Rat il 1β elisa kit

After centrifugation (1,000 × g; 25°C; 5 min), the cell supernatant of each subgroup was collected to analyze the levels of MDA, GSH-Px, SOD and the inflammatory factors IL-6, IL-1β and TNF-α. The following kits were used according to the manufacturer's instructions: Micro MDA assay kit (cat. no. BC0020; Beijing Solarbio Science & Technology Co., Ltd.), GSH-Px assay kit (cat. no. BC1195; Beijing Solarbio Science & Technology Co., Ltd.), SOD activity detection kit (cat. no. BC0170; Beijing Solarbio Science & Technology Co., Ltd.), rat IL-1β ELISA kit (cat. no. RLB00; R&D Systems, Inc.), rat IL-6 Quantikine ELISA kit (cat. no. R6000B; R&D Systems, Inc.) and rat TNFα Quantikine ELISA kit (cat. no. RTA00; R&D Systems, Inc.).

Before the rats were killed under excessive anesthesia, blood was collected through the abdominal aorta, and then centrifuged at 4°C, retained the supernatant and store at −20°C for later use. TNF‐α and IL‐1β levels and in rat was determined using rat TNF‐α ELISA kit and rat IL‐1β ELISA kit according to manufacturer's instructions (Solarbio).

The levels of BUN and creatinine in rat plasma were determined using a Urea assay kit (cat. no. C013-2-1) and Creatinine assay kit (cat. no. C011-1-1), respectively. Kidney tissues were rinsed in 100 mg/ml normal saline, then weighed and homogenized. MDA content and activities of CAT and MPO in the homogenate supernatant were determined. MDA in the supernatant of homogenized renal tissues from rats was measured using a MDA assay kit (cat. no. A003-1-2). The CAT enzymatic activity was measured using the CAT assay kit (cat. no. A007-1-1). The activity of MPO was measured by MPO assay kit (cat. no. A044-1-1). All kits were purchased from Nanjing Bioengineering Institute Co., Ltd and used according to the manufacturer's protocols. The levels of TNF-α and IL-1β in the rat plasma were measured using a Rat TNF-α ELISA kit (cat. no. SEKR-0009; Beijing Solarbio Science & Technology Co., Ltd.) and a Rat IL-1β ELISA kit (cat. no. SEKR-0002; Beijing Solarbio Science & Technology Co., Ltd.).