharmaceutical Company, China); castor oil (Shanghai Aladdin Industrial Corporation, China); and acetonitrile and methanol (HPLC grade) were purchased from Fisher Scientific (England); quercetin, berberine, atractylodin, matrine and patehouli aleohal were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Purity of all ≥98%, China).

Shang X., Miao X., Yang F., Li B., Guo X., Pan H., Zhang Y, & Zhang J. (2018). The Anti-diarrheal Activity of the Non-toxic Dihuang Powder in Mice. Frontiers in Pharmacology, 9, 1037.

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Characterization of Botanical Compounds

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Chlorogenic acid, neoChlorogenic acid, cryptoChlorogenic acid, arctiin, arctigenin, tectoridin, tectorigenin, iridin, irisflorentin, irigenin, matrine, oxymatrine, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, and galuteolin were obtained from the Shanghai YuanYe Biotechnology Co., Ltd. (Shanghai, China) for use as references. Glycyrrhizic acid and glycyrrhetinic acid were purchased from China’s National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). The purity of the reference compounds was >98% based on high-performance liquid chromatography (HPLC) analysis. SJLYKL was prepared by the Shanghai Liantang pharmacy (Shanghai, China). Deionized water was purified using a Milli-Q system (Millipore, Bedford, MA, USA). Ammonium acetate (HPLC grade; purity ≥98.0%) and acetic acid (HPLC grade; purity ≥99.7%) were provided by ANPEL Laboratory Technologies (Shanghai, China). Chromatographic grade methanol and acetonitrile were purchased from Merck (Darmstadt, Germany).

Gu J., Wu W., Huang M., Long F., Liu X, & Zhu Y. (2018). Application of High-Performance Liquid Chromatography Coupled with Linear Ion Trap Quadrupole Orbitrap Mass Spectrometry for Qualitative and Quantitative Assessment of Shejin-Liyan Granule Supplements. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(4), 884.

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Inflammatory Response Modulation Protocol

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Incomplete Freund's adjuvant (IFA) and Bacillus Calmette-Guérin (BCG) were purchased from Rebio Scientiﬁc (Shanghai, China). TNF-α, IL-1β, IL-10, and IL-6 ELISA kits were the products of Multi Science (Hangzhou, China). Biochemical quantification kits for the determination of malondialdehyde (MDA), reduced glutathione (GSH), total superoxide dismutase activity (SOD), total antioxidant capacity (T-AOC), nitric oxide (NO), nitric oxide synthase (inducer) (iNOS), and carbonic oxide (CO) were brought from Jiancheng Bioengineering Institute (Jiangsu, China). The primary antibodies used in immunoblotting and immunohistochemical experiments including anti-MMP3, COX-2, TLR4, SIRT1, PPAR-γ, p65, p-p65, and β-actin antibodies together with secondary antibodies were provided by ABclonal Technology (Wuhan, China). Fluorescein-tagged anti-CD3, CD4, and IL-17α antibodies for the use of flow cytometry analysis were supplied by Beyotime Biotech (Nantong, Jiangsu, China). Rat peripheral blood mononuclear cell (PBMC) isolation kit, TaqMan RT-qPCR kit, and cDNA synthesis kit were purchased from Solarbio Technologies (Beijing, China). All the solvents were of analytical grade and supplied by Merck Chemicals (Shanghai, China). High-purity compounds (>98%) kaempferol, paeoniflorin, matrine, sophocarpine, and sinomenine were all obtained from Yuanye Bio-Technology (Shanghai, China).

Li D.F., Xie C.Q., Wu Y.J., Shi C., Ji C.F., Hu Y.F., Liao Q, & Li Y. (2022). Xiaoyao-Qingluoyin Cure Adjuvant-Induced Arthritis by Easing LPS Response-Related Pathway-Mediated Immune Abnormality. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 8536998.

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Matrine Regulates Akt and GSK-3β Signaling

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Matrine (>95% purity; Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China) was dissolved in PBS (10 mg/ml). Antibodies against phosphorylated (p)-Akt (Thr308) (sc-16646-R; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), Akt (2620S; Cell Signaling Technology, Inc., Danvers, MA, USA), β-actin (66009–1-Ig; Proteintech Group, Inc., Chicago, IL, USA), GSK-3β (9832; Cell Signaling Technology, Inc.), p-GSK-3β (9323; Cell Signaling Technology, Inc.) were used in the present study. The secondary anti-rabbit-horseradish peroxidase (HRP) (ab6721) or anti-mouse-HRP antibodies (ab6728) were purchased from Abcam (Cambridge, UK).

Xie W., Lu J., Lu Q., Wang X., Long H., Huang J, & Guo Z. (2018). Matrine inhibits the proliferation and migration of lung cancer cells through regulation of the protein kinase B/glycogen synthase kinase-3β signaling pathways. Experimental and Therapeutic Medicine, 16(2), 723-729.

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