Fl auto cell imaging system

Manufactured by Leica

The FL Auto Cell Imaging System is a laboratory equipment designed for automated cell imaging. It provides high-quality fluorescence microscopy images of cell samples.

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Lab products found in correlation

Dm il led, by Leica (1 mentions) Dmlb light microscope, by Leica (1 mentions) Ix81 inverted microscope, by Olympus (1 mentions) Fluoview fv500 laser scanning confocal system, by Olympus (1 mentions)

2 protocols using fl auto cell imaging system

Quantifying Osteoclast Bone Resorption

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Dentine Discs (Immunodiagnostics Systems Ltd., UK) were used to measure the functional capacity of differentiated OCs. Briefly, non-adherent BM was cultured on dentine discs for 14 days with medium refreshed every fourth day. Vehicle controls and treatments were commenced on day 4 and added with every medium replacement. OCs were lysed on dentine discs by incubation with distilled H₂O prior to visualization of resorption pits using black ink. The area of resorption was measured as a percentage of the total area using ImageJ software. Images were obtained on an EVOS FL Auto Cell Imaging System or a Leica DM IL LED at 10× magnification with scale bars set at 400 µm.

Doonan J., Lumb F.E., Pineda M.A., Tarafdar A., Crowe J., Khan A.M., Suckling C.J., Harnett M.M, & Harnett W. (2018). Protection Against Arthritis by the Parasitic Worm Product ES-62, and Its Drug-Like Small Molecule Analogues, Is Associated With Inhibition of Osteoclastogenesis. Frontiers in Immunology, 9, 1016.

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Live-cell Imaging Techniques Used

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Live-cell imaging was performed using an EVOS FL Auto Cell Imaging System or the Leica DMLB light microscope with differential interference contrast optics and a Leica DC300 video camera; a Nikon A1 Plus laser scanning confocal mounted on a Nikon 90i compound microscope (software version: NIS Elements 4.13 Build 914); or an Olympus Fluoview FV500 Laser Scanning Confocal System mounted on an Olympus Inverted IX81 microscope equipped with external photomultiplier tube (PMT), as described in detail, with strain and set-up information, in Supporting Information Methods S7.

Li G., Qi X., Sun G., Rocha R.O., Segal L.M., Downey K.S., Wright J.D, & Wilson R.A. (2020). Terminating rice innate immunity induction requires a network of antagonistic and redox-responsive E3 ubiquitin ligases targeting a fungal sirtuin. The New phytologist, 226(2).

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