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Annexin 5 fluorescein isothiocyanate fitc apoptosis kit

Manufactured by Dojindo Laboratories
Sourced in Japan

The Annexin V-Fluorescein Isothiocyanate (FITC) Apoptosis Kit is a laboratory tool designed to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a marker of apoptotic cells, and is conjugated with the fluorescent dye FITC for visualization and analysis purposes.

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2 protocols using annexin 5 fluorescein isothiocyanate fitc apoptosis kit

1

Apoptosis Induction Assay

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Approximately 2×106 cells were plated in 10-cm dishes. After the cells attached, they were exposed to DMSO, crizotinib, chidamide or the combination of the two drugs for 48 hr. Then, the cell suspension was collected and incubated with Annexin V-FITC and PI in the dark for 15 min according to the protocol of the Annexin V-Fluorescein Isothiocyanate (FITC) Apoptosis Kit (Dojindo, Kumamoto, Japan). These samples were analyzed by flow cytometry (BD Biosciences, NJ, USA) within 1 hr.
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2

Apoptosis Quantification by Flow Cytometry

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Approximately 2×10 6 cells were plated in 10-cm dishes. After the cells attached, they were exposed to DMSO, crizotinib, chidamide or the combination of the two drugs for 48 hr. Then, the cell suspension was collected and incubated with Annexin V-FITC and PI in the dark for 15 min according to the protocol of the Annexin V-Fluorescein Isothiocyanate (FITC) Apoptosis Kit (Dojindo, Kumamoto, Japan). These samples were analyzed by flow cytometry (BD Biosciences, NJ, USA) within 1 hr.
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