Masson goldner trichrome

Manufactured by Merck Group

Sourced in Germany

The Masson-Goldner trichrome is a histological staining technique used for the identification and visualization of various tissue components, including collagen, muscle, and other connective tissues. It provides a clear differentiation between these structures, allowing for detailed analysis and assessment of tissue samples.

Automatically generated - may contain errors

Lab products found in correlation

Coolsnap pro camera, by Teledyne (2 mentions) Dmrbe microscope, by Media Cybernetics (2 mentions) Galectin 3, by Media Cybernetics (2 mentions) Anti galectin 3, by BD (2 mentions) Oil red o, by Merck Group (2 mentions) Image pro plus, by Media Cybernetics (2 mentions) Optical microscope, by Olympus (1 mentions) Mirax scan, by Zeiss (1 mentions) Pannoramic viewer software, by 3DHISTECH (1 mentions) Grinding system, by EXAKT (1 mentions) Technovit 9100 new, by Kulzer (1 mentions)

5 protocols using masson goldner trichrome

Histomorphometric Analysis of Bone Regeneration

Check if the same lab product or an alternative is used in the 5 most similar protocols

Histomorphometric analysis was performed to evaluate the percentage of new bone formation within the bone defect area. Sections were stained with Masson-Goldner Trichrome (Merck KGaA, Darmstadt, Germany). All sections were evaluated under an optical microscope (Olympus, Milan, Italy) by two investigators unaware of the group assignment. The following criteria were applied to standardize the histomorphometric analysis: (1) the total defect area was identified by the anterior and posterior margin of the surgical defect area and was delimited; (2) the area of newly formed bone was delineated within the selected total area.
Percentage of new bone (% NB) formation was calculated as area of newly formed bone expressed as percentage of the total defect area. Digitally fixed images were randomly analyzed using an image analyzer (Image Pro Premier 9.1; Immagini e Computer, Milan, Italy). The measurements were made as percentage of area in five sections for each sample.

Buffoli B., Favero G., Borsani E., Boninsegna R., Sancassani G., Labanca M., Rezzani R., Nocini P.F., Albanese M, & Rodella L.F. (2017). Sodium-DNA for Bone Tissue Regeneration: An Experimental Study in Rat Calvaria. BioMed Research International, 2017, 7320953.

+ Open protocol

+ Expand

Quantitative Analysis of Atherosclerosis in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were fed a normal mouse chow (B & K Universal Ltd, UK) and harvested at 9 or 16 weeks of age. For the high fat diet cohort, 8 week old mice were fed a western-type diet for 8 weeks and harvested at 16 weeks of age. Atherosclerotic lesion size was assessed in paraffin-embedded aortic root sections stained with Masson-Goldner trichrome (Merck, Germany). The average lesion size was calculated from three sections taken at 100 μm intervals starting from the section showing all three aortic cusps. The infiltration of macrophages into aortic lesions was analysed using anti-Galectin-3 (BD Pharmingen, UK) immunostaining (Supplementary Table 4). Aortic lipid deposition was assessed in fixed aortas stained with Oil red O (Sigma-Aldrich, UK) from mice fed a chow diet for 16 weeks. Aortic roots were visualised and imaged (coolSNAP-pro camera, Roper Scientific, Leica DMRBE microscope and the lesion area and Galectin-3 positive areas were quantified from digitized microscopic images using Image-Pro Plus (Media Cybernetics, USA).

Patel J., McNeill E., Douglas G., Hale A.B., de Bono J., Lee R., Iqbal A.J., Regan-Komito D., Stylianou E., Greaves D.R, & Channon K.M. (2015). RGS1 regulates myeloid cell accumulation in atherosclerosis and aortic aneurysm rupture through altered chemokine signalling. Nature Communications, 6, 6614.

+ Open protocol

+ Expand

Quantitative Analysis of Atherosclerosis in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were fed a normal mouse chow (B & K Universal, UK) and harvested at 9 or 16 weeks of age. For the high-fat diet cohort, 8-week-old mice were fed a western-type diet for 8 weeks and harvested at 16 weeks of age. Atherosclerotic lesion size was assessed in paraffin-embedded aortic root sections stained with Masson-Goldner trichrome (Merck, Germany). The average lesion size was calculated from three sections taken at 100-μm intervals starting from the section showing all three aortic cusps. The infiltration of macrophages into aortic lesions was analysed using anti-Galectin-3 (BD Pharmingen, UK) immunostaining (Supplementary Table 4). Aortic lipid deposition was assessed in fixed aortas stained with Oil red O (Sigma-Aldrich) from mice fed a chow diet for 16 weeks. Aortic roots were visualized and imaged (coolSNAP-pro camera, Roper Scientific, Leica DMRBE microscope and the lesion area and Galectin-3-positive areas were quantified from digitized microscopic images using Image-Pro Plus (Media Cybernetics, USA).

+ Open protocol

+ Expand

Histological Analysis of Wound Healing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Each formalin fixed scar was divided in two halves by cutting it perpendicular to the incision line in the middle of the scar.
To minimize the influence of the sutures to the wound healing process, the staining was performed on 4 μm formalin fixed paraffin embedded sections, which were extracted from the middle of the wound. The tissue was stained with hematoxylin and eosin (H.E.) as well as with Masson‐Goldner trichrome (Merck, Darmstadt, Germany) 16. The stained samples were then scanned using the MIRAX SCAN (Carl Zeiss Microimaging GmbH, Jena, Germany). Microscopic measurement and histological examination were carried out using Pannoramic Viewer software (3DHISTECH Kft., Budapest, Hungary). In all Masson‐Goldner trichrome stained sections, the width of the subepithelial fibrosis and the depths of the wound were measured. In addition, a line was charted all around the fibrosis‐zone and the area was calculated.
The measurements were performed by three different examiners, who were experienced in histological evaluations. The observers were blinded, whereas all of them were involved in the study and could have possibly recognized some stained samples.

Petersen H., Tavakoli F., Kruber S., Münscher A., Gliese A., Hansen N., Uschold S., Eggert D., Robertson W.D., Gosau T., Sehner S., Kwiatkowski M., Schlüter H., Schumacher U., Knecht R, & Miller R.J. (2016). Comparative study of wound healing in rat skin following incision with a novel picosecond infrared laser (PIRL) and different surgical modalities. Lasers in Surgery and Medicine, 48(4), 385-391.

+ Open protocol

+ Expand

Histological Examination of Bone-Implant Interface

Check if the same lab product or an alternative is used in the 5 most similar protocols

For histological examination, each bone-implant specimen was placed in 4% phosphate-buffered saline (PBS)buffered formalin, dehydrated in a graded series of alcohol and embedded in methylmethacrylate (Technovit 9100 New ® , Kulzer, Germany), as described previously. [8] [9] [10] [11] For staining the bone-implant, specimens were cut using a diamond saw and ground to a thickness of approx. 100 µm with a grinding system (Exakt Apparatebau, Norderstedt, Germany) as described by Donath and Breuner. 12 The specimens were then stained with Masson-Goldner trichrome (Merck KGaA, Darmstadt, Germany). for differentiation between collagen and bone tissue, and a histomorphometric analysis was performed by light microscopy. A blind test was conducted at the same time, using identical staff, equipment and chemicals.

Kubasiewicz-Ross P., Hadzik J, & Dominiak M. (2018). Osseointegration of zirconia implants with 3 varying surface textures and a titanium implant: A histological and micro-CT study. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 27(9).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!