Cd11b dtr b6 fvb tg itgam dtr egfp 34lan j

Manufactured by Jackson ImmunoResearch

Sourced in Germany

CD11b-DTR (B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J) is a genetically engineered mouse strain that expresses the diphtheria toxin receptor (DTR) under the control of the CD11b promoter, which drives expression in myeloid cells. This strain allows for the selective depletion of CD11b-positive cells, such as monocytes, macrophages, and dendritic cells, upon administration of diphtheria toxin.

Automatically generated - may contain errors

Lab products found in correlation

C57bl 6j, by Jackson ImmunoResearch (1 mentions) Nod cg prkdcscid il2rgtm1wjl szj, by Jackson ImmunoResearch (1 mentions) Balb cj, by Jackson ImmunoResearch (1 mentions) Diphtheria toxin, by Merck Group (1 mentions) B6.129s h2dlab1 ea, by Jackson ImmunoResearch (1 mentions) Cd11c dtr b6 fvb tg itgax dtr egfp 57lan j, by Jackson ImmunoResearch (1 mentions) C57bl 6 wt, by Charles River Laboratories (1 mentions)

Spelling variants of this product

CD11b-DTR (7 citations) B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J, (3 citations) CD11b-/- (3 citations) CD11b-DTR (FVB-Tg(ITGAM-DTR/EGFP)34Lan/J (2 citations) FVB-Tg(ITGAM-DTR/ EGFP)34Lan/J (2 citations)

4 protocols using cd11b dtr b6 fvb tg itgam dtr egfp 34lan j

Murine Immune Cell Isolation Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

BALB/cJ (000651), C57BL/6J (000664), CD11b-DTR (B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J; 006000), and NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ (005557, all 6–12 wk old) mice were purchased from the Jackson Laboratories and used as immune cell donors or graft recipients for different assays. Sirpa⁻^/⁻ C57BL/6 mice were generated by Y. Liu as previously described (Bian et al., 2016 (link)). Animals were randomly assigned to experimental groups. The number of animals per experimental group is presented in each figure. Mice received humane care in compliance with the University of California, San Francisco Institutional Animal Care and Use Committee and performed according to local guidelines.

Deuse T., Hu X., Agbor-Enoh S., Jang M.K., Alawi M., Saygi C., Gravina A., Tediashvili G., Nguyen V.Q., Liu Y., Valantine H., Lanier L.L, & Schrepfer S. (2021). The SIRPα–CD47 immune checkpoint in NK cells. The Journal of Experimental Medicine, 218(3), e20200839.

+ Open protocol

+ Expand

Depletion of CD11b+ cells in mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eight- to 10-week-old wild-type (WT) C57BL/6 (B6, H2^b) mice were purchased from Charles River Laboratories (Wilmington, Mass). MC^−/− (WBB6F1/J-Kit^W/Kit^W-v/J [Kit^W/Kit^W-v] and Kit^W-sh/HNihrJaeBsmJ [kit^Wsh/Kit^Wsh]), MHC class II^−/− (B6.129S-H2dlAb1-Ea), Wiskott-Aldrich syndrome protein (WASP)^−/− (B6.129S6-Was^tm1Sbs/J), and CD11b-DTR (B6.FVB-Tg[ITGAM-DTR/EGFP]34Lan/J) mice were purchased from Jackson Laboratory (Bar Harbor, Me). Recombination-activating gene 2 (Rag2)^−/− γc^−/− (B10; B6-.Rag2^tmlFwaII2rg^tm1Wjl), Rag2^−/− , and 5C.C7 Rag2^−/− mice (both on B10.A background) were purchased from Taconic Biosciences (Albany, NY). For CD11b⁺ cell depletion with diphtheria toxin treatment, CD11b-DTR transgenic mice weighing 25 to 30 g were injected with diphtheria toxin (25 ng/g body weight; Sigma-Aldrich, St Louis, Mo) 24 hours before and 72 hours after beginning NAD⁺ or PBS administration.

Biefer H.R., Heinbokel T., Uehara H., Camacho V., Minami K., Nian Y., Koduru S., Fatimy R.E., Ghiran L., Trachtenberg A.J., de la Fuente M.A., Azuma H., Akbari O., Tullius S.G., Vasudevan A, & Elkhal A. (2018). Mast cells regulate CD4+ T-cell differentiation in the absence of antigen presentation. The Journal of allergy and clinical immunology, 142(6), 1894-1908.e7.

+ Open protocol

+ Expand

Generating Chimeric Mice via Irradiation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

IFNAR^-/- mice (C57Bl/6 background) were obtained from the Friedrich Loeffler Institute, Isle of Riems, Germany, and bred in the Specific Pathogen Free animal facility of the Bernhard Nocht Institute for Tropical Medicine. C57BL/6J and CD45.1⁺ congenic B6 mice, CD11b-DTR (B6.FVB-Tg(ITGAM-DTR/EGFP)₃₄Lan/J), and CD11c-DTR (B6.FVB-Tg(ITGAX-DTR/EGFP)₅₇Lan/J) mice were purchased from Jackson Laboratories and bred at the Heinrich Pette Institute animal facility. Bone marrow chimeric mice were generated at the Heinrich Pette Institute animal facility. Four to eight week old female mice were irradiated twice (550 rad, 4 hr apart by a Caesium source) and reconstituted with 2x10⁶ bone marrow cells from donor mice as previously described [20 (link)]. Split irradiation reduced the number of animals succumbing to irradiation to under 3%. Three to five mice per group were kept together in individually ventilated cages (IVC) and had ad libitum access to food and water. Engraftment in peripheral blood was evaluated 4 weeks after reconstitution by flow cytometry and the experiments were performed 4–5 weeks after transplantation. Chimeric mice showing reconstitution of donor hematopoietic cells above 85% were selected for the experiments. More than 90% of chimeric mice generated had this level of donor engraftment or higher.

Oestereich L., Lüdtke A., Ruibal P., Pallasch E., Kerber R., Rieger T., Wurr S., Bockholt S., Pérez-Girón J.V., Krasemann S., Günther S, & Muñoz-Fontela C. (2016). Chimeric Mice with Competent Hematopoietic Immunity Reproduce Key Features of Severe Lassa Fever. PLoS Pathogens, 12(5), e1005656.

+ Open protocol

+ Expand

Mouse Transgenic Model for Immune Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

All procedures were approved by the State Office for Nature, Environment and Consumer Protection of North Rhine-Westphalia (reference number 81-02.04. 2020.A227) and conducted in accordance with German Animal Welfare Law. All experiments were performed using male (20 to 30 g) wild-type (WT) C57BL/6 (Charles River Laboratories, Sulzfeld, Germany) and transgenic CD11b-DTR (B6.FVB-Tg (ITGAM-DTR/EGFP)34 Lan/J, Jackson Laboratory, stock #006000, Bar Harbor, ME, USA, [23 (link)]) mice. Mice were maintained at the local animal facility for at least one week for acclimatization before undergoing treatment. Animals had ad libitum access to food and water and were kept under pathogen-free conditions and at a constant temperature (22 ± 2 °C) and air humidity (45–65%), with a 12 h light/dark cycle.

Winter M., Heitplatz B., Koppers N., Mohr A., Bungert A.D., Juratli M.A., Strücker B., Varga G., Pascher A, & Becker F. (2023). The Impact of Phase-Specific Macrophage Depletion on Intestinal Anastomotic Healing. Cells, 12(7), 1039.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!