Anti ha tag sc805

Rabbit polyclonal anti-Angiomotin was previously described (Yi et al., 2011 (link)) (IB: 1:1500). The following antibodies are available commercially: anti-phospho-Angiomotin (ABS1045 from EMD Millipore; 1:2000). Anti-HA tag (sc805, 1:1000); monoclonal anti-Merlin (E-2, 1:500); polyclonal anti-Merlin (C-18, 1:500); polyclonal anti-YAP (H-125, 1:1000); polyclonal anti-Lamin A/C (sc-6215 (N-18), 1:500); polyclonal anti-EGFR (sc03, 1:1000) were from Santa Cruz Biotechnologies. Anti-Flag tag (F1804, 1:1000); anti-GAPDH (68795, 1:10000); anti-tubulin (T5168, 1:1000); anti-actin (A4700; 1:10000) were from Sigma. Anti-V5 tag (ab27671, 1:2000) from Abcam. Anti-6x His tag (MA1-21315, 1:2000) from Thermo Scientific. Anti-phospho-YAP (4911, 1:1000); anti-pan-Tead (13295; 1:1000) from Cell Signaling Technologies. Anti-Na⁺/K⁺ATPase (a-5, 1:2500) from Developmental Studies Hybridoma Bank (University of Iowa). For immunofluorescence the following antibodies were used at the stated concentrations: rabbit monoclonal anti-YAP D8H1X (14074, 1:50), Cell Signaling Technologies; mouse monoclonal anti-Merlin (E-2; 1:50), Santa Cruz Biotechnologies; Rabbit polyclonal anti-Angiomotin (1: 200).

Anti-HDAC1 antibody was previously generated in our laboratory^{69 (link)}. Anti-FLAG M2 (F1804) and anti-β-actin (A1978) antibodies were purchased from Sigma-Aldrich (St. Louis, MO). Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, HRP-60004) antibody was from Proteintech (Rosemont, IL). Anti-Lamin A (sc-20680) and anti-HA tag (sc-805) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX). Anti-EGFR (A300-388A) antibody was from Bethyl Laboratories (Montgomery, TX). Anti-phospho-EGFR (Y1173) (AF1095) antibody was from R&D Systems (Minneapolis, MN). Anti-Bim (2876) and anti-Myc tag (9B11, 2276) antibodies were from Cell Signaling Technology (Danvers, MA). Phosphotyrosine antibody cocktail (13-6620) was from Invitrogen (Thermo Fisher Scientific, Waltham, MA). Mouse (NA931) and Rabbit (NA934) IgG HRP-linked antibodies were purchased from GE Healthcare (Chicago, IL).
Rhodamine Phalloidin (PHDR1) was from Cytoskeleton (Denver, CO). Epidermal Growth Factor (EGF; cat. 236-EG) was purchased from R&D Systems. Gefitinib (ZD1839) was from Cayman Chemical Company (Ann Arbor, MI). AG-1478 (A8357) and MG-132 (A2585) were from ApexBio (Houston, TX). Cycloheximide (C1988) and leptomycin B (L2913) were purchased from Sigma-Aldrich.