Immunohistochemistry was performed on paraffin-embedded tissues. Target retrieval was carried out by heating the tissue in a citrate buffer (DakoCytomation, Glostrup, Denmark) at pH 6. Endogenous peroxidase was inactivated by incubating the specimen for 10 minutes at room temperature in 0.03% H2O2. The sections were incubated overnight at 4°C with phosphate-buffered saline containing 1:50 rabbit anti-ALAS1 (HPA035860, Sigma-Aldrich, St Louis, MO). The immunoreactive proteins were visualized using the Envision + HRP system (AEC; DakoCytomation). Finally, the tissue sections were counterstained with hematoxylin and mounted using aqueous mounting medium (Dako, Glostrup, Denmark). The primary antibodies were replaced by equal concentrations of rabbit or mouse IgG (Dako) as negative controls. A blocking peptide (ABIN973092; Antibodies-online GmbH, Aachen, Germany) has been used to monitor the specificity of the staining.
Rabbit or mouse igg
Manufactured by Agilent Technologies
Rabbit or mouse IgG is a laboratory reagent used for various immunological techniques. It serves as a source of immunoglobulin G (IgG) antibodies derived from rabbits or mice. IgG antibodies are a class of antibodies that play a crucial role in the adaptive immune response. The core function of rabbit or mouse IgG is to provide a standardized and consistent source of IgG for research purposes, such as in immunoassays, affinity purification, and other applications where IgG antibodies are required.
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Lab products found in correlation
2 protocols using rabbit or mouse igg
1
Immunohistochemical Analysis of ALAS1
2
Immunostaining of Kidney Nephron Segments
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Tissue sections (4 µm) have been performed and stained by the Yasue procedure to reveal tissue calcifications. To assess the topography of incipient plugs, Yasue staining has been followed by classical immunostaining. Proximal convoluted tubules and descending loops of Henle were immunostained with a rabbit anti-aquaporin 1 (AQP1) antibody (1:600, SC-20810 clone H-55, Santa Cruz Biotechnology). Ascending loops of Henle were immunostained with a mouse monoclonal anti-Barttin antibody (1:100, SC-365161 clone A-3, Santa Cruz Biotechnology). Distal convoluted tubules and, to a lesser extent, collecting ducts were immunostained with rabbit anti-WNK 4 antibody (1:400, clone NB600-284, Novus Bio). Collecting ducts were immunostained with a goat anti-aquaporin 2 (AQP2) antibody (1:200, SC-9882 clone C-17, Santa Cruz Biotechnology). Samples were revealed with Single Stain MAX PO (mouse, rabbit or goat) Histofine (Nichirei Biosciences, Japan). For negative controls, the primary antibodies were replaced by an equal concentration of rabbit or mouse IgG (Dako).
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